LOCUS       sequence-330378-        9440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes a Cas9 protein as well as a sgRNA that targets 
            Msn2, which will create a double strand break and allow C-terminus 
            tagging via homologous recombination. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9440)
  TITLE     Heinemann Lab Plasmids
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HCP1
FEATURES             Location/Qualifiers
     source          1..9440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             873..4976
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             873..4976
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4989..5009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5013..5033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        5593..5668
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gene            5905..7260
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             6249..7058
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      7063..7260
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    7265..7768
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(7812..8627)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7812..8627)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8619..8638
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8852..9440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9341..9360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctggcctt
       61 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgccc
      121 ctgaattcgc atctagactg aactggccga taattgcaga cgaacggtga gtaaggaaag
      181 agtgaggaac tatcgcatac ctgcatttaa agatgccgat ttgggcgcga atcctttatt
      241 ttggcttcac cctcatacta ttatcagggc cagaaaaagg aagtgtttcc ctccttcttg
      301 aattgatgtt accctcataa agcacgtggc ctcttatcga gaaagaaatt accgtcgctc
      361 gtgatttgtt tgcaaaaaga acaaaactga aaaaacccag acacgctcga cttcctgtca
      421 tcctattgat tgcagcttcc aatttcgtca cacaacaagg tcctagcgac ggctcacagg
      481 ttttgtaaca agcaatcgaa ggttctggaa tggcgggaaa gggtttagta ccacatgcta
      541 tgatgcccac tgtgatctcc agagcaaagt tcgttcgatc gtactgttac tctctctctt
      601 tcaaacagaa ttgtccgaat cgtgtgacaa caacagcctg ttctcacaca ctcttttctt
      661 ctaaccaagg gggtggttta gtttagtaga acctcgtgaa acttacattt acatatatat
      721 aaacttgcat aaattggtca atgcaagaaa tacatatttg gtcttttcta attcgtagtt
      781 tttcaagttc ttagatgctt tctttttctc ttttttacag atcatcaagg aagtaattat
      841 ctacttttta caacaaatat aaaacaagat ctatggacaa gaagtattct atcggactgg
      901 atatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag gtgccctcta
      961 agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat ctgatcggag
     1021 ctctcctctt tgattcaggg gaaaccgctg aagcaacccg cctcaagcgg actgctagac
     1081 ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc tccaacgaaa
     1141 tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc gtggaagagg
     1201 ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg
     1261 agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc gacaaggcag
     1321 acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc cacttcctga
     1381 tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc cagctggtgc
     1441 agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc gatgccaagg
     1501 ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc gctcagctgc
     1561 cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt gggctgactc
     1621 ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg tccaaggaca
     1681 cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac gccgaccttt
     1741 tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca
     1801 ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat gagcaccacc
     1861 aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag tacaaggaaa
     1921 tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt gcctcccaag
     1981 aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc gaagagctcc
     2041 tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac aatgggagca
     2101 ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa gaggacttct
     2161 acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc cgcatcccct
     2221 actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc agaaagtcag
     2281 aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc agcgctcagt
     2341 cattcatcga acggatgact aacttcgata agaacctccc caatgagaag gtcctgccga
     2401 aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag gtgaaatatg
     2461 tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg
     2521 accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag gactatttca
     2581 agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa
     2641 gcctgggtac ttaccatgat ctgcttaaga tcatcaagga caaggatttt ctggacaatg
     2701 aggagaacga ggacatcctt gaggacattg tcctgactct cactctgttc gaggaccggg
     2761 aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa gtgatgaagc
     2821 aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc atcaacggaa
     2881 tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac ggattcgcta
     2941 atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag gacatccaga
     3001 aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat ctggctggtt
     3061 cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag ctggtcaagg
     3121 tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa aaccagacta
     3181 cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa gggattaagg
     3241 agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt cagaatgaga
     3301 agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa gagcttgata
     3361 tcaacaggct ctcagactac gacgtggacc atatcgtccc tcagagcttc ctcaaagacg
     3421 actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag tcagataacg
     3481 tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt ctgaacgcaa
     3541 agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc ggactgagcg
     3601 aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag attactaaac
     3661 atgtcgccca aatccttgac tcacgcatga ataccaagta cgacgaaaac gacaaactta
     3721 tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc agaaaggact
     3781 ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac gcatatctga
     3841 atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt
     3901 acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag caggagatcg
     3961 gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc aagaccgaaa
     4021 tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat ggggagactg
     4081 gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc
     4141 ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa
     4201 tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg gaccctaaga
     4261 agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg
     4321 aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc accattatgg
     4381 agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt tacaaggaag
     4441 tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg gagaatgggc
     4501 ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg gctctcccct
     4561 ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag gggagccccg
     4621 aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg gacgagatca
     4681 ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac ctcgataaag
     4741 tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct gagaatatca
     4801 tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac ttcgacacca
     4861 ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc ctcatccacc
     4921 agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga ggggatgagg
     4981 gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtataaggat
     5041 cctaactcga gattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc
     5101 ccatccttta cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac
     5161 gtatatatag actattattt atcttttaat gattattaag atttttatta aaaaaaaatt
     5221 cgctcctctt ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgtgctg
     5281 ccaaaccaga tgtcaacaca gctacaacga tgtgcttcag tattacattt tttgccttca
     5341 acgccttgat tgttctattt ttgctaataa taaatctatt tcatcggact aaaagtccat
     5401 tagttgtaag cggatttagc tcagttggga gagcgccaga ctgaagaaaa acttcggtca
     5461 agtcatctgg aggtcctgtg ttcgatccac agaattcgca gatggccggc atggtcccag
     5521 cctcctcgct ggcgccggct gggcaacacc ttcgggtggc gaatgggact ttatcaagac
     5581 tcataaaaaa cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     5641 acttgaaaaa gtggcaccga gtcggtgctt tttttatttt ttgtcactat tgttatgtaa
     5701 aatgccacct ctgacagtat ggaacgcaaa cttctgtcta gtggatagct ggatgcacac
     5761 actggcttaa gatgacaacg actagagatc tatgtgagga tcctaactcg agatcgagct
     5821 gagcagttac agagatgtta cgaaccacta gtgcactgca gtacagttta gcttgcctcg
     5881 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     5941 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     6001 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     6061 ggctcctcgc tccagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     6121 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     6181 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     6241 aaacaaaaat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg
     6301 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     6361 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     6421 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     6481 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     6541 cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     6601 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     6661 cttttaacag cgatcgcgta tttcgtctgg ctcaggcgca atcacgaatg aataacggtt
     6721 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     6781 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     6841 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     6901 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     6961 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     7021 aattgcagtt tcatttgatg ctcgatgagt ttttctaaag taactgacaa taaaaagatt
     7081 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa
     7141 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag
     7201 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg
     7261 gagtatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
     7321 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
     7381 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
     7441 gtattaatgc cgaattgttt catcttgtct gtgtagaaaa ccacacacga aaatcctgtg
     7501 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
     7561 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
     7621 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
     7681 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
     7741 ggcgcgtgta agttacaggc aagcgatccg tcccgagcgg ccgcgtgtta caaccaatta
     7801 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     7861 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     7921 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     7981 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     8041 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     8101 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     8161 attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa
     8221 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     8281 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     8341 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     8401 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     8461 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     8521 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     8581 tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta
     8641 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     8701 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     8761 gagttgaagg atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9421 gggggcggag cctatggaaa
//
