LOCUS       sequence-330442-       10606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of GFP ligand in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->GFPlig
FEATURES             Location/Qualifiers
     source          1..10606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    152..269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        335..1513
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          566..1504
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1461..1481
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1530..1539
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1599..1601,1602..1604,1605..2315)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2572..3160
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2625..2645)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3043..3054)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3163..3179)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3164..3180)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3270..3503
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3528..3546)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3529..3546)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3836..3855)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4069..4088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4188..4210
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4248..4266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4334..4438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4439..4507,4508..5299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4657..4676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5470..6058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5959..5978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6212..6229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6299..6319)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6304..6633
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6484..6619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7767..7832
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7962..7982
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8407..8541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8444..8463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8498..8517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8709..9342
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9389..9514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10011..10244
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10429..10473
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
       61 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
      121 ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      181 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
      241 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
      301 tttggatcga taagcttgat atcgaattcc gtgaggctcc ggtgcccgtc agtgggcaga
      361 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      421 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      481 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      541 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      601 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      661 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      721 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      781 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      841 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      901 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
      961 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     1021 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     1081 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     1141 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     1201 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     1261 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     1321 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1381 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1441 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1501 tcaggtgtcg tgagaattct cacgcggccg ccaccatgga gaccgacacc ctcttgctct
     1561 gggtcctgct gctgtgggtt ccgggtagta ccggagacat ggtttctaag ggtgaagaat
     1621 tgttcaccgg agttgtgccc atcctggttg agctggacgg agacgttaac ggacacaagt
     1681 tctcagtgag cggcgaagga gagggcgacg ctacgtacgg taaactgacg ttgaagttca
     1741 tctgtacaac aggaaagctg ccagtgccct ggcctacact cgtgaccacc ctcacctacg
     1801 gagtccagtg cttctcccgg taccccgacc acatgaaaca gcacgatttc ttcaagtccg
     1861 cgatgcctga gggctatgtt caggaacgca ccatattctt caaggacgac ggtaactata
     1921 aaaccagggc tgaagttaag ttcgaggggg acacgttggt aaatagaatt gagttgaaag
     1981 gaatcgattt caaagaagac ggcaatattt tgggccacaa gctggagtac aactacaaca
     2041 gccacaacgt gtatattatg gctgataagc agaagaacgg catcaaggtg aacttcaaaa
     2101 tccgacacaa tatcgaggat ggctctgtgc aactggccga ccattaccag caaaacacgc
     2161 ctatcggcga cggtccggtt ttgctgccag ataaccacta cctctccacg caatcagccc
     2221 tctccaagga tcctaacgaa aaaagagacc atatggttct gcttgaattc gtgacggctg
     2281 ctgggatcac cctgggaatg gatgagctgt ataagaacgc cgtgggccag gatacgcagg
     2341 aggtgatcgt cgtgccccac agcttgccct tcaaggtcgt ggttattagt gctatcctgg
     2401 cgttggtggt gctgactatt atctccctta tcatacttat catgctgtgg caaaaaaaac
     2461 cccgataatt aattaaggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc
     2521 gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct
     2581 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2641 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2701 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2761 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2821 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2881 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2941 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3001 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3061 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3121 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     3181 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     3241 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3301 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     3361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3481 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     3541 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     3601 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     3661 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     3721 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     3781 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     3841 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     3901 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     3961 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     4021 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     4081 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4141 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4201 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4261 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4321 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4381 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4441 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4501 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4561 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4621 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4681 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4741 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4801 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4861 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4921 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     4981 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5041 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5101 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5161 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5221 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5281 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5341 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5401 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5461 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5521 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5581 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5641 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5701 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5761 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5821 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5881 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5941 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6001 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6061 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     6301 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6361 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6421 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6601 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     6661 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     6721 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     6781 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     6841 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     6901 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     6961 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     7021 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     7081 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     7141 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     7201 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     7261 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     7321 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     7381 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     7441 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     7501 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     7561 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     7621 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     7681 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     7741 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     7801 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     7861 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     7921 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     7981 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     8041 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     8101 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     8161 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     8221 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     8281 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     8341 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     8401 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8521 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     8581 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     8641 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     8701 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     8761 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     8821 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     8881 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     8941 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     9001 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     9061 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     9121 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     9181 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9241 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9301 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     9361 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9421 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9481 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9541 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     9601 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     9661 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     9721 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     9781 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     9841 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9901 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9961 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
    10021 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
    10081 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
    10141 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
    10201 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    10261 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10321 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    10381 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10441 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10501 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10561 tttgctgtac tttctatagt gaatagagtt aggcagggat attcac
//
