LOCUS       sequence-330438-       11601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 
            with IFP NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP
FEATURES             Location/Qualifiers
     source          1..11601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    158..275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        341..1519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          572..1510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1467..1487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1536..1545
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1593..1622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2448..2504
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2517..2537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2541..2561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2574..3536
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     misc_feature    3573..4161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3626..3646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4044..4055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4164..4180)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4165..4181)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4271..4504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4529..4547)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4530..4547)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4837..4856)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5070..5089)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5189..5211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5249..5267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5335..5439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5440..5508,5509..6300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5658..5677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6471..7059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6960..6979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7213..7230
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7300..7320)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7305..7634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7485..7620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8768..8833
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8963..8983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9408..9542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9445..9464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9499..9518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9710..10343
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10390..10515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11012..11245
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11430..11474
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
       61 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      121 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      241 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      301 atccagtttg gatcgataag cttgatatcg aattccgtga ggctccggtg cccgtcagtg
      361 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      421 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      481 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      541 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      601 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      661 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      721 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      781 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      841 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      901 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
      961 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1021 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1081 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1141 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1201 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1261 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1321 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1381 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1441 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1501 tccatttcag gtgtcgtgag aattctcacg cggccgccac catgggcgtt aaagtccttt
     1561 tcgccctgat ctgcattgcc gtcgcagagg ccgagcagaa actcattagt gaggaagatc
     1621 tgatggccca ggttcagctc gtagagtcag gaggccgcct tgtccaggcg ggggatagtc
     1681 tcaggcttag ctgcgctgca agcggccgga ctttcagcac gtccgctatg gcttggttta
     1741 ggcaggcccc cgggagagag cgcgagttcg tagcagccat tacgtggacc gtgggaaaca
     1801 ccatcttggg tgacagtgtg aaagggcgat tcaccatctc aagggatcgc gctaagaata
     1861 ctgttgacct gcaaatggac aatcttgagc ccgaagacac cgccgtatac tattgtagcg
     1921 ctagaagtcg gggctacgtg ttgtccgtcc tcaggtctgt cgactcctac gattactggg
     1981 ggcagggcac gcaagtcaca gtgagcggag gaggtggttc agggggcggg gggagcggtg
     2041 gcggtggcag catggcacag gtccagttgg tagaatctgg cggcggcctc gttcaagcgg
     2101 ggggctcact tcgactttca tgtgcggcca gcgggcgcac attttcaaac tacgctatgg
     2161 gctggtttcg acaagccccc ggaaaagaga gagagtttgt ggctgccata agctggactg
     2221 gggtgtccac atactacgct gattccgtaa agggcaggtt cacgataagt agagataatg
     2281 acaaaaatac cgtttacgtc cagatgaact ccctgatccc ggaggacact gccatctact
     2341 attgcgctgc cgttcgggcc cggagctttt ccgacactta ctcacgcgtc aacgagtacg
     2401 attactgggg gcaggggacc caggtcacag ttgccagcgg cagcggcgcc acaaacttct
     2461 ctctgctaaa gcaagcaggt gatgttgaag aaaaccccgg gcctcatatg gtggatccaa
     2521 aaaagaagag aaaggtagat ccaaagaaga aaaggaaggt tggcagcggc tccgctcggg
     2581 accctcaacc tttcttccct cctctgtatc tcggaggacc agagatcact actgagaact
     2641 gcgagcggga acctatacat ataccaggtt ctattcaacc tcatggagca ctgctcacag
     2701 ccgatggaca ttctggcgag gtgctgcaag tttcactcaa cgcagccact ttcctgggcc
     2761 aggaaccaac tgttctgcgg ggacagaccc tggcagccct gctgccagac cagtggcctg
     2821 ctctgcagac tgccctgcct ccaggctgcc aggacgccct gcagtatcgc gccaccctgg
     2881 attggcccgc cgccggacac ctgagcctga ccgtgcatag ggtggccgag ctgctgattc
     2941 tggaattcga gcctactgag gcatgggact ctattggacc ccacgctctg agaaatgcaa
     3001 tgttcgctct ggagagtgct cctaatctcc gggcactggc tgaagtcgca acccaaacag
     3061 tccgggaact gtcaggtttc gaccgggtga tgctgtacaa gtttgcacca gacgcaacag
     3121 gagaggttat tgccgaagca aggcgcgagg gcatgcaggc ttacctcggg cataggtttc
     3181 ccgcatccac cacccctgca caagctaggg ccctctacac aagacacctg ctccggctga
     3241 ccgcagacac cagggctgca gcagtgcccc tcgaccccgt gctgaatccc cagacaaatg
     3301 ctcctacacc tctgggcgga gctgtcctca gagctacatc cccaatgcac atgcagtacc
     3361 tgaggaatat gggagtgggc tcctccctga gcgtcagcgt cgtggtcggc ggccagctgt
     3421 ggggactgat tgtctgccac catcagacac cctacgtgct gccacccgat ctgcggacca
     3481 ccctggagta tctggggagg ctcctgtccc tgcaggtgca gaggaaagaa gcctgaccgc
     3541 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     3601 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3661 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     3721 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     3781 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     3841 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     3901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     3961 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4021 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4081 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4141 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
     4201 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4261 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     4501 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     4561 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     4621 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     4681 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     4741 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     4801 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     4861 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     4921 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     4981 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     5041 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5101 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5161 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5221 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5281 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5641 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7081 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7141 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7201 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7261 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
     7321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7381 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7441 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7501 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7561 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7621 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
     7681 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     7741 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     7801 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     7861 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     7921 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
     7981 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
     8041 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
     8101 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
     8161 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
     8221 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
     8281 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
     8341 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
     8401 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
     8461 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
     8521 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
     8581 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
     8641 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
     8701 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     8761 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     8821 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     8881 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     8941 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     9001 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     9061 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     9121 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     9181 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     9241 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     9301 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     9361 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     9421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9541 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9601 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9661 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     9721 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     9781 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     9841 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     9901 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     9961 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    10021 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    10081 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    10141 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10201 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10261 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10321 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    10381 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    10441 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    10501 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    10561 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    10621 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    10681 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    10741 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    10801 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
    10861 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    10921 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    10981 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    11041 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    11101 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    11161 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    11221 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    11281 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    11341 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    11401 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    11461 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    11521 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    11581 tgaatagagt taggcaggga t
//
