LOCUS       sequence-330437-       20787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gentamycin resistant plasmid for Tn7-mediated chromosomal 
            integration of Photorhabdus luminescens luxCDABE operon with Pkan 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20787)
  AUTHORS   Matsumoto A, Schluter T, Melkonian K, Takeda A, Nakagami H, Mine A
  TITLE     A versatile Tn7 transposon-based bioluminescence tagging tool for 
            quantitative and spatial detection of bacteria in plants.
  JOURNAL   Plant Commun. 2021 Jul 20;3(1):100227. doi: 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10.
  PUBMED    35059625
REFERENCE   2  (bases 1 to 20787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2021-07-20"; volume: "3(1):100227. doi"; pages: " 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10"
COMMENT     Sequence Label: pBJ2
FEATURES             Location/Qualifiers
     source          1..20787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  26..191
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1037..1058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1073..1078,1079..1096,1097..1103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1111..1127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1135..1151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1169..1187
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1169..1186
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(7791..8669)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        8696..8980
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     8903..8922
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(15671..15688)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      15841..15927
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      16019..16046
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(16127..16147)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16127..16145)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16166..16182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16190..16206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16214..16220,16221..16238,16239..16244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16259..16280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16550..16555,16556..16572,16573..16578)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             16767..17300
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      18582..19353
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             19354..20016
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLVHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     20710..20732
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20725..20741
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20751..20770
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20751..20769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggaagaa atagcgcttt cagcctgtgg gcggacaaaa tagttgggaa ctgggagggg
       61 tggaaatgga gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt
      121 gtagcgtcgt aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca
      181 cttatctggt tggtcgacac tagtattacc ctgttatccc tagatttaaa tgatatcgga
      241 tcctagtaag ccacgtttta attaatcaga tccctcaata gcggccgcaa gcttaccacc
      301 agatttccat ctgggcaccg aaggtccact cgtcgctgtc gccacgaccg aagctgccgc
      361 cgttgaaatc agcaggaacg gctttgccga agttcgcgtt gttatcagcg ttaccggtgt
      421 agtcgtaacc ccatttctca tcccacttgg cgtaggttgc gaagacacga atagccgggc
