LOCUS       sequence-330453-        3684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SARS-CoV-1 WT spike protein with a 28aa deletion on the 
            C-terminal tail. Used for SARS1 pseudotyped virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Rogers TF, Zhao F, Huang D, Beutler N, Burns A, He WT, Limbo O, 
            Smith C, Song G, Woehl J, Yang L, Abbott RK, Callaghan S, Garcia E, 
            Hurtado J, Parren M, Peng L, Ramirez S, Ricketts J, Ricciardi MJ, 
            Rawlings SA, Wu NC, Yuan M, Smith DM, Nemazee D, Teijaro JR, Voss 
            JE, Wilson IA, Andrabi R, Briney B, Landais E, Sok D, Jardine JG, 
            Burton DR
  TITLE     Isolation of potent SARS-CoV-2 neutralizing antibodies and 
            protection from disease in a small animal model.
  JOURNAL   Science. 2020 Aug 21;369(6506):956-963. doi: 
            10.1126/science.abc7520. Epub 2020 Jun 15.
  PUBMED    32540903
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc7520"; journalName: "Science"; date: 
            "2020-08-21- 21"; volume: "369"; issue: "6506"; pages: "956-963"
COMMENT     Sequence Label: pcDNA3.3_CoV1_D28
FEATURES             Location/Qualifiers
     source          1..3684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttcatct tcctgctgtt cctgaccctg acctctggct ctgacctgga caggtgtacc
       61 acctttgatg atgtccaggc tccaaactac acccaacaca cctccagtat gaggggagtc
      121 tactaccctg atgagatttt caggtctgac accctctacc tgacccagga cctgttcctg
      181 ccattctaca gcaatgtgac aggcttccac accatcaacc acacctttga caaccctgtg
      241 attccattca aggatggcat ctactttgct gccacagaga agagcaatgt ggtgaggggc
      301 tgggtgtttg gcagcacaat gaacaacaag agccagtctg tgattatcat caacaacagc
      361 accaatgtgg tgattagggc ttgtaacttt gaactgtgtg acaacccatt ctttgctgtg
      421 agcaagccta tgggcaccca gacccacaca atgatttttg acaatgcctt caactgtacc
      481 tttgaataca tctctgatgc cttctccctg gatgtgtctg agaagtctgg caacttcaaa
      541 cacctgaggg agtttgtgtt caagaacaag gatggcttcc tctatgtcta caagggctac
      601 caaccaattg atgtggtgag ggacctgcca tctggcttca acaccctgaa accaatcttc
      661 aaactgccac tgggcatcaa catcaccaac ttcagggcta tcctgacagc cttcagccct
      721 gcccaggaca cctggggcac ctctgctgct gcctactttg tgggctacct gaaaccaacc
      781 acctttatgc tgaaatatga tgagaatggc accatcacag atgctgtgga ctgtagccag
      841 aacccactgg ctgaactgaa atgttctgtg aagtcctttg agattgacaa gggcatctac
      901 cagaccagca acttcagggt ggtgccatct ggagatgtgg tgaggtttcc aaacatcacc
      961 aacctgtgtc catttggaga ggtgttcaat gccaccaagt ttccatctgt ctatgcctgg
     1021 gagaggaaga agattagcaa ctgtgtggct gactactctg tgctctacaa cagcaccttc
     1081 ttcagcacct tcaagtgtta tggagtgtct gccaccaaac tgaatgacct gtgtttcagc
     1141 aatgtctatg ctgactcctt tgtggtgaag ggagatgatg tgagacagat tgcccctgga
     1201 caaacaggag tgattgctga ctacaactac aaactgcctg atgactttat gggctgtgtg
     1261 ctggcttgga acaccaggaa cattgatgcc accagcacag gcaactacaa ctacaaatac
