LOCUS       sequence-330382-        9192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes a Cas9 protein as well as a sgRNA that targets 
            the C-terminus of Whi5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9192)
  TITLE     Heinemann Lab Plasmids
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HCP8
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             862..4965
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             862..4965
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4978..4998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5002..5022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        5582..5657
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      6815..7012
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    7017..7520
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(7564..8379)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7564..8379)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8287..8306
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8371..8390
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8604..9192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9093..9112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcccc tgaattcgca
      121 tctagactga actggccgat aattgcagac gaacggtgag taaggaaaga gtgaggaact
      181 atcgcatacc tgcatttaaa gatgccgatt tgggcgcgaa tcctttattt tggcttcacc
      241 ctcatactat tatcagggcc agaaaaagga agtgtttccc tccttcttga attgatgtta
      301 ccctcataaa gcacgtggcc tcttatcgag aaagaaatta ccgtcgctcg tgatttgttt
      361 gcaaaaagaa caaaactgaa aaaacccaga cacgctcgac ttcctgtcat cctattgatt
      421 gcagcttcca atttcgtcac acaacaaggt cctagcgacg gctcacaggt tttgtaacaa
      481 gcaatcgaag gttctggaat ggcgggaaag ggtttagtac cacatgctat gatgcccact
      541 gtgatctcca gagcaaagtt cgttcgatcg tactgttact ctctctcttt caaacagaat
      601 tgtccgaatc gtgtgacaac aacagcctgt tctcacacac tcttttcttc taaccaaggg
      661 ggtggtttag tttagtagaa cctcgtgaaa cttacattta catatatata aacttgcata
      721 aattggtcaa tgcaagaaat acatatttgg tcttttctaa ttcgtagttt ttcaagttct
      781 tagatgcttt ctttttctct tttttacaga tcatcaagga agtaattatc tactttttac
      841 aacaaatata aaacaagatc tatggacaag aagtattcta tcggactgga tatcgggact
      901 aatagcgtcg ggtgggccgt gatcactgac gagtacaagg tgccctctaa gaagttcaag
      961 gtgctcggga acaccgaccg gcattccatc aagaaaaatc tgatcggagc tctcctcttt
     1021 gattcagggg aaaccgctga agcaacccgc ctcaagcgga ctgctagacg gcggtacacc
     1081 aggaggaaga accggatttg ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc
     1141 gacgacagct tcttccatag gctggaagaa tcattcctcg tggaagagga taagaagcat
     1201 gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg cctatcacga gaaataccca
     1261 accatctacc atcttcgcaa aaagctggtg gactcaaccg acaaggcaga cctccggctt
     1321 atctacctgg ccctggccca catgatcaag ttcagaggcc acttcctgat cgagggcgac
     1381 ctcaatcctg acaatagcga tgtggataaa ctgttcatcc agctggtgca gacttacaac
     1441 cagctctttg aagagaaccc catcaatgca agcggagtcg atgccaaggc cattctgtca
     1501 gcccggctgt caaagagccg cagacttgag aatcttatcg ctcagctgcc gggtgaaaag
     1561 aaaaatggac tgttcgggaa cctgattgct ctttcacttg ggctgactcc caatttcaag
     1621 tctaatttcg acctggcaga ggatgccaag ctgcaactgt ccaaggacac ctatgatgac
     1681 gatctcgaca acctcctggc ccagatcggt gaccaatacg ccgacctttt ccttgctgct
     1741 aagaatcttt ctgacgccat cctgctgtct gacattctcc gcgtgaacac tgaaatcacc
     1801 aaggcccctc tttcagcttc aatgattaag cggtatgatg agcaccacca ggacctgacc
     1861 ctgcttaagg cactcgtccg gcagcagctt ccggagaagt acaaggaaat cttctttgac
     1921 cagtcaaaga atggatacgc cggctacatc gacggaggtg cctcccaaga ggaattttat
     1981 aagtttatca aacctatcct tgagaagatg gacggcaccg aagagctcct cgtgaaactg
     2041 aatcgggagg atctgctgcg gaagcagcgc actttcgaca atgggagcat tccccaccag
     2101 atccatcttg gggagcttca cgccatcctt cggcgccaag aggacttcta cccctttctt
