LOCUS       sequence-33042-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_c-MYC
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..150
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        131..149
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgga ctctagagga tcgaaccctt ttggaccctc
      121 gtacagaagc taatacgact cactataggg aaataagaga gaaaagaaga gtaagaagaa
      181 atataagagc caccatgccc ctcaacgtta gcttcaccaa caggaactat gacctcgact
      241 acgactcggt gcagccgtat ttctactgcg acgaggagga gaacttctac cagcagcagc
      301 agcagagcga gctgcagccc ccggcgccca gcgaggatat ctggaagaaa ttcgagctgc
      361 tgcccgcgcc gcccctgtcc cctagccgcc gctccgggct ctgctcgccc tcctacgttg
      421 cggtcacacc cttctccctt cggggagaca acgacggcgg tggcgggagc ttctccacgg
      481 ccgaccagct ggagatggtg accgagctgc tgggaggaga catggtgaac cagagtttca
      541 tctgcgaccc ggacgacgag accttcatca aaaacatcat catccaggac tgtatgtgga
      601 gcggcttctc ggccgccgcc aagctcgtct cagagaagct ggcctcctac caggctgcgc
      661 gcaaagacag cggcagcccg aaccccgccc gcggccacag cgtctgctcc acctccagct
      721 tgtacctgca ggatctgagc gccgccgcct cagagtgcat cgacccctcg gtggtcttcc
      781 cctaccctct caacgacagc agctcgccca agtcctgcgc ctcgcaagac tccagcgcct
      841 tctctccgtc ctcggattct ctgctctcct cgacggagtc ctccccgcag ggcagccccg
      901 agcccctggt gctccatgag gagacaccgc ccaccaccag cagcgactct gaggaggaac
      961 aag
//
