LOCUS       sequence-330441-       21290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A kanamycin resistant plasmid for Tn7-mediated chromosomal 
            integration of Photorhabdus luminescens luxCDABE operon with Pfrr 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21290)
  AUTHORS   Matsumoto A, Schluter T, Melkonian K, Takeda A, Nakagami H, Mine A
  TITLE     A versatile Tn7 transposon-based bioluminescence tagging tool for 
            quantitative and spatial detection of bacteria in plants.
  JOURNAL   Plant Commun. 2021 Jul 20;3(1):100227. doi: 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10.
  PUBMED    35059625
REFERENCE   2  (bases 1 to 21290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2021-07-20"; volume: "3(1):100227. doi"; pages: " 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10"
COMMENT     Sequence Label: pBJ3
FEATURES             Location/Qualifiers
     source          1..21290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(512..531)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1122..1141
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1790..1810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(1891..1918)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2010..2096)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2249..2266
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(8957..9241)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(9015..9034)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             9268..10146
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        complement(16794..16812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(16795..16812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(16830..16846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16854..16870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16878..16884,16885..16902,16903..16908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16923..16944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     mobile_element  17790..17955
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(18032..18048)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18032..18048)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(18080..18099)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18081..18099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18109..18125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18118..18140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(18834..19496)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      19497..20268
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccgcggata catatttgaa tgtatttaga
     1861 aaaataaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     1921 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     1981 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     2041 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     2101 ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt
     2161 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt
     2221 ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc
     2281 tcatccgcca aaacagccaa gcttgcatgc ctgcaggtcg actctagagg atcccctaaa
     2341 aataatgacc gagctagctc taactgtggg atgtgataac gagtcttgct atatggtgtt
     2401 tcagcttcgt aaataaatga actctgcgct ccatccttgt tgcgtacgga agcaaaggac
     2461 gagagattag gaaaatcgat tattttggct cgttgaagat ccgatgcttc aaactcaatc
     2521 acacgatccg ccttatttat ttgctttgtt gtactgttaa ccaccttagc tcgacttaat
     2581 aaaggcaaat gagtaaatcg agtccatttc cgttcttgca ttggattaaa ccaaacaaaa
     2641 atacgccact ccttatgacc cgagttacgg aataaatgac caatatgtac aacctgcaca
     2701 ttgtcattaa atgtagctag cctaaccact cactcttccc cataaaccat ttccaagaat
