LOCUS       sequence-330428-       10645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of HA mtC11L34 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->HA-mtC11L34
FEATURES             Location/Qualifiers
     source          1..10645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             344..388
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    673..790
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        856..2034
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1087..2025
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1982..2002
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2051..2060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2120..2146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3132..3720
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3185..3205)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3603..3614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3723..3739)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3724..3740)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3830..4063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4088..4106)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4089..4106)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4396..4415)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4629..4648)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4748..4770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4808..4826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4894..4998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4999..5067,5068..5859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5217..5236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6030..6618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6519..6538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6772..6789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6859..6879)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6864..7193
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7044..7179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8327..8392
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8522..8542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8967..9101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9004..9023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9058..9077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9269..9902
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9949..10074
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(10571..10645,1..159)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
       61 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
      121 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
      181 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
      241 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
      301 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
      361 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
      421 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
      481 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
      541 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
      601 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
      661 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      721 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      781 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
      841 tatcgaattc cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      901 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      961 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     1021 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     1081 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     1141 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     1201 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     1261 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     1321 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     1381 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     1441 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1501 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1561 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1621 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1681 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1741 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1801 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1861 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1921 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1981 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagaattc
     2041 tcacgcggcc gccaccatgg cattgcccgt gaccgccctg ctgctgccac tggccttgtt
     2101 gctccacgcc gcgcggccat acccatacga tgttccagat tacgctgacg ccgttgtgac
     2161 ccaggaatcc gctctgacca cctctccagg cgaaaccgtg actctgactt gccgtagtag
     2221 caccggggct gtgaccacat ctaactatgc cagttgggtc caggaaaaac cggatcacct
     2281 gtttactggc ctgattggcg gcaccaacaa tcgcgcaccg ggtgtgcccg ctcgtttcag
     2341 cggttccctg attggggaca aggcagcact gactatcacc ggcgcccaga ccgaagatga
     2401 ggcgatctat ttttgcgccc tgtggtacag caaccattgg gtgttcgggg gaggcaccaa
     2461 actgacagtg ctgggcggag gaggaggttc aggaggagga ggtagcgggg gaggcggttc
     2521 cgggggaggc ggttctgatg tgcagctgca agaatccggg ccaggactgg ttgcgccttc
     2581 tcagagtctg tcaattacat gtactgttag tggctttagc ctgaccgact atggtgtgaa
     2641 ctgggttcgt cagagcccag gcaagggtct ggagtggctg ggagtgattt ggggggatgg
     2701 aatcacagac tacaatagcg cactgaaatc tcggctgagt gttaccaaag ataacagcaa
     2761 gtcccaggtc ttcctgaaga tgaacagcct gcaaagcggc gactccgctc gctattactg
     2821 cgttaccgga ctgtttgatt attgggggca ggggacaact ctgactgttt cctccaacgc
     2881 cgtgggccag gatacgcagg aggtgatcgt cgtgccccac agcttgccct tcaaggtcgt
     2941 ggttattagt gctatcctgg cgttggtggt gctgactatt atctccctta tcatacttat
     3001 catgctgtgg caaaaaaaac cccgataatt aattaaggcc gcaactccca cctgcaacat
     3061 gcgtgactga ctgaggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca
     3121 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3181 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3241 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3301 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3361 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3421 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3481 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3541 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3601 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3661 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3721 atcgataccg tcgacctcga gggaattaat tcgagctcgg tacctttaag accaatgact
     3781 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3841 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3901 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3961 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4021 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     4081 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     4141 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     4201 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     4261 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     4321 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     4381 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     4441 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     4501 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     4561 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     4621 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4681 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4741 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     4801 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4861 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4921 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4981 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5041 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5101 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5161 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5221 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5281 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5341 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5401 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5461 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5521 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5581 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5641 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5701 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5761 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5821 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5881 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5941 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6001 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6061 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6781 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6841 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6901 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6961 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7021 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7081 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7141 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     7201 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     7261 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     7321 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     7381 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     7441 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     7501 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     7561 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     7621 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     7681 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     7741 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     7801 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     7861 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     7921 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     7981 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     8041 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     8101 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     8161 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     8221 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     8281 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     8341 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     8401 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     8461 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     8521 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     8581 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     8641 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     8701 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     8761 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     8821 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     8881 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     8941 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9061 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     9121 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     9181 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     9241 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     9301 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     9361 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     9421 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     9481 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     9541 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     9601 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     9661 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     9721 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9901 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     9961 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    10021 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    10081 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    10141 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    10201 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    10261 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    10321 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    10381 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
    10441 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    10501 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    10561 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    10621 cagcgtcaat gacgctgacg gtaca
//