      481 gtgaccagat gctgtcgcca gcctgccatt gttgtgcgag ggtaattttg tactgattgt
      541 tcttgtcgcc ggtgcgctgg gattcgacgt tgtcgtagcc gatttccatc acggtgctca
      601 tgattggcgt ccacttgtac atcgggcgaa taccgacggt ccaccacttg gtgccgttgt
      661 cgttatccca gttgatatcc tggtacatac ccacgtacat catgtcccag ttgtcgccca
      721 tggagatcgc accgtggtcg aggatacgca gcatgtgacc gttgttgttg atattgtagg
      781 caaatttttc gttatcaaat gcaacgccag aaccctgcga cagcccttta ccctgcgagg
      841 tcatcgagtc agtagcgtac tgaacaacaa acttgttaaa gcccttcagg acactctgag
      901 tatgttcagc agtgaataac cagccgtctt tcgatgcgcc atcaaccaga cgatagttat
      961 cacgcaagtt ggcacgaccg tagtcctggc acgacaggtt tcccgactgg aaagcgggca
     1021 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     1081 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     1141 acagctatga ccatgattac gccaagctat ttaggtgaca ctatagaata ctcaagctat
     1201 gcatcaagct tggtaccgag ctcggatccg aattcactcc cttgccatcg cgatgcatct
     1261 taaaatccat agagtatatc aactattaaa tgcttggttt aagcttaaag cacacccttt
     1321 ctgtgccctc gtattggcgc gacgtaaaat ctcaacaagt acaccaggat attgaccata
     1381 ttctctgctc attatcccga catcatcggt aacaataaat gccggataac tcgttgatga
     1441 cgcatccata taactcatca aacccggcat tccatcaggt acaggtttca atgtttcagg
     1501 atcaagtgct cgcgcatata cccacggcgg aacacgttta cgttgcattt catcctcaaa
     1561 gaaacaagtg ttgagttcaa cttgattaaa tatatcgcgg atttgactaa tattattgag
     1621 gttgaacgtg tcgaataaaa gatgattgaa atcatcgcgt tttagggact ctttttcata
     1681 acttttccag ccgccccccg ttatgatata aaggttttta tccccataaa atgagatatt
     1741 tttatctttc atatactggc agagcaaata aataaagtat ggtgagccga taagacaaat
     1801 atcttttcct tgatttttta ttcgctcaag gctattcaat gtcttaacaa aatctattcg
     1861 ttcttccatt acggtaaatg tcgtgggata taataattct accaagctca taacatattt
     1921 aaaccaaatg ttatgagcat taaatctatc tggccctaag ttgaccaact ctatttgatg
     1981 atcaaaccaa ctaccaacat atttcatgcc ataactcaca gagcctaaga gtctctcaat
     2041 acttagtctg ttacgcgcca cctgactttt taaaccactc gtgccgctgc tggtaaacca
     2101 actttcaatc tcgttctcct gagaagttaa taagcgagta aacttaaaaa ctgatgttgg
     2161 gaatacaggt atgtcatcaa tttccgtaat attgtcgtct actttgtgta cctgacagta
     2221 gtgacggtat tcttgacaat gtttatagtg attacgaaat gcatcaagaa caaatttgtt
     2281 tctgattttt tcctgttcat cgtaagacca agctaatgga tcgctggaaa aaatcaaatc
     2341 atcaatttct gagcttgcta tgatctcttg tttatcaaca tatgaagtca tacctgtttt
     2401 acccctccaa ataatttaga gaatatggca gcgttgctgc catattcttt tactacatgt
     2461 ggtacttttt aatattatca ttaacaatat tgattacgtt tttttggtgc atcaagtcat
     2521 tcattggttc aaaagataac aatacacttt ttgcaccgca cttttcaatt gccagcttag
     2581 ccgcagctat acattccgta taatctccga cagcgttttc tgtgattatc tcttcaagtt
     2641 tattttcgag attttcatta ggatacattt caagaacata atcacggata aatgcacgcg
     2701 tctcttgttt agctttgtga ctatcttcgt tatagttaac caataccatt aactgatgat
     2761 ctatcgctga taagtcaata ccatatttat cagcaacggc tttatacctt tcagcatatt
     2821 catatctaac atcattggag tcatcccact taaagatgag aggaatgcct tttttagccg
     2881 cccactcaac aatatgatga ctggttgctg tgacatatct ccgaggcccg ccttgggtat
     2941 aagcgtgggg gtttaccgat attttaggga aactataaaa atcattatcg ggattacaat
     3001 agcctgttgt taaagcatcg ttaatgattt cataacactc ttcaaatagt tgctgttgat
     3061 attcaacagg gcgattaaaa agacgcattt catctttttt ttcacaatca ctaaacccta
     3121 aaataaatct cccttcgctt aattgatcca ataagcaagc ctcctccgct attcggacag
     3181 gatgatgagt tgtaatgatg tgattcaatg agccaatttt aattttttct gttaaaccga
     3241 gcaaaaaacc agaaacagtc agaggagcac cgacaacacc attacctgaa aaatgatttt