     2761 ctttgtgctt cctcagttat ccgctcttta gataacgttg cacttcttaa taatcgccag
     2821 cgcctaagct caacatcttc ctgtttaagc ttaataaaag cttggctaat ccgtctaatt
     2881 tgataatctt ccacactctc tgagtaacgc tttaagcaaa gcgctaccaa aggcagtttc
     2941 tgtagatttt ttgcaagaga ggttccgtta ggagtttgct ttaacagcca gctcgatgtc
     3001 gctcttgggt gatcaaggct actatctaga cgctttatga ttcttaatag ttgtcgtaca
     3061 gcaattcgat ctctttggtt ccaatcaact ctaggggcgg gtgccagacg ctcttgttga
     3121 tgctgttgat tccagtgaac tagccaatcc ctgtcatgtc ggtaaagcca agcgtatagc
     3181 accccaccct ctaaagactg tcttgccgcc ttaattcctt ggtatttatg cactagttgc
     3241 tgccagtctt tatgcttaac agataaatct tcagagttag gttgcacaga ctggctaaca
     3301 ggtcttgtcg ttatagagtg ctcagtaaga gcacttgcct gctgtagcgc ttctataacc
     3361 gttagttttg gcaataaggc ttgccacaca atactatgct gtaaataact aaaggctttt
     3421 ctatgcttac ggaatatact tttcagccaa cacgtgtcct tgttctctgc caactttaaa
     3481 tccagtttct ctagtgcctc atcactaaaa gtctgcctca ctctctccgc caccaagtca
     3541 tgacgaatgt gcttgctttt ggttagccct agatcctgcg ctaagcgctg ataaaacagc
     3601 gtccactgct caaggcttgg ggaaagctct tgcgctcgtg gagcatctaa cagaggggct
     3661 atataagcag ctagtgctgt taattgagat agggagtctt tggggtagtc tgaaagcagc
     3721 tcagtatgac ccaaagccca aaattgatgt cggtgatcat ctacagctct atcaaagaag
     3781 actaaagcac cgtgttttgg acaatatggc aaagcgggca aataccaatc tcgttgccaa
     3841 aaggcttccc catacctatt tagctgaaga gcaacgcaat cagggcagta tctaaagcgg
     3901 ttatcgctct taactctaga agcagcgact cctagcatta aatgcaccgc accttgcgct
     3961 tggtactcca ttaaccgaat agcttcgtct cggcgctcct tgcctacaaa cggagcatat
     4021 aaagggaata aggtatgctc ataaataagc tgctgaacag cgtaacgtcc tgtttgatgt
     4081 agatgtcttg ctatcacacc taaatgcgag ggcagaccta aggtagcgac caccttgcgg
     4141 ttgccataca cctcatccaa cagctgctta ggactaacaa tcccttgata aacgcctgcc
     4201 cgtgcaatag tgctataaat cagctcattc gagtacggaa caggaaagtt tctcatggct
     4261 aacctgcttg tttaaataag ctcgccatat ccactattac ccctttccct tttaaccgtt
     4321 catgcatggt tttttcaggt tggcgttgtg aatagatata acgtaaatcc gtatcaggca
     4381 agctatccca gtcgctgggc ttgactacct ttatagccga aaccttttta ctcttggagg
     4441 gcttttctag ttctgacact accgtttcgc cttccatcaa ccactgcaaa acaagaggca
     4501 gtaacttttg tcttgtcatc gttggattct ggctaaacgc ttttttaata gtgggaatta
     4561 acaggcttga atcgtaatcc tctttcagca tcagatataa atgacgctga tcttcggtat
     4621 ctaactcttg aagggctttt tcttctggtg tttgttcttg tatcgctgcg atatctagct
     4681 gaagttggat taaccgttta tcaatctcgg gaacgactag atcagaataa cgagcaatgc
     4741 gttctgggat acccgagcgt aatgcctcta gcatggggtg cacaggcttt aactcatctt
     4801 gatacacttg ccgcaataaa ccagcggtaa tacgctcatt gcctaaagct agcgcacgga
     4861 gctgagcgag tacaaaaagt tttactacaa tgtccatcac tccttggctt agctcatacc
     4921 acacatcacg gacctcatcc gataacagcg catctttgcg ttgtaaaagc tgtaattgcc
     4981 aaagattatc cgtaaaagcg atccactctt gattgggctt tccacgttgc gtttgttgta
     5041 taggatccca gaatatagct ccaaaccctg cccctctacg tgcagaccgc aaatcagcct
     5101 caaaaatctc tcgtgcttta ggggtaccaa tcaacatcac tggtacgcca ataatattca
     5161 ccatcgtcac aaaaaagttc agcatctctt gagatccacc cgaacgagag cggcttaaat
     5221 gctgaatttc atcaataacc aacaacccta aagcatgtgc attggctatt tgcgacatca
     5281 aagccaacat ggtttctata ccatgacgtt ttaagccata acgacgctca tagttcgagc
     5341 ccaaggctcg atccaacgct ctgaaaaaat tcaagcagat ttcttttagc gaaccattat
     5401 gcgagcagtc tattttcaaa tacaccacct gctctacatt gagttcacga tggtaaatca
     5461 cctgaggata cgtggctaga atacgatgaa gagaggtcgt cttcccacta ccagaacaac
     5521 caattaacaa taagctttgt gccgtagatc gtgcctcctc aaagcgaaat gtctccaact
     5581 ctcccgtttg aacacgctca taaccatttt gtaaatgctt ttgtaaatct cctgttttag
     5641 gatttctgcc tacgtagcca cctcgaatca tgaccgaaat acgctcactt agtagcaaat
     5701 gcgtacctaa tggctgaaaa tagtcatctg gaatacgaca aatggtatga gctctgataa
