LOCUS       sequence-330446-        8841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_AU1.Ollas.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..8841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1052..1093
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1118..1141
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1904..2492
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1957..1977)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2375..2386)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2567..2800
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2872..2993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2909..2928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2963..2982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3033..3168
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3188..3207)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3189..3207)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3217..3234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3217..3233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3226..3248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3375..3830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3462..3481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3672..3693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3856..3960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3961..4029,4030..4821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4179..4198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4992..5580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5481..5500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5734..5751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5868..5889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5904..5909,5910..5927,5928..5934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5942..5958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5947..5969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5966..5982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5966..5982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6001..6021
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6003..6021
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6049..6275
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6276..6456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6503..6628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7121..7354
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7539..7583
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7881..8129
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7881..7901
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8052..8071
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8148..8223
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8271..8388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8528..8841,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8759..8841,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcgacac
     1021 ctatcggtac attggaggag gcgggggagg gagcggcttc gccaacgagc tgggccccag
     1081 gctgatgggc aagtgcggag gaggcggggg agggaactgg tcccaccccc aattcgagaa
     1141 acccacaggc ctgtacacac acagcggtga gtgctgcaaa gcctgcaacc tgggcgaggg
     1201 tgtggcccag ccttgtggag ccaaccagac cgtgtgtgag ccctgcctgg acagcgtgac
     1261 gttctccgac gtggtgagcg cgaccgagcc gtgcaagccg tgcaccgagt gcgtggggct
     1321 ccagagcatg tcggcgccgt gcgtggaggc cgacgacgcc gtgtgccgct gcgcctacgg
     1381 ctactaccag gatgagacga ctgggcgctg cgaggcgtgc cgcgtgtgcg aggcgggctc
     1441 gggcctcgtg ttctcctgcc aggacaagca gaacaccgtg tgcgaggagt gccccgacgg
     1501 cacgtattcc gacgaggcca accacgtgga cccgtgcctg ccctgcaccg tgtgcgagga
     1561 caccgagcgc cagctccgcg agtgcacacg ctgggccgac gccgagtgcg aggagatccc
     1621 tggccgttgg attacacggt ccacaccccc agagggctcg gacagcacag cccccagcac
     1681 ccaggagcct gaggcacctc cagaacaaga cctcatagcc agcacggtgg caggtgtggt
     1741 gaccacagtg atgggcagct cccagcccgt ggtgacccga ggcaccaccg acaacctcat
     1801 ccctgtctat tgctccatcc tggctgctgt ggttgtgggc cttgtggcct acatagcctt
     1861 caagaggtgg aacaggggta tcctctagag cggccgcgtc gacaatcaac ctctggatta
     1921 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     1981 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2041 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2101 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2161 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2221 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2281 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     2341 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2401 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc gccctcagac
     2461 gagtcggatc tccctttggg ccgcctcccc gcctgggtac ctttaagacc aatgacttac
     2521 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     2581 cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     2641 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     2701 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     2761 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     2821 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     2881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3001 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3061 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3121 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3181 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3241 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3361 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     3841 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3901 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3961 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4021 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4081 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4141 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4201 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4261 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4321 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4381 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4441 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4501 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4561 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4621 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4681 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4741 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4801 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4861 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4921 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4981 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5041 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5101 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5161 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5221 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5281 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     5881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     5941 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6001 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6061 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6121 agaaaaagca ccgtgcatgg cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6181 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6241 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6481 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6541 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6601 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6661 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6721 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6781 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6841 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6901 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6961 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7021 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7081 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7141 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7201 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7261 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7321 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7381 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7441 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7501 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7561 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7621 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7681 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7741 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7801 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagtatc
     7861 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     7921 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     7981 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     8041 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     8101 tatatatctt gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt ttagagctag
     8161 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     8221 tgcttttttt aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     8281 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     8341 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     8401 agggacagca gagatccact ttggccgcgg ctcgaggcga taagctttgc aaagatggat
     8461 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     8521 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     8581 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     8641 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     8701 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     8761 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     8821 ttgaattact tccacctggc t
//