     3301 catacaccaa aatctgttca aaattcaact tatcaacata ctctgttatt tcctgcatgc
     3361 gaactatact ttgttcttga acagttgttg aattgatgaa gttaaggaag aacaatccaa
     3421 atttcatttc tttttcctta gctactataa tagcgaacgt tgtttttctt taagaaacgg
     3481 catgacatca gactggaaga gcttcatgga agcaataatt tcgtctactg ttccattcgc
     3541 ttcaaaccca caacagatat ttgatattcc cgtggcatca atgtcttttt gaattatgtc
     3601 aatgcattcc tgcggggttc ccacgggatt gatttcgtaa ctgtaatcaa tgcggcgatt
     3661 agtatctcta tgtcccttta atacaaagtc acgccactgc cctttattga aatcataacc
     3721 tcttgttttg tctgaatcat caaaaatggt cgtggcattc acataagaat cataccaatg
     3781 ccctagaaaa tcccgacaaa tctcttgcgc tttatttgag tcataattta cagatgttat
     3841 atatgataag caatggtcga tattatgaat atcgtgccca tattcttgag ccacctcgtt
     3901 ataaagctca agctgtgctt tcttttcgtt agtatttata atccaactta atatcatcgg
     3961 taaaccaaat tgagcggccc attcagttgt cgaggctgat tcagcaacca cataaacagg
     4021 ggctccacct ttactatatg ctgtcgggtt tacttttacc ttatggaact tgatatgttc
     4081 attatcagct tccatatatc cctctgtcat gccatttttt atcaacccgt accagcactc
     4141 cgttaaagcg cgactgttat tcatatccgt gccaaatacg cgaaagtctt tattgtaaag
     4201 cccccgacaa ataccaaacc gaaatcgtcc ttttgacatt tgatccaata aattcacatc
     4261 ttcaagttgg cgcactggat gagcggtggg aagaacaata gccgcagtcc ctacattcaa
     4321 ttttttggtt gcaccaagta aatatgcagc agcgacataa gggttaccaa gcaaaccaaa
     4381 ctccgtgaaa tgatgctcca gtaaccatac agtatcaaaa ccacactcct cagaaatacg
     4441 acctaattta accaaacgtt tcattacttc tgtttgagaa aattgggggg gttggtatgt
     4501 aagcaaaaag tttccaaatt tcatagagag cccttatatt tgagtgatag aattaactat
     4561 acccgttatc tttcaagttg cttctttgtt ggctgcactc actcaccccg gtcacatagt
     4621 ttgctatgct cccgggattc gctcccttgc cgtcgcgatc catcttgaaa tctatagggt
     4681 aatatctatc ccaataggtt ttaagacagc gaaatcgctt gattttcaat ctcaattttc
     4741 attcgacgtt cattgactgt cgcaatagtt agatgttcga atgatggttc aataatatca
     4801 acatcaatat ccagacgatc attatccatc gcgatagcag ccttcgtaac cgattgataa
     4861 aaattgcgca ggaccactaa gttttcaccc aagtcatgcg aactccctag caaagaatat
     4921 atcttgcatc gattactacg aatatttgat aacaatgtga taacttcatc ttgctttacc
     4981 caattgtcgt tatttgcagt aaaagcaata aacggtatat caagatacat catgctattg
     5041 attgtagaag ttaaatcttc ccagccaaaa tcaaggcaat ctctcgcaaa gacttcagca
     5101 cccaatttat ggccttcaaa atccaaatta ttcggcaatt cattaatggg taaactgaga
     5161 taatcaaatc ctaaagctct ttcaagcgta tatcttaagt taacaacacc gactgcggtg
     5221 attaaaaatg aaacattaat ttcagataga cttgcataaa ctatccgtgc agataagctt
     5281 gaagccaaaa taccacggtt atttattttt cgtgtattta accaatcaac cacggctaat
     5341 aggctctgtt ttcctataga cattgtaaat tcatcaattg tccctgaact caacccaacg
     5401 tggtgaagtg aatcatagcg aatcacatga aacccattcc gcgataaata ttccgctaaa
     5461 ccagcaaaat gatccatcct tcgggcaaaa cccgacgcaa taataatggt attctttctc
     5521 tttgggctgt tttcttctgg cagcgtttcc caaacatgaa tttttttatt tccttcaaca
     5581 caaagaacat ggtcgatggt tttatatttg gatttatttt ccatactttt tacctattac
     5641 gggacaaata caaggaactt atcttcttcc aggaatcgag tctgttctat ttcaaccgca
     5701 acatccttag cagtataatg agatggcctc tcatgagaaa tatatgtcac taatcgttgt
     5761 aacggcctca ttccgtcatg agatccacca actcgaaata tattattcat tcctgcttct
     5821 acaatccttt ccgcacctct taatgccaac gcatctcgat acttaaatgc ggattcccaa
     5881 ggaaaaatag atatggtttg tgtcttattt ttttgaacat aaggcaatac ttgctcaata
     5941 ttatcgacgt gatgaagata cacacatctg ccaagtggtt gattaaattc cacacccgca
     6001 tttgactcaa taatcatcca acgttgatga acatccacct ctacttttaa tccagcaaat
     6061 aagctctctt tttggactaa agaataggcc gccttttcat caaaatcttt tttggcgttt