     1321 agatacctga gacatggcaa actgagacca tttgagaggg acatcagcaa tgtgccattc
     1381 agccctgatg gcaagccatg tactcctcct gccctgaact gttactggcc actgaatgac
     1441 tatggcttct acaccaccac aggcattggc taccaaccat acagggtggt ggtgctgtcc
     1501 tttgaactgc tgaatgcccc tgccacagtg tgtggaccaa aactgagcac agacctgatt
     1561 aagaaccagt gtgtgaactt caacttcaat ggactgacag gcacaggagt gctgacacca
     1621 tccagcaaga ggttccaacc attccaacag tttggcaggg atgtgtctga cttcacagac
     1681 tctgtgaggg acccaaagac ctctgagatt ctggacatca gcccatgttc ctttggagga
     1741 gtgtctgtga ttacacctgg caccaatgcc tcctctgagg tggctgtgct ctaccaggat
     1801 gtgaactgta ctgatgtgag cacagccatc catgctgacc aacttacacc tgcctggagg
     1861 atttacagca caggcaacaa tgtgttccag acccaggctg gctgtctgat tggagcagag
     1921 catgtggaca cctcctatga gtgtgacatc ccaattggag caggcatctg tgcctcctac
     1981 cacacagtgt ccctgctgag gagcaccagc cagaagagca ttgtggctta cacaatgagt
     2041 ctgggagcag actccagcat tgcctacagc aacaacacca ttgccatccc aaccaacttc
     2101 agcatcagca tcaccacaga ggtgatgcct gtgagtatgg ctaagacctc tgtggactgt
     2161 aatatgtata tctgtggaga cagcacagag tgtgccaacc tgctgctcca atatggctcc
     2221 ttctgtaccc aacttaacag ggctctgtct ggcattgctg ctgaacagga caggaacacc
     2281 agggaggtgt ttgcccaggt gaagcagatg tataagacac caaccctgaa atactttgga
     2341 ggcttcaact tcagccagat tctgcctgac ccactgaaac caaccaagag gtccttcatt
     2401 gaggacctgc tgttcaacaa ggtgaccctg gctgatgctg gctttatgaa gcaatatgga
     2461 gagtgtctgg gagacatcaa tgccagggac ctgatttgtg cccagaagtt caatggactg
     2521 acagtgctgc ctccactgct gacagatgat atgattgctg cctacacagc agccctggtg
     2581 tctggcacag ccacagcagg ctggaccttt ggagcaggag cagccctcca aatcccattt
     2641 gctatgcaga tggcttacag gttcaatggc attggagtga cccagaatgt gctctatgag
     2701 aaccagaagc agattgccaa ccagttcaac aaggctatca gccagattca ggagtccctg
     2761 accaccacca gcacagccct gggcaaactc caagatgtgg tgaaccagaa tgcccaggct
     2821 ctgaacaccc tggtgaagca actttccagc aactttggag ccatctcctc tgtgctgaat
     2881 gacatcctga gcagactgga caaggtggag gctgaggtcc agattgacag actgattaca
     2941 ggcagactcc aatccctcca aacctatgtg acccaacaac ttatcagggc tgctgagatt
     3001 agggcatctg ccaacctggc tgccaccaag atgagtgagt gtgtgctggg acaaagcaag
     3061 agggtggact tctgtggcaa gggctaccac ctgatgagtt ttccacaggc tgcccctcat
     3121 ggagtggtgt tcctgcatgt gacctatgtg ccaagccagg agaggaactt caccacagcc
     3181 cctgccatct gccatgaggg caaggcttac tttccaaggg agggagtgtt tgtgttcaat
     3241 ggcacctcct ggttcatcac ccagaggaac ttcttcagcc cacagattat caccacagac
     3301 aacacctttg tgtctggcaa ctgtgatgtg gtgattggca tcatcaacaa cacagtctat
     3361 gacccactcc aacctgaact ggactccttc aaggaggaac tggacaaata cttcaagaac
     3421 cacaccagcc ctgatgtgga cctgggagac atctctggca tcaatgcctc tgtggtgaac
     3481 atccagaagg agattgacag actgaatgag gtggctaaga acctgaatga gtccctgatt
     3541 gacctccaag aactgggcaa atatgaacaa tacatcaagt ggccatggta tgtgtggctg
     3601 ggcttcattg ctggactgat tgccattgtg atggtgacca tcctgctgtg ttgtatgacc
     3661 tcctgttgtt cctgtctgaa ataa
//