     2161 aaggacaaca gggagaagat tgagaaaatt ctcactttcc gcatccccta ctacgtggga
     2221 cccctcgcca gaggaaatag ccggtttgct tggatgacca gaaagtcaga agaaactatc
     2281 actccctgga acttcgaaga ggtggtggac aagggagcca gcgctcagtc attcatcgaa
     2341 cggatgacta acttcgataa gaacctcccc aatgagaagg tcctgccgaa acattccctg
     2401 ctctacgagt actttaccgt gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg
     2461 atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg caattgtgga ccttctgttc
     2521 aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa
     2581 tgcttcgact ctgtggagat tagcggggtc gaagatcggt tcaacgcaag cctgggtact
     2641 taccatgatc tgcttaagat catcaaggac aaggattttc tggacaatga ggagaacgag
     2701 gacatccttg aggacattgt cctgactctc actctgttcg aggaccggga aatgatcgag
     2761 gagaggctta agacctacgc ccatctgttc gacgataaag tgatgaagca acttaaacgg
     2821 agaagatata ccggatgggg acgccttagc cgcaaactca tcaacggaat ccgggacaaa
     2881 cagagcggaa agaccattct tgatttcctt aagagcgacg gattcgctaa tcgcaacttc
     2941 atgcaactta tccatgatga ttccctgacc tttaaggagg acatccagaa ggcccaagtg
     3001 tctggacaag gtgactcact gcacgagcat atcgcaaatc tggctggttc acccgctatt
     3061 aagaagggta ttctccagac cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc
     3121 cataaaccag agaacattgt catcgagatg gccagggaaa accagactac ccagaaggga
     3181 cagaagaaca gcagggagcg gatgaaaaga attgaggaag ggattaagga gctcgggtca
     3241 cagatcctta aagagcaccc ggtggaaaac acccagcttc agaatgagaa gctctatctg
     3301 tactaccttc aaaatggacg cgatatgtat gtggaccaag agcttgatat caacaggctc
     3361 tcagactacg acgtggacca tatcgtccct cagagcttcc tcaaagacga ctcaattgac
     3421 aataaggtgc tgactcgctc agacaagaac cggggaaagt cagataacgt gccctcagag
     3481 gaagtcgtga aaaagatgaa gaactattgg cgccagcttc tgaacgcaaa gctgatcact
     3541 cagcggaagt tcgacaatct cactaaggct gagaggggcg gactgagcga actggacaaa
     3601 gcaggattca ttaaacggca acttgtggag actcggcaga ttactaaaca tgtcgcccaa
     3661 atccttgact cacgcatgaa taccaagtac gacgaaaacg acaaacttat ccgcgaggtg
     3721 aaggtgatta ccctgaagtc caagctggtc agcgatttca gaaaggactt tcaattctac
     3781 aaagtgcggg agatcaataa ctatcatcat gctcatgacg catatctgaa tgccgtggtg
     3841 ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg agttcgtgta cggagactac
     3901 aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc aggagatcgg aaaggccacc
     3961 gcaaagtact tcttctacag caacatcatg aatttcttca agaccgaaat cacccttgca
     4021 aacggtgaga tccggaagag gccgctcatc gagactaatg gggagactgg cgaaatcgtg
     4081 tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac
     4141 atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta aggaatcaat cctccccaag
     4201 cgcaactccg acaagctcat tgcaaggaag aaggattggg accctaagaa gtacggcgga
     4261 ttcgattcac caactgtggc ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag
     4321 tctaagaagc tcaagagcgt gaaggaactg ctgggtatca ccattatgga gcgcagctcc
     4381 ttcgagaaga acccaattga ctttctcgaa gccaaaggtt acaaggaagt caagaaggac
     4441 cttatcatca agctcccaaa gtatagcctg ttcgaactgg agaatgggcg gaagcggatg
     4501 ctcgcctccg ctggcgaact tcagaagggt aatgagctgg ctctcccctc caagtacgtg
     4561 aatttcctct accttgcaag ccattacgag aagctgaagg ggagccccga ggacaacgag
     4621 caaaagcaac tgtttgtgga gcagcataag cattatctgg acgagatcat tgagcagatt
     4681 tccgagtttt ctaaacgcgt cattctcgct gatgccaacc tcgataaagt ccttagcgca
     4741 tacaataagc acagagacaa accaattcgg gagcaggctg agaatatcat ccacctgttc
     4801 accctcacca atcttggtgc ccctgccgca ttcaagtact tcgacaccac catcgaccgg
     4861 aaacgctata cctccaccaa agaagtgctg gacgccaccc tcatccacca gagcatcacc
     4921 ggactttacg aaactcggat tgacctctca cagctcggag gggatgaggg agctgatcca
     4981 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tataaggatc ctaactcgag
     5041 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
     5101 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
     5161 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