     5761 cacgggactt ttgcaagtca gaggaagtaa gctgtaaaga ggatttcagt gatgcagcac
     5821 tattcactga ctcttgtaat ggtggtaagg cctcgataaa aggattatca cgataagcct
     5881 ctacccccgt atcacgataa actgcttgaa tccgggtagc actcatgact catccttttc
     5941 tggtggatcc tgaaataatt caggcacgta tgtgggtagg ctgtaatctt cttgatcatc
     6001 cgcttccact gcgttgaaag gaataacttt agcctcatca cctgaagagc ttggcttcaa
     6061 atgctccgca cgttttttac gctccgaggt cacggcttct tttttattag ttttaatctg
     6121 cttaatgcgt gttgatttgg gctcagtagt actgggcgtt aacttattcg ctttctgaat
     6181 ggtttgctga ataaacgcct caagctccct gcgtttagtt aactcatcct gcttcgcatt
     6241 ggctttattg tgtttttctt gtgcttgtat atcccaaacc tcccaaaatg agagaccttt
     6301 aaactgccga ctacgttccg tcagattaca gcgccaaaat acacggctgc caacttgcgg
     6361 aaacaaataa atcgtatcaa ccagcactgg gtcataagcc gcttctaaat gttgaggtct
     6421 agctatatca gtgctccgct gcaaccaacc ctcacgcaga atctctgacc ccgagtaata
     6481 caaaccccac aaattaacgc caaatgaaga aatagagacc tttcggcgag gcagtaacgc
     6541 tactcgcaac tgctcttgct ccacagccct taaactacct gtacgatgct gcatacccca
     6601 ttgccatagc tggacaggaa tagacggtaa atctgtagga aaatcagcat ctcgatcgta
     6661 tttatccatc accagatggt tatttctgaa taagatcgta cgcaaaataa tttgtgtgaa
     6721 ctcaaatacc gacagagatg catctaacct atagtctgtt tcaccatggc ttttgatccg
     6781 actgccctct acaatgccag gtgcaaagga cttaaactcg gcttgtagtg ttctaaaagt
     6841 gctttccact atgcctttag catcgccacg tctaggtgga gcactttcca ctcgcacatt
     6901 aaaactagaa actaaggctt cgacctgatg actcattaat tcgccacggt ccgctagcaa
     6961 cacatctggc aaacctacac acggccagtc tgagctacta atctcaatat catgctgggc
     7021 acaaatggcc gttttgtcag agcaagcatt tacaaaagcc tgcatcgcca ccacataaga
     7081 cggattttca aagccgatat aaaagcccgt gatcatccga ctaaacacat caatcacaat
     7141 gtaaagcgtt ggtcttccta tgattttttg gcgatcatga tgatccacta aataaatatc
     7201 agcaatcgtg gcatcaatct cataacgact cccagggcct aacgcctgag aagtggctgt
     7261 actacttaag ggtcgtacgt cttttttata tacccctgct ttaactctag actttaagcg
     7321 ctgagcttta gggtattctc gatcataaaa ataacgaaac tgacgtagtg ttgggtaatc
     7381 ctcttgggga atgcgaggaa aatactgagc aaacaagtcc acaaatcgtc tataggcaac
     7441 ggtggtcttt gtaccttttt gatttaacag gtgcttttct atggtcaacc taaaaaggcg
     7501 ttcaatctcg ggcgttacct tggttccttc acccttacca tattctctgg ctcggccaat
     7561 ctttgctgtt cctgtcgctg aacgtctttc ccctggtgca ccgctgtttt tgtagtcagg
     7621 aattaatgca ttaggcgttt gaccacgctg ccagtaacgg cgtaacaact tataaactgt
     7681 agccttagta accttatgtt cttggaccac atgctcaacg agttcgcttc tgactttagg
     7741 gtcgaaacga tccttactat taataatagg aagaattttt cgataatcct cctcgcgctt
     7801 ctggaagctg acagaatcta cagacggctc ttctaaatca agatgtacat aagggtcatc
     7861 agttctaact aagcggcctt catctaaata ctgcattagc aacaactcag ccctagcttg
     7921 tggaactcct ttatcagcgc ttatttgcat ccagacaact tggccatcct ctatagccaa
     7981 aatgcgatac ggatcattat caaatagcac aacctcatta atttgccaca tagcgcaact
     8041 cctccatatt cactacttgg ctaatacaga gatctgcaca cttatttgcc ctgaaagact
     8101 tataaatatt gaacttaata aaaccatttg cggttaaggc tcgtatctca ctcaatgttt
     8161 tgcctaactc caaatcataa gcaatatcaa cctgcttaca gacattgata atgttttcat
     8221 ctcctttttc ttgcaggata tgggccaatg gggatagttg tgctaaaagc tccgcagaaa
     8281 cttcttctgt tttcactgaa taaagccatt caatattttc ttttactacg ggatttattt
     8341 ctttatcagt aaaaatgaac caaggaattt gcttttgctg ccaatagcga cgctctagtt
     8401 ctagtttttc taaggtacgc tcgtcttgta aggctgctgc aggtttgact tgaatagcaa
     8461 actgctcaaa aggaccatct ttgcagtcca ctaaaaaatc agtagacata acctgatcta
     8521 caccacgaat aacaggatgc ttaataccac tatctattgc aatctgcctg gtatcactag
     8581 gtaataaggg gaactgctcg cgtatatcta gcacgctgct ctcccactca agactgagaa
     8641 aaacagcaag ctctaagtca gatagcaaat gatggactcg tcccgtctta tgagaataaa
     8701 tacggtggga acgacctgaa gaaggaactt cttgtactgt tagccatgga atatagtctt