     6121 ggtaatatat gcgcatatag attaagtttt tctatcaacg ctaacttaaa ttcctcatac
     6181 tgatttccca tgtaatatat gttttgggca gaaaaacaag cgcgctgatc gtaaaaacaa
     6241 atatcatgag ccgcaccggt agctgctgac gttaaatcaa ctggattatc aataatgcaa
     6301 aaactctttt tagagccaaa tttaatcacg tcagcatagg gtggtgcatg ttctacagcc
     6361 caattaatcg catcttcccc tccccaagcg acaataacat ccatatgttg cataatttct
     6421 tttgcgagtg atgtatcacc ttggtgtggc caatatacaa cagacaaaga gcgcgttatc
     6481 ggatgattag ggtctacatc gataaagctt aacgctaatg cattagcggt aaagggatcg
     6541 gttgacgatg tttttataat acactgattc ttggttaaaa ttgcgcgtaa tatagacatg
     6601 atcccagata atggaacatt acctgccaac agatgtatag acttaccttt cggaaaagcc
     6661 ttaatataac tttcatcctg aggtagccat tcatccatga tatggcgaga accaagttca
     6721 ttttctacaa catcataaag gccaccttta gaacataaaa tcatagatat ccagttggcc
     6781 tctaacttgg ccattgcttc tgaatatccc atatattttt ttaaatcacg aatgtatgtc
     6841 ctgcgccttg aatattcttc atttttccat ctttgcccta ccgtatagag aaaattgaca
     6901 atattatgca accgtaattt attattttca ttataatcaa taatgttttt tacatgagaa
     6961 ttattcaata ttggcaggta aacactatta tcaccaaaat taatggattg cactaaatca
     7021 tcactttcgg gaaaaatttc aacctggccg ttaataatga atgaaatttt tttagtcata
     7081 ttagccatcc atttaatggg gtaacttcaa cttaagttat tgtttaatca tcttgcaaac
     7141 tggttgtacc gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc
     7201 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     7261 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     7321 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     7381 ttgtccagat agcccagtag ctgacattca tccgcaccta ggaggcgcgc cacggccgcg
     7441 tcgaccccac gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt
     7501 gctttagcca taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt
     7561 ttacaactat tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag
     7621 tttaagactt tattgtccgc ccacaggcca cgatgcgtcc ggcgtagagg atctgctcat
     7681 gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa tggacgaagc agggattctg
     7741 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac
     7801 ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc
     7861 ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga
     7921 ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt
     7981 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
     8041 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
     8101 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc
     8161 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
     8221 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
     8281 aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc
     8341 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
     8401 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
     8461 tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
     8521 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
     8581 ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc
     8641 agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa
     8701 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
     8761 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
     8821 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
     8881 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     8941 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc
     9001 gaattcgagc tcggtaccca agtcttaaac tagacagaat agttgtaaac tgaaatcagt
     9061 ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt
     9121 ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga
     9181 ctatattcca tggctaacag tacaagaagt tccttcttca ggtcgttccc accgtattta
     9241 ttctcataag acgggacgag tccatcattt gctatctgac ttagagcttg ctgtttttct
     9301 cagtcttgag tgggagagca gcgtgctaga tatacgcgag cagttcccct tattacctag
     9361 tgataccagg cagattgcaa tagatagtgg tattaagcat cctgttattc gtggtgtaga
     9421 tcaggttatg tctactgatt ttttagtgga ctgcaaagat ggtccttttg agcagtttgc
     9481 tattcaagtc aaacctgcag cagccttaca agacgagcgt accttagaaa aactagaact
     9541 agagcgtcgc tattggcagc aaaagcaaat tccttggttc atttttactg ataaagaaat
     9601 aaatcccgta gtaaaagaaa atattgaatg gctttattca gtgaaaacag aagaagtttc
     9661 tgcggagctt ttagcacaac tatccccatt ggcccatatc ctgcaagaaa aaggagatga
     9721 aaacattatc aatgtctgta agcaggttga tattgcttat gatttggagt taggcaaaac
     9781 attgagtgag atacgagcct taaccgcaaa tggttttatt aagttcaata tttataagtc
     9841 tttcagggca aataagtgtg cagatctctg tattagccaa gtagtgaata tggaggagtt
     9901 gcgctatgtg gcaaattaat gaggttgtgc tatttgataa tgatccgtat cgcattttgg
     9961 ctatagagga tggccaagtt gtctggatgc aaataagcgc tgataaagga gttccacaag
    10021 ctagggctga gttgttgcta atgcagtatt tagatgaagg ccgcttagtt agaactgatg
    10081 acccttatgt acatcttgat ttagaagagc cgtctgtaga ttctgtcagc ttccagaagc
    10141 gcgaggagga ttatcgaaaa attcttccta ttattaatag taaggatcgt ttcgacccta
    10201 aagtcagaag cgaactcgtt gagcatgtgg tccaagaaca taaggttact aaggctacag
    10261 tttataagtt gttacgccgt tactggcagc gtggtcaaac gcctaatgca ttaattcctg
    10321 actacaaaaa cagcggtgca ccaggggaaa gacgttcagc gacaggaaca gcaaagattg
    10381 gccgagccag agaatatggt aagggtgaag gaaccaaggt aacgcccgag attgaacgcc
    10441 tttttaggtt gaccatagaa aagcacctgt taaatcaaaa aggtacaaag accaccgttg
    10501 cctatagacg atttgtggac ttgtttgctc agtattttcc tcgcattccc caagaggatt
    10561 acccaacact acgtcagttt cgttattttt atgatcgaga ataccctaaa gctcagcgct
    10621 taaagtctag agttaaagca ggggtatata aaaaagacgt acgaccctta agtagtacag
    10681 ccacttctca ggcgttaggc cctgggagtc gttatgagat tgatgccacg attgctgata
    10741 tttatttagt ggatcatcat gatcgccaaa aaatcatagg aagaccaacg ctttacattg
    10801 tgattgatgt gtttagtcgg atgatcacgg gcttttatat cggctttgaa aatccgtctt
    10861 atgtggtggc gatgcaggct tttgtaaatg cttgctctga caaaacggcc atttgtgccc
    10921 agcatgatat tgagattagt agctcagact ggccgtgtgt aggtttgcca gatgtgttgc
    10981 tagcggaccg tggcgaatta atgagtcatc aggtcgaagc cttagtttct agttttaatg
    11041 tgcgagtgga aagtgctcca cctagacgtg gcgatgctaa aggcatagtg gaaagcactt
    11101 ttagaacact acaagccgag tttaagtcct ttgcacctgg cattgtagag ggcagtcgga
    11161 tcaaaagcca tggtgaaaca gactataggt tagatgcatc tctgtcggta tttgagttca
    11221 cacaaattat tttgcgtacg atcttattca gaaataacca tctggtgatg gataaatacg
    11281 atcgagatgc tgattttcct acagatttac cgtctattcc tgtccagcta tggcaatggg
    11341 gtatgcagca tcgtacaggt agtttaaggg ctgtggagca agagcagttg cgagtagcgt
    11401 tactgcctcg ccgaaaggtc tctatttctt catttggcgt taatttgtgg ggtttgtatt
    11461 actcggggtc agagattctg cgtgagggtt ggttgcagcg gagcactgat atagctagac
    11521 ctcaacattt agaagcggct tatgacccag tgctggttga tacgatttat ttgtttccgc
    11581 aagttggcag ccgtgtattt tggcgctgta atctgacgga acgtagtcgg cagtttaaag
    11641 gtctctcatt ttgggaggtt tgggatatac aagcacaaga aaaacacaat aaagccaatg
    11701 cgaagcagga tgagttaact aaacgcaggg agcttgaggc gtttattcag caaaccattc
    11761 agaaagcgaa taagttaacg cccagtacta ctgagcccaa atcaacacgc attaagcaga
    11821 ttaaaactaa taaaaaagaa gccgtgacct cggagcgtaa aaaacgtgcg gagcatttga