     5221 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgc caaaccagat
     5281 gtcaacacag ctacaacgat gtgcttcagt attacatttt ttgccttcaa cgccttgatt
     5341 gttctatttt tgctaataat aaatctattt catcggacta aaagtccatt agttgtaagc
     5401 ggatttagct cagttgggag agcgccagac tgaagaaaaa cttcggtcaa gtcatctgga
     5461 ggtcctgtgt tcgatccaca gaattcgcag atggccggca tggtcccagc ctcctcgctg
     5521 gcgccggctg ggcaacacct tcgggtggcg aatgggactt tctccacttc ggtatccgac
     5581 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     5641 tggcaccgag tcggtgcttt ttttattttt tgtcactatt gttatgtaaa atgccacctc
     5701 tgacagtatg gaacgcaaac ttctgtctag tggatagctg gatgcacaca ctggcttaag
     5761 atgacaacga ctagagatct atgtgaggat cctaactcga gatcgagctg agcagttaca
     5821 gagatgttac gaaccactag tgcactgcag tacagtttag cttgcctcgt ccccgccggg
     5881 tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag
     5941 ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc
     6001 atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct
     6061 ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc
     6121 gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact
     6181 tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata aacaaaaatg
     6241 ggtactacct tagatgatac agcctacaga tacagaacat cagtccctgg tgatgctgaa
     6301 gcaattgagg ctttagacgg ttcattcacc accgacaccg tctttagagt aaccgccacc
     6361 ggtgatggat ttaccttaag agaagtccca gtcgaccctc cattaactaa agtctttcca
     6421 gatgatgaat ctgatgacga aagcgacgac ggagaagatg gtgacccaga ttcaagaact
     6481 ttcgtagcat acggtgatga cggtgatttg gctggttttg tagtcgtttc ttattcaggt
     6541 tggaatagaa ggttgaccgt tgaagatata gaagtcgccc cagagcatag aggtcatggt
     6601 gtaggaagag ctttgatggg tttggctaca gaatttgcaa gagagagagg agccggtcat
     6661 ttatggttag aagttactaa tgttaacgcc cctgctatcc atgcttatag aagaatgggt
     6721 ttcacattat gtggtttaga tactgcttta tatgatggaa cagcatctga cggtgaacag
     6781 gccttgtata tgtctatgcc ttgcccttaa agtaactgac aataaaaaga ttcttgtttt
     6841 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     6901 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     6961 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tggagtatca
     7021 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag
     7081 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc
     7141 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat
     7201 gccgaattgt ttcatcttgt ctgtgtagaa aaccacacac gaaaatcctg tgattttaca
     7261 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
     7321 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
     7381 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
     7441 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
     7501 taagttacag gcaagcgatc cgtcccgagc ggccgcgtgt tacaaccaat taaccaattc
     7561 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     7621 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     7681 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     7741 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     7801 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     7861 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     7921 ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     7981 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     8041 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     8101 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     8161 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     8221 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     8281 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     8341 cggcctggag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     8401 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     8461 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
     8521 ggatcagtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9181 agcctatgga aa
//
