LOCUS       sequence-330445-       11046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_TRE-->2xNLS_IFP_PGK_PuroR
FEATURES             Location/Qualifiers
     source          1..11046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        305..672
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    349..367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    385..403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    421..439
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    457..475
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    493..511
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    529..547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      713..722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             728..748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             752..772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             785..1747
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="ARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADG
                     HSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDW
                     PAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTV
                     RELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRL
                     TADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQ
                     LWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     promoter        1760..2259
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1795..1818)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2192..2211
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2269..2865
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2269..2288)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2765..2785
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2969..3557
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3022..3042)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3440..3451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3560..3576)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3561..3577)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3667..3900
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3925..3943)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3926..3943)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4233..4252)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4466..4485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4585..4607
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4645..4663)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4731..4835
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4836..4904,4905..5696)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5054..5073)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5867..6455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6356..6375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6609..6626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6696..6716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6701..7030
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6881..7016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8164..8229
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8359..8379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8804..8938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8841..8860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8895..8914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9106..9739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9786..9911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10408..10641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10826..10870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
       61 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
      121 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      181 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      241 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcccgt ggaattatca
      301 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
      361 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
      421 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
      481 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
      541 atagagaacg tataagcttt gcttatgtaa accagggcgc ctataaaaga gtgctgattt
      601 tttgagtaaa cttcaattcc acaacacttt tgtcttatac caactttccg taccacttcc
      661 taccctcgta aagaattccc gtatacaccg gtgcaattct cacgcggccg ccgccaccat
      721 ggtggatcca aaaaagaaga gaaaggtaga tccaaagaag aaaaggaagg ttggcagcgg
      781 ctccgctcgg gaccctcaac ctttcttccc tcctctgtat ctcggaggac cagagatcac
      841 tactgagaac tgcgagcggg aacctataca tataccaggt tctattcaac ctcatggagc
      901 actgctcaca gccgatggac attctggcga ggtgctgcaa gtttcactca acgcagccac
      961 tttcctgggc caggaaccaa ctgttctgcg gggacagacc ctggcagccc tgctgccaga
     1021 ccagtggcct gctctgcaga ctgccctgcc tccaggctgc caggacgccc tgcagtatcg
     1081 cgccaccctg gattggcccg ccgccggaca cctgagcctg accgtgcata gggtggccga
     1141 gctgctgatt ctggaattcg agcctactga ggcatgggac tctattggac cccacgctct
     1201 gagaaatgca atgttcgctc tggagagtgc tcctaatctc cgggcactgg ctgaagtcgc
     1261 aacccaaaca gtccgggaac tgtcaggttt cgaccgggtg atgctgtaca agtttgcacc
     1321 agacgcaaca ggagaggtta ttgccgaagc aaggcgcgag ggcatgcagg cttacctcgg
     1381 gcataggttt cccgcatcca ccacccctgc acaagctagg gccctctaca caagacacct
     1441 gctccggctg accgcagaca ccagggctgc agcagtgccc ctcgaccccg tgctgaatcc
     1501 ccagacaaat gctcctacac ctctgggcgg agctgtcctc agagctacat ccccaatgca
     1561 catgcagtac ctgaggaata tgggagtggg ctcctccctg agcgtcagcg tcgtggtcgg
     1621 cggccagctg tggggactga ttgtctgcca ccatcagaca ccctacgtgc tgccacccga
     1681 tctgcggacc accctggagt atctggggag gctcctgtcc ctgcaggtgc agaggaaaga
     1741 agcctgagga tccttgactg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     1801 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     1861 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     1921 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     1981 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     2041 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     2101 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     2161 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     2221 gccgcgctgt tctcctcttc ctcatctccg ggcctttcgt ccgccaccat gaccgagtac
     2281 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     2341 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     2401 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     2461 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     2521 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     2581 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     2641 ctggccaccg tcggcgtgtc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     2701 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     2761 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     2821 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccttaat taaggccgca
     2881 actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcaggc
     2941 atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3001 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3061 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3121 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3181 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3241 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3301 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3361 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3421 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3481 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3541 ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac
     3601 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3661 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3721 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3781 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3841 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3901 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     3961 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     4021 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     4081 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     4141 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     4201 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     4261 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     4321 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     4381 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     4441 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4501 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4561 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4621 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4681 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4741 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4801 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4861 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4921 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4981 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5041 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5101 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5161 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5221 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5281 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5341 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5401 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5461 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5521 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5581 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5641 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5701 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5761 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5821 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5881 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5941 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6001 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6061 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6121 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6181 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6241 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6301 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6361 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6421 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6481 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6541 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6601 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6661 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     6721 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6781 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6841 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6901 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6961 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7021 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     7081 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     7141 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     7201 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     7261 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     7321 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     7381 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     7441 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     7501 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     7561 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     7621 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     7681 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     7741 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     7801 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     7861 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     7921 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     7981 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     8041 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     8101 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     8161 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     8221 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     8281 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     8341 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     8401 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     8461 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     8521 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     8581 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     8641 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     8701 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8761 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8821 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8881 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     8941 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     9001 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9061 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     9121 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     9181 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     9241 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     9301 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     9361 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     9421 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     9481 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     9541 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     9781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     9841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
    10021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
    10081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
    10141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
    10201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
    10261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    10321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    10381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    10441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    10501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    10561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    10621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    10681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    10741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    10801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    10861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    10921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    10981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    11041 acccga
//
