LOCUS       sequence-330451-        3768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SARS-CoV-2 WT spike protein with an 18aa deletion on the 
            C-terminal tail. Used for SARS2 pseudotyped virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Rogers TF, Zhao F, Huang D, Beutler N, Burns A, He WT, Limbo O, 
            Smith C, Song G, Woehl J, Yang L, Abbott RK, Callaghan S, Garcia E, 
            Hurtado J, Parren M, Peng L, Ramirez S, Ricketts J, Ricciardi MJ, 
            Rawlings SA, Wu NC, Yuan M, Smith DM, Nemazee D, Teijaro JR, Voss 
            JE, Wilson IA, Andrabi R, Briney B, Landais E, Sok D, Jardine JG, 
            Burton DR
  TITLE     Isolation of potent SARS-CoV-2 neutralizing antibodies and 
            protection from disease in a small animal model.
  JOURNAL   Science. 2020 Aug 21;369(6506):956-963. doi: 
            10.1126/science.abc7520. Epub 2020 Jun 15.
  PUBMED    32540903
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc7520"; journalName: "Science"; date: 
            "2020-08-21- 21"; volume: "369"; issue: "6506"; pages: "956-963"
COMMENT     Sequence Label: pcDNA3.3_CoV2_D18
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1313..1330)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atgtttgtgt tcctggtgct gctgccactg gtgtccagcc agtgtgtgaa cctgaccacc
       61 aggacccaac ttcctcctgc ctacaccaac tccttcacca ggggagtcta ctaccctgac
      121 aaggtgttca ggtcctctgt gctgcacagc acccaggacc tgttcctgcc attcttcagc
      181 aatgtgacct ggttccatgc catccatgtg tctggcacca atggcaccaa gaggtttgac
      241 aaccctgtgc tgccattcaa tgatggagtc tactttgcca gcacagagaa gagcaacatc
      301 atcaggggct ggatttttgg caccaccctg gacagcaaga cccagtccct gctgattgtg
      361 aacaatgcca ccaatgtggt gattaaggtg tgtgagttcc agttctgtaa tgacccattc
      421 ctgggagtct actaccacaa gaacaacaag tcctggatgg agtctgagtt cagggtctac
      481 tcctctgcca acaactgtac ctttgaatat gtgagccaac cattcctgat ggacttggag
      541 ggcaagcagg gcaacttcaa gaacctgagg gagtttgtgt tcaagaacat tgatggctac
      601 ttcaagattt acagcaaaca cacaccaatc aacctggtga gggacctgcc acagggcttc
      661 tctgccttgg aaccactggt ggacctgcca attggcatca acatcaccag gttccagacc
      721 ctgctggctc tgcacaggtc ctacctgaca cctggagact cctcctctgg ctggacagca
      781 ggagcagcag cctactatgt gggctacctc caaccaagga ccttcctgct gaaatacaat
      841 gagaatggca ccatcacaga tgctgtggac tgtgccctgg acccactgtc tgagaccaag
      901 tgtaccctga aatccttcac agtggagaag ggcatctacc agaccagcaa cttcagggtc
      961 caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag
     1021 gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac
     1081 tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat
     1141 ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt
     1201 gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac
     1261 tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac
     1321 ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac
     1381 ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt
     1441 aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc
     1501 aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc
     1561 cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac
     1621 ttcaacttca atggactgac aggcacagga gtgctgacag agagcaacaa gaagttcctg
     1681 ccattccaac agtttggcag ggacattgct gacaccacag atgctgtgag ggacccacag
     1741 accttggaga ttctggacat cacaccatgt tcctttggag gagtgtctgt gattacacct
     1801 ggcaccaaca ccagcaacca ggtggctgtg ctctaccagg atgtgaactg tactgaggtg
     1861 cctgtggcta tccatgctga ccaacttaca ccaacctgga gggtctacag cacaggcagc
     1921 aatgtgttcc agaccagggc tggctgtctg attggagcag agcatgtgaa caactcctat
     1981 gagtgtgaca tcccaattgg agcaggcatc tgtgcctcct accagaccca gaccaacagc
     2041 ccaaggaggg caaggtctgt ggcaagccag agcatcattg cctacacaat gagtctggga
     2101 gcagagaact ctgtggctta cagcaacaac agcattgcca tcccaaccaa cttcaccatc
     2161 tctgtgacca cagagattct gcctgtgagt atgaccaaga cctctgtgga ctgtacaatg
     2221 tatatctgtg gagacagcac agagtgtagc aacctgctgc tccaatatgg ctccttctgt
     2281 acccaactta acagggctct gacaggcatt gctgtggaac aggacaagaa cacccaggag
     2341 gtgtttgccc aggtgaagca gatttacaag acacctccaa tcaaggactt tggaggcttc
     2401 aacttcagcc agattctgcc tgacccaagc aagccaagca agaggtcctt cattgaggac
     2461 ctgctgttca acaaggtgac cctggctgat gctggcttca tcaagcaata tggagactgt
     2521 ctgggagaca ttgctgccag ggacctgatt tgtgcccaga agttcaatgg actgacagtg
     2581 ctgcctccac tgctgacaga tgagatgatt gcccaataca cctctgccct gctggctggc
     2641 accatcacct ctggctggac ctttggagca ggagcagccc tccaaatccc atttgctatg
     2701 cagatggctt acaggttcaa tggcattgga gtgacccaga atgtgctcta tgagaaccag
     2761 aaactgattg ccaaccagtt caactctgcc attggcaaga ttcaggactc cctgtccagc
     2821 acagcctctg ccctgggcaa actccaagat gtggtgaacc agaatgccca ggctctgaac
     2881 accctggtga agcaactttc cagcaacttt ggagccatct cctctgtgct gaatgacatc
     2941 ctgagcagac tggacaaggt ggaggctgag gtccagattg acagactgat tacaggcaga
     3001 ctccaatccc tccaaaccta tgtgacccaa caacttatca gggctgctga gattagggca
     3061 tctgccaacc tggctgccac caagatgagt gagtgtgtgc tgggacaaag caagagggtg
     3121 gacttctgtg gcaagggcta ccacctgatg agttttccac agtctgcccc tcatggagtg
     3181 gtgttcctgc atgtgaccta tgtgcctgcc caggagaaga acttcaccac agcccctgcc
     3241 atctgccatg atggcaaggc tcactttcca agggagggag tgtttgtgag caatggcacc
     3301 cactggtttg tgacccagag gaacttctat gaaccacaga ttatcaccac agacaacacc
     3361 tttgtgtctg gcaactgtga tgtggtgatt ggcattgtga acaacacagt ctatgaccca
     3421 ctccaacctg aactggactc cttcaaggag gaactggaca aatacttcaa gaaccacacc
     3481 agccctgatg tggacctggg agacatctct ggcatcaatg cctctgtggt gaacatccag
     3541 aaggagattg acagactgaa tgaggtggct aagaacctga atgagtccct gattgacctc
     3601 caagaactgg gcaaatatga acaatacatc aagtggccat ggtacatctg gctgggcttc
     3661 attgctggac tgattgccat tgtgatggtg accataatgc tgtgttgtat gacctcctgt
     3721 tgttcctgtc tgaaaggctg ttgttcctgt ggctcctgtt gtaagtga
//
