LOCUS       sequence-330444-       10516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of mCherry and PNE 
            anchor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_EF1a-->GssmCherry-PNE
FEATURES             Location/Qualifiers
     source          1..10516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    233..358
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    855..1088
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1273..1317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1602..1719
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1785..2963
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2016..2954
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2911..2931
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2980..2989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3037..3741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3179..3197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3447..3466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3932..4520
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3985..4005)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4403..4414)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4523..4539)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4524..4540)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4630..4863
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4888..4906)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4889..4906)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5196..5215)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5429..5448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5548..5570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5608..5626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5694..5798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5799..5867,5868..6659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6017..6036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6830..7418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7319..7338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7572..7589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7659..7679)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7664..7993
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7844..7979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9127..9192
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9322..9342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9767..9901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9804..9823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9858..9877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10069..10516,1..186)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
       61 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      121 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      181 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      241 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      301 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      361 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      421 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      481 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      541 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      601 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      661 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
      721 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
      781 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
      841 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
      901 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
      961 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1021 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1081 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1141 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1201 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1261 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1321 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     1381 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     1441 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     1501 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     1561 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
     1621 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1681 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     1741 agagatccag tttggatcga taagcttgat atcgaattcc gtgaggctcc ggtgcccgtc
     1801 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     1861 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     1921 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     1981 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2041 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2101 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2161 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2221 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2281 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2341 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2401 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2461 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2521 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2581 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     2641 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     2701 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     2761 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     2821 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     2881 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     2941 ttcttccatt tcaggtgtcg tgagaattct cacgcggccg ccaccatggg cgttaaagtc
     3001 cttttcgccc tgatctgcat tgccgtcgca gaggccgtga gcaagggcga ggaggataac
     3061 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3121 cacgagttcg agatcgaggg tgagggagag ggtcgcccat acgagggcac ccagaccgcc
     3181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggt
     3721 ggaatggacg agctgtacaa gttggttgga gaagcggctg ccaaggaggc cgctgcaaag
     3781 gctaattacc atctcgaaaa tgaggtcgcc cggctgaaga agttgtgatt aattaaggcc
     3841 gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca
     3901 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3961 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4021 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4081 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4141 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4201 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4261 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4321 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4381 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4441 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     4501 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
     4561 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4621 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4681 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4741 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4801 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4861 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     4921 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     4981 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     5041 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     5101 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     5161 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     5221 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     5281 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     5341 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     5401 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5461 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5521 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5581 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5641 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5701 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5761 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5821 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5881 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5941 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6001 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6061 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6121 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6181 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6241 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6301 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6361 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6421 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6481 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6541 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6601 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6661 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6721 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6781 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6841 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6901 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6961 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7021 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7081 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7141 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7201 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7261 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7381 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7441 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7501 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7561 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7621 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     7681 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7741 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7801 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7861 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7921 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7981 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     8041 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     8101 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     8161 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     8221 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     8281 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     8341 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     8401 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     8461 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     8521 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     8581 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     8641 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     8701 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     8761 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     8821 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     8881 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     8941 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     9001 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     9061 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     9121 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     9181 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     9241 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     9301 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     9361 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     9421 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     9481 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     9541 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     9601 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     9661 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9721 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9901 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9961 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    10021 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
    10081 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
    10141 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
    10201 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
    10261 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
    10321 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    10381 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
    10441 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
    10501 cagctgcttt ttgcct
//