    11881 agccaagctc ttcaggtgat gaggctaaag ttattccttt caacgcagtg gaagcggatg
    11941 atcaagaaga ttacagccta cccacatacg tgcctgaatt atttcaggat ccaccagaaa
    12001 aggatgagtc atgagtgcta cccggattca agcagtttat cgtgatacgg gggtagaggc
    12061 ttatcgtgat aatcctttta tcgaggcctt accaccatta caagagtcag tgaatagtgc
    12121 tgcatcactg aaatcctctt tacagcttac ttcctctgac ttgcaaaagt cccgtgttat
    12181 cagagctcat accatttgtc gtattccaga tgactatttt cagccattag gtacgcattt
    12241 gctactaagt gagcgtattt cggtcatgat tcgaggtggc tacgtaggca gaaatcctaa
    12301 aacaggagat ttacaaaagc atttacaaaa tggttatgag cgtgttcaaa cgggagagtt
    12361 ggagacattt cgctttgagg aggcacgatc tacggcacaa agcttattgt taattggttg
    12421 ttctggtagt gggaagacga cctctcttca tcgtattcta gccacgtatc ctcaggtgat
    12481 ttaccatcgt gaactcaatg tagagcaggt ggtgtatttg aaaatagact gctcgcataa
    12541 tggttcgcta aaagaaatct gcttgaattt tttcagagcg ttggatcgag ccttgggctc
    12601 gaactatgag cgtcgttatg gcttaaaacg tcatggtata gaaaccatgt tggctttgat
    12661 gtcgcaaata gccaatgcac atgctttagg gttgttggtt attgatgaaa ttcagcattt
    12721 aagccgctct cgttcgggtg gatctcaaga gatgctgaac ttttttgtga cgatggtgaa
    12781 tattattggc gtaccagtga tgttgattgg tacccctaaa gcacgagaga tttttgaggc
    12841 tgatttgcgg tctgcacgta gaggggcagg gtttggagct atattctggg atcctataca
    12901 acaaacgcaa cgtggaaagc ccaatcaaga gtggatcgct tttacggata atctttggca
    12961 attacagctt ttacaacgca aagatgcgct gttatcggat gaggtccgtg atgtgtggta
    13021 tgagctaagc caaggagtga tggacattgt agtaaaactt tttgtactcg ctcagctccg
    13081 tgcgctagct ttaggcaatg agcgtattac cgctggttta ttgcggcaag tgtatcaaga
    13141 tgagttaaag cctgtgcacc ccatgctaga ggcattacgc tcgggtatcc cagaacgcat
    13201 tgctcgttat tctgatctag tcgttcccga gattgataaa cggttaatcc aacttcagct
    13261 agatatcgca gcgatacaag aacaaacacc agaagaaaaa gcccttcaag agttagatac
    13321 cgaagatcag cgtcatttat atctgatgct gaaagaggat tacgattcaa gcctgttaat
    13381 tcccactatt aaaaaagcgt ttagccagaa tccaacgatg acaagacaaa agttactgcc
    13441 tcttgttttg cagtggttga tggaaggcga aacggtagtg tcagaactag aaaagccctc
    13501 caagagtaaa aaggtttcgg ctataaaggt agtcaagccc agcgactggg atagcttgcc
    13561 tgatacggat ttacgttata tctattcaca acgccaacct gaaaaaacca tgcatgaacg
    13621 gttaaaaggg aaaggggtaa tagtggatat ggcgagctta tttaaacaag caggttagcc
    13681 atgagaaact ttcctgttcc gtactcgaat gagctgattt atagcactat tgcacgggca
    13741 ggcgtttatc aagggattgt tagtcctaag cagctgttgg atgaggtgta tggcaaccgc
    13801 aaggtggtcg ctaccttagg tctgccctcg catttaggtg tgatagcaag acatctacat
    13861 caaacaggac gttacgctgt tcagcagctt atttatgagc ataccttatt ccctttatat
    13921 gctccgtttg taggcaagga gcgccgagac gaagctattc ggttaatgga gtaccaagcg
    13981 caaggtgcgg tgcatttaat gctaggagtc gctgcttcta gagttaagag cgataaccgc
    14041 tttagatact gccctgattg cgttgctctt cagctaaata ggtatgggga agccttttgg
    14101 caacgagatt ggtatttgcc cgctttgcca tattgtccaa aacacggtgc tttagtcttc
    14161 tttgatagag ctgtagatga tcaccgacat caattttggg ctttgggtca tactgagctg
    14221 ctttcagact accccaaaga ctccctatct caattaacag cactagctgc ttatatagcc
    14281 cctctgttag atgctccacg agcgcaagag ctttccccaa gccttgagca gtggacgctg
    14341 ttttatcagc gcttagcgca ggatctaggg ctaaccaaaa gcaagcacat tcgtcatgac
    14401 ttggtggcgg agagagtgag gcagactttt agtgatgagg cactagagaa actggattta
    14461 aagttggcag agaacaagga cacgtgttgg ctgaaaagta tattccgtaa gcatagaaaa
    14521 gcctttagtt atttacagca tagtattgtg tggcaagcct tattgccaaa actaacggtt
    14581 atagaagcgc tacagcaggc aagtgctctt actgagcact ctataacgac aagacctgtt
    14641 agccagtctg tgcaacctaa ctctgaagat ttatctgtta agcataaaga ctggcagcaa