     8761 taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact tcagaaaatg
     8821 aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat aactggactg
     8881 atttcagttt acaactattc tgtctagttt aagacttggg taccgagctc gaattcgcta
     8941 gcccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagga
     9001 tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa
     9061 tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct
     9121 ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt ggttagcgag
     9181 aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct
     9241 tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg
     9301 ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat
     9361 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt
     9421 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc gggtgatatt
     9481 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
     9541 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg
     9601 tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcaccagcc gcatttcagc
     9661 gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc ggagctgctg
     9721 gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc
     9781 catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca
     9841 gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc gtcgcgtctg
     9901 tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga ggaccaacgt
     9961 atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac cgtcggtcgc
    10021 aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg caccggggcc
    10081 agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg
    10141 tcataattgg taacgaatca gacaattgac ggcttgacgg agtagcatag ggtttgcaga
    10201 atccctgctt cgtccatttg acaggcacat tatgcatcga tgataagctg tcaaacatga
    10261 gcagatcctc tacgccggac gcatcgtggc ctgtgggcgg acaataaagt cttaaactga
    10321 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
    10381 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
    10441 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggtcgacgcg
    10501 gccgtggcgc gcctcctagg tgctcgagga attcttcccg tgatggataa ataagggtaa
    10561 gattccgcgt aagtatcgcg ggggcgtaag tctggttata aggcgttatt gttgcaggca
    10621 gtttggtcac ggccagcgcg cagcaaccgg agcgtacaca agtgcgtgag gatggcgagc
    10681 actgcccggg gccaaaatgg caaataaaat agcctaataa tccagacgat tacccgtaat
    10741 atgtttaatc agggctatac ttagcacact tccactgtgt gtgactgtct ggtctgactg
    10801 agacaagttt tcaaggattc gtaacctcga ggtacaacca gtttgcaaga tgattaaaca
    10861 ataacttaag ttgaagttac cccattaaat ggatggctaa tatgactaaa aaaatttcat
    10921 tcattattaa cggccaggtt gaaatttttc ccgaaagtga tgatttagtg caatccatta
    10981 attttggtga taatagtgtt tacctgccaa tattgaataa ttctcatgta aaaaacatta
    11041 ttgattataa tgaaaataat aaattacggt tgcataatat tgtcaatttt ctctatacgg
    11101 tagggcaaag atggaaaaat gaagaatatt caaggcgcag gacatacatt cgtgatttaa
    11161 aaaaatatat gggatattca gaagcaatgg ccaagttaga ggccaactgg atatctatga
    11221 ttttatgttc taaaggtggc ctttatgatg ttgtagaaaa tgaacttggt tctcgccata
    11281 tcatggatga atggctacct caggatgaaa gttatattaa ggcttttccg aaaggtaagt
    11341 ctatacatct gttggcaggt aatgttccat tatctgggat catgtctata ttacgcgcaa
    11401 ttttaaccaa gaatcagtgt attataaaaa catcgtcaac cgatcccttt accgctaatg
    11461 cattagcgtt aagctttatc gatgtagacc ctaatcatcc gataacgcgc tctttgtctg
    11521 ttgtatattg gccacaccaa ggtgatacat cactcgcaaa agaaattatg caacatatgg
    11581 atgttattgt cgcttgggga ggggaagatg cgattaattg ggctgtagaa catgcaccac
    11641 cctatgctga cgtgattaaa tttggctcta aaaagagttt ttgcattatt gataatccag
    11701 ttgatttaac gtcagcagct accggtgcgg ctcatgatat ttgtttttac gatcagcgcg
    11761 cttgtttttc tgcccaaaac atatattaca tgggaaatca gtatgaggaa tttaagttag
