LOCUS       sequence-330423-       11514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of GFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->sGFP_PGK-->puroR
FEATURES             Location/Qualifiers
     source          1..11514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    578..695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        761..1939
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          992..1930
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1887..1907
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1956..1965
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2013..2015,2016..2726)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        2810..3309
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2845..2868)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3242..3261
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3319..3915
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3319..3338)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3815..3835
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4006..4026)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4424..4435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4604..4837
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4862..4880)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4863..4880)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5170..5189)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5403..5422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5522..5544
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5582..5600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5668..5772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5773..5841,5842..6633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5991..6010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6804..7392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7293..7312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7546..7563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7633..7653)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7638..7967
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7818..7953
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9101..9166
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9296..9316
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9741..9875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9778..9797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9832..9851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10043..10676
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10723..10848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11345..11514,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      601 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      661 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      721 atccagtttg gatcgataag cttgatatcg aattccgtga ggctccggtg cccgtcagtg
      781 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      841 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      901 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      961 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     1021 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     1081 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     1141 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     1201 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     1261 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1321 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1381 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1441 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1501 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1561 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1621 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1681 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1741 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1801 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1861 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1921 tccatttcag gtgtcgtgag aattctcacg cggccgccac catgggcgtt aaagtccttt
     1981 tcgccctgat ctgcattgcc gtcgcagagg ccgtttctaa gggtgaagaa ttgttcaccg
     2041 gagttgtgcc catcctggtt gagctggacg gagacgttaa cggacacaag ttctcagtga
     2101 gcggcgaagg agagggcgac gctacgtacg gtaaactgac gttgaagttc atctgtacaa
     2161 caggaaagct gccagtgccc tggcctacac tcgtgaccac cctcacctac ggagtccagt
     2221 gcttctcccg gtaccccgac cacatgaaac agcacgattt cttcaagtcc gcgatgcctg
     2281 agggctatgt tcaggaacgc accatattct tcaaggacga cggtaactat aaaaccaggg
     2341 ctgaagttaa gttcgagggg gacacgttgg taaatagaat tgagttgaaa ggaatcgatt
     2401 tcaaagaaga cggcaatatt ttgggccaca agctggagta caactacaac agccacaacg
     2461 tgtatattat ggctgataag cagaagaacg gcatcaaggt gaacttcaaa atccgacaca
     2521 atatcgagga tggctctgtg caactggccg accattacca gcaaaacacg cctatcggcg
     2581 acggtccggt tttgctgcca gataaccact acctctccac gcaatcagcc ctctccaagg
     2641 atcctaacga aaaaagagac catatggttc tgcttgaatt cgtgacggct gctgggatca
     2701 ccctgggaat ggatgagctg tataagtgat taattaaggc cgcaactccc acctgcaaca
     2761 tgcgtgactg actgaggccg cgactctaga gtcgacctgc aggtctaccg ggtaggggag
     2821 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2881 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2941 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     3001 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg actcactagt
     3061 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     3121 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     3181 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3241 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3301 ggcctttcgt ccgccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3361 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3421 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3481 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3541 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     3601 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     3661 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtgtc gcccgaccac
     3721 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3781 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
     3841 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3901 cgcaagcccg gtgcccctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     3961 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4021 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4081 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4141 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4201 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4261 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4321 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4381 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4441 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4501 ccctcagacg agtcggatct ccctttgggc cgcctcccyk yawsrmyamy gwckwmcwmg
     4561 rsrgctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4621 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4801 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     4861 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     4921 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     4981 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     5041 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     5101 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     5161 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     5221 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     5281 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     5341 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     5401 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5461 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5521 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5581 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5701 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6001 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6301 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7441 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7501 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7561 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7621 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7741 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     7981 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     8041 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     8101 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     8161 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     8221 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     8281 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     8341 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     8401 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     8461 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     8521 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     8581 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     8641 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     8701 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     8761 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     8821 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     8881 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     8941 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     9001 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     9061 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     9121 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     9181 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     9241 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     9301 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     9361 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     9421 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     9481 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     9541 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     9601 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     9661 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     9721 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     9781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9841 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     9901 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     9961 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10021 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10081 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10141 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    10201 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    10261 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    10321 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    10381 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    10441 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10681 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    10741 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    10801 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    10861 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    10921 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    10981 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11041 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11101 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    11161 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
    11221 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11281 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11341 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11401 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11461 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggg
//