    14701 ctagtgcata aataccaagg aattaaggcg gcaagacagt ctttagaggg tggggtgcta
    14761 tacgcttggc tttaccgaca tgacagggat tggctagttc actggaatca acagcatcaa
    14821 caagagcgtc tggcacccgc ccctagagtt gattggaacc aaagagatcg aattgctgta
    14881 cgacaactat taagaatcat aaagcgtcta gatagtagcc ttgatcaccc aagagcgaca
    14941 tcgagctggc tgttaaagca aactcctaac ggaacctctc ttgcaaaaaa tctacagaaa
    15001 ctgcctttgg tagcgctttg cttaaagcgt tactcagaga gtgtggaaga ttatcaaatt
    15061 agacggatta gccaagcttt tattaagctt aaacaggaag atgttgagct taggcgctgg
    15121 cgattattaa gaagtgcaac gttatctaaa gagcggataa ctgaggaagc acaaagattc
    15181 ttggaaatgg tttatgggga agagtgagtg gttaggctag ctacatttaa tgacaatgtg
    15241 caggttgtac atattggtca tttattccgt aactcgggtc ataaggagtg gcgtattttt
    15301 gtttggttta atccaatgca agaacggaaa tggactcgat ttactcattt gcctttatta
    15361 agtcgagcta aggtggttaa cagtacaaca aagcaaataa ataaggcgga tcgtgtgatt
    15421 gagtttgaag catcggatct tcaacgagcc aaaataatcg attttcctaa tctctcgtcc
    15481 tttgcttccg tacgcaacaa ggatggagcg cagagttcat ttatttacga agctgaaaca
    15541 ccatatagca agactcgtta tcacatccca cagttagagc tagctcggtc attattttta
    15601 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg ctgttttggc ggatgagaga
    15661 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
    15721 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
    15781 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
    15841 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
    15901 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
    15961 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
    16021 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
    16081 aatacattca aatatgtatc cgcggcccgg tacccagctt ttgttccctt tagtgagggt
    16141 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    16201 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    16261 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    16321 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    16381 ggcgcatgca taaaaactgt tgtaattcat taagcattct gccgacatgg aagccatcac
    16441 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
    16501 atttgcccat ggacgcacac cgtggaaacg gatgaaggca cgaacccagt tgacataagc
    16561 ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
    16621 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
    16681 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
    16741 atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg
    16801 gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc
    16861 tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg
    16921 gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc
    16981 gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg
    17041 cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg
    17101 cagtctccgg agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc
    17161 atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc
    17221 ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg
    17281 acccaagtac cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt
    17341 tgttcggtaa attgtcacaa cgccgcaggt ggcacttttc ggggaaatgt gcgcgcccgc
    17401 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct
    17461 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc
    17521 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggtc tcgggccgtc tcttgggctt
    17581 gatcggcctt cttgcgcatc tcacgcgctc ctgcggcggc ctgtagggca ggctcatacc
    17641 cctgccgaac cgcttttgtc agccggtcgg ccacggcttc cggcgtctca acgcgctttg