    11821 cgttgataga aaaacttaat ctatatgcgc atatattacc aaacgccaaa aaagattttg
    11881 atgaaaaggc ggcctattct ttagtccaaa aagagagctt atttgctgga ttaaaagtag
    11941 aggtggatgt tcatcaacgt tggatgatta ttgagtcaaa tgcgggtgtg gaatttaatc
    12001 aaccacttgg cagatgtgtg tatcttcatc acgtcgataa tattgagcaa gtattgcctt
    12061 atgttcaaaa aaataagaca caaaccatat ctatttttcc ttgggaatcc gcatttaagt
    12121 atcgagatgc gttggcatta agaggtgcgg aaaggattgt agaagcagga atgaataata
    12181 tatttcgagt tggtggatct catgacggaa tgaggccgtt acaacgatta gtgacatata
    12241 tttctcatga gaggccatct cattatactg ctaaggatgt tgcggttgaa atagaacaga
    12301 ctcgattcct ggaagaagat aagttccttg tatttgtccc gtaataggta aaaagtatgg
    12361 aaaataaatc caaatataaa accatcgacc atgttctttg tgttgaagga aataaaaaaa
    12421 ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa gagaaagaat accattatta
    12481 ttgcgtcggg ttttgcccga aggatggatc attttgctgg tttagcggaa tatttatcgc
    12541 ggaatgggtt tcatgtgatt cgctatgatt cacttcacca cgttgggttg agttcaggga
    12601 caattgatga atttacaatg tctataggaa aacagagcct attagccgtg gttgattggt
    12661 taaatacacg aaaaataaat aaccgtggta ttttggcttc aagcttatct gcacggatag
    12721 tttatgcaag tctatctgaa attaatgttt catttttaat caccgcagtc ggtgttgtta
    12781 acttaagata tacgcttgaa agagctttag gatttgatta tctcagttta cccattaatg
    12841 aattgccgaa taatttggat tttgaaggcc ataaattggg tgctgaagtc tttgcgagag
    12901 attgccttga ttttggctgg gaagatttaa cttctacaat caatagcatg atgtatcttg
    12961 atataccgtt tattgctttt actgcaaata acgacaattg ggtaaagcaa gatgaagtta
    13021 tcacattgtt atcaaatatt cgtagtaatc gatgcaagat atattctttg ctagggagtt
    13081 cgcatgactt gggtgaaaac ttagtggtcc tgcgcaattt ttatcaatcg gttacgaagg
    13141 ctgctatcgc gatggataat gatcgtctgg atattgatgt tgatattatt gaaccatcat
    13201 tcgaacatct aactattgcg acagtcaatg aacgtcgaat gaaaattgag attgaaaatc
    13261 aagcgatttc gctgtcttaa aacctattgg gatagatatt accctataga tttcaagatg
    13321 gatcgcgacg gcaagggagc gaatcccggg agcatagcaa actatgtgac cggggtgagt
    13381 gagtgcagcc aacaaagaag caacttgaaa gataacgggt atagttaatt ctatcactca
    13441 aatataaggg ctctctatga aatttggaaa ctttttgctt acataccaac ccccccaatt
    13501 ttctcaaaca gaagtaatga aacgtttggt taaattaggt cgtatttctg aggagtgtgg
    13561 ttttgatact gtatggttac tggagcatca tttcacggag tttggtttgc ttggtaaccc
    13621 ttatgtcgct gctgcatatt tacttggtgc aaccaaaaaa ttgaatgtag ggactgcggc
    13681 tattgttctt cccaccgctc atccagtgcg ccaacttgaa gatgtgaatt tattggatca
    13741 aatgtcaaaa ggacgatttc ggtttggtat ttgtcggggg ctttacaata aagactttcg
    13801 cgtatttggc acggatatga ataacagtcg cgctttaacg gagtgctggt acgggttgat
    13861 aaaaaatggc atgacagagg gatatatgga agctgataat gaacatatca agttccataa
    13921 ggtaaaagta aacccgacag catatagtaa aggtggagcc cctgtttatg tggttgctga
    13981 atcagcctcg acaactgaat gggccgctca atttggttta ccgatgatat taagttggat
    14041 tataaatact aacgaaaaga aagcacagct tgagctttat aacgaggtgg ctcaagaata
    14101 tgggcacgat attcataata tcgaccattg cttatcatat ataacatctg taaattatga
    14161 ctcaaataaa gcgcaagaga tttgtcggga ttttctaggg cattggtatg attcttatgt
    14221 gaatgccacg accatttttg atgattcaga caaaacaaga ggttatgatt tcaataaagg
    14281 gcagtggcgt gactttgtat taaagggaca tagagatact aatcgccgca ttgattacag
    14341 ttacgaaatc aatcccgtgg gaaccccgca ggaatgcatt gacataattc aaaaagacat
    14401 tgatgccacg ggaatatcaa atatctgttg tgggtttgaa gcgaatggaa cagtagacga
    14461 aattattgct tccatgaagc tcttccagtc tgatgtcatg ccgtttctta aagaaaaaca
    14521 acgttcgcta ttatagtagc taaggaaaaa gaaatgaaat ttggattgtt cttccttaac
    14581 ttcatcaatt caacaactgt tcaagaacaa agtatagttc gcatgcagga aataacagag
    14641 tatgttgata agttgaattt tgaacagatt ttggtgtatg aaaatcattt ttcaggtaat
    14701 ggtgttgtcg gtgctcctct gactgtttct ggttttttgc tcggtttaac agaaaaaatt
    14761 aaaattggct cattgaatca catcattaca actcatcatc ctgtccgaat agcggaggag
    14821 gcttgcttat tggatcaatt aagcgaaggg agatttattt tagggtttag tgattgtgaa
    14881 aaaaaagatg aaatgcgtct ttttaatcgc cctgttgaat atcaacagca actatttgaa
    14941 gagtgttatg aaatcattaa cgatgcttta acaacaggct attgtaatcc cgataatgat
    15001 ttttatagtt tccctaaaat atcggtaaac ccccacgctt atacccaagg cgggcctcgg
    15061 agatatgtca cagcaaccag tcatcatatt gttgagtggg cggctaaaaa aggcattcct
    15121 ctcatcttta agtgggatga ctccaatgat gttagatatg aatatgctga aaggtataaa
    15181 gccgttgctg ataaatatgg tattgactta tcagcgatag atcatcagtt aatggtattg
    15241 gttaactata acgaagatag tcacaaagct aaacaagaga cgcgtgcatt tatccgtgat
    15301 tatgttcttg aaatgtatcc taatgaaaat ctcgaaaata aacttgaaga gataatcaca
    15361 gaaaacgctg tcggagatta tacggaatgt atagctgcgg ctaagctggc aattgaaaag
    15421 tgcggtgcaa aaagtgtatt gttatctttt gaaccaatga atgacttgat gcaccaaaaa
    15481 aacgtaatca atattgttaa tgataatatt aaaaagtacc acatgtagta aaagaatatg
    15541 gcagcaacgc tgccatattc tctaaattat ttggaggggt aaaacaggta tgacttcata
    15601 tgttgataaa caagagatca tagcaagctc agaaattgat gatttgattt tttccagcga
    15661 tccattagct tggtcttacg atgaacagga aaaaatcaga aacaaatttg ttcttgatgc
    15721 atttcgtaat cactataaac attgtcaaga ataccgtcac tactgtcagg tacacaaagt
    15781 agacgacaat attacggaaa ttgatgacat acctgtattc ccaacatcag tttttaagtt
    15841 tactcgctta ttaacttctc aggagaacga gattgaaagt tggtttacca gcagcggcac
    15901 gagtggttta aaaagtcagg tggcgcgtaa cagactaagt attgagagac tcttaggctc
    15961 tgtgagttat ggcatgaaat atgttggtag ttggtttgat catcaaatag agttggtcaa
    16021 cttagggcca gatagattta atgctcataa catttggttt aaatatgtta tgagcttggt
    16081 agaattatta tatcccacga catttaccgt aatggaagaa cgaatagatt ttgttaagac
    16141 attgaatagc cttgagcgaa taaaaaatca aggaaaagat atttgtctta tcggctcacc
    16201 atactttatt tatttgctct gccagtatat gaaagataaa aatatctcat tttatgggga
    16261 taaaaacctt tatatcataa cggggggcgg ctggaaaagt tatgaaaaag agtccctaaa
    16321 acgcgatgat ttcaatcatc ttttattcga cacgttcaac ctcaataata ttagtcaaat
    16381 ccgcgatata tttaatcaag ttgaactcaa cacttgtttc tttgaggatg aaatgcaacg
    16441 taaacgtgtt ccgccgtggg tatatgcgcg agcacttgat cctgaaacat tgaaacctgt
    16501 acctgatgga atgccgggtt tgatgagtta tatggatgcg tcatcaacga gttatccggc
    16561 atttattgtt accgatgatg tcgggataat gagcagagaa tatggtcaat atcctggtgt
    16621 acttgttgag attttacgtc gcgccaatac gagggcacag aaagggtgtg ctttaagctt
    16681 aaaccaagca tttaatagtt gatatactct atggatttta agatgcatcg cgatggcaag
    16741 ggagtgaatt cggatccgag ctcggtacca agcttgatgc atagcttgag tattctatag
    16801 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    16861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    16921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    16981 aacctgtcgt gccaggacta cggtcgtgcc aacttgcgtg ataactatcg tctggttgat
    17041 ggcgcatcga aagacggctg gttattcact gctgaacata ctcagagtgt cctgaagggc
    17101 tttaacaagt ttgttgttca gtacgctact gactcgatga cctcgcaggg taaagggctg
    17161 tcgcagggtt ctggcgttgc atttgataac gaaaaatttg cctacaatat caacaacaac
    17221 ggtcacatgc tgcgtatcct cgaccacggt gcgatctcca tgggcgacaa ctgggacatg
    17281 atgtacgtgg gtatgtacca ggatatcaac tgggataacg acaacggcac caagtggtgg
    17341 accgtcggta ttcgcccgat gtacaagtgg acgccaatca tgagcaccgt gatggaaatc
    17401 ggctacgaca acgtcgaatc ccagcgcacc ggcgacaaga acaatcagta caaaattacc
    17461 ctcgcacaac aatggcaggc tggcgacagc atctggtcac gcccggctat tcgtgtcttc
    17521 gcaacctacg ccaagtggga tgagaaatgg ggttacgact acaccggtaa cgctgataac
    17581 aacgcgaact tcggcaaagc cgttcctgct gatttcaacg gcggcagctt cggtcgtggc
    17641 gacagcgacg agtggacctt cggtgcccag atggaaatct ggtggtaagc ttgcggccgc
    17701 tattgaggga tctgattaat taaaacgtgg cttactagga tccgatatca tttaaatcta
    17761 gggataacag ggtaatacta gtgtcgacca accagataag tgaaatctag ttccaaacta
    17821 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
    17881 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
    17941 ttttgtccgc ccacaggctg aaagcgctat ttcttcaaaa cccccgtcga cggtatcgat
    18001 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc
    18061 gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
    18121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    18181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    18241 gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg
    18301 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgtactgcg atgagtggca
    18361 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
    18421 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
    18481 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta
    18541 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
    18601 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg
    18661 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
    18721 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc
    18781 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg
    18841 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
    18901 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
    18961 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
    19021 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
    19081 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
    19141 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
    19201 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
    19261 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
    19321 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
    19381 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
    19441 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
    19501 atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt
    19561 tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt
    19621 taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt
    19681 tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc
    19741 aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa
    19801 aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc
    19861 gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc
    19921 ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
    19981 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
    20041 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
    20101 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
    20161 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
    20221 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
    20281 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
    20341 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag
    20401 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga
    20461 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa
    20521 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga
    20581 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac
    20641 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt
    20701 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga
    20761 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga
    20821 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt
    20881 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca
    20941 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg
    21001 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg
    21061 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc
    21121 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc
    21181 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg
    21241 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag
//