    17701 agattcccag cttttcggcc aatccctgcg gtgcataggc gcgtggctcg accgcttgcg
    17761 ggctgatggt gacgtggccc actggtggcc gctccagggc ctcgtagaac gcctgaatgc
    17821 gcgtgtgacg tgccttgctg ccctcgatgc cccgttgcag ccctagatcg gccacagcgg
    17881 ccgcaaacgt ggtctggtcg cgggtcatct gcgctttgtt gccgatgaac tccttggccg
    17941 acagcctgcc gtcctgcgtc agcggcacca cgaacgcggt catgtgcggg ctggtttcgt
    18001 cacggtggat gctggccgtc acgatgcgat ccgccccgta cttgtccgcc agccacttgt
    18061 gcgccttctc gaagaacgcc gcctgctgtt cttggctggc cgacttccac cattccgggc
    18121 tggccgtcat gacgtactcg accgccaaca cagcgtcctt gcgccgcttc tctggcagca
    18181 actcgcgcag tcggcccatc gcttcatcgg tgctgctggc cgcccagtgc tcgttctctg
    18241 gcgtcctgct ggcgtcagcg ttgggcgtct cgcgctcgcg gtaggcgtgc ttgagactgg
    18301 ccgccacgtt gcccattttc gccagcttct tgcatcgcat gatcgcgtat gccgccatgc
    18361 ctgcccctcc cttttggtgt ccaaccggct cgacgggggc agcgcaaggc ggtgcctccg
    18421 gcgggccact caatgcttga gtatactcac tagactttgc ttcgcaaagt cgtgaccgcc
    18481 tacggcggct gcggcgccct acgggcttgc tctccgggct tcgccctgcg cggtcgctgc
    18541 gctcccttgc cagcccgtgg atatgtggac gatggccgcg agcggccacc ggctggctcg
    18601 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
    18661 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
    18721 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
    18781 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
    18841 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
    18901 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
    18961 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
    19021 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
    19081 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
    19141 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
    19201 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
    19261 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
    19321 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
    19381 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
    19441 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
    19501 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
    19561 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
    19621 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
    19681 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
    19741 cacgacgacc aggacgaatc gctgttggtg catggcgacc tgcgccgcat cccgaccctg
    19801 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
    19861 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc
    19921 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
    19981 gagccgccga cacgggtcac gctgccgcgc cggtagcact tgggttgcgc agcaacccgt
    20041 aagtgcgctg ttccagacta tcggctgtag ccgcctcgcc gccctatacc ttgtctgcct
    20101 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
    20161 cacctcgcta acggattcac cgtttttatc aggctctggg aggcagaata aatgatcata
    20221 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
    20281 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
    20341 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
    20401 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
    20461 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtacggc ctattggtta
    20521 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    20581 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    20641 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    20701 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    20761 cactataggg cgaattggtc gaggggg
//
