LOCUS       sequence-330479-        8874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_S.C.V5_NGFR
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1016..1060
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1079..1114
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             1133..1174
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1937..2525
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1990..2010)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2408..2419)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2600..2833
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2905..3026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2942..2961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2996..3015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3066..3201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3221..3240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3222..3240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3250..3267)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3250..3266)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3259..3281)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3408..3863
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3495..3514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3705..3726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3889..3993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3994..4062,4063..4854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4212..4231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5025..5613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5514..5533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5767..5784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5901..5922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5937..5942,5943..5960,5961..5967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5975..5991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5980..6002
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5999..6015
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5999..6015
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6034..6054
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6036..6054
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6082..6308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6309..6489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6536..6661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7154..7387
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7572..7616
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7914..8162
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7914..7934
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8085..8104
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8181..8256
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8304..8421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8561..8874,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8792..8874,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcaagga
     1021 gaccgccgcc gccaagttcg agaggcagca catggacagc ggaggaggcg ggggagggga
     1081 ggaccaggtg gaccccaggc tgatcgacgg caagggagga ggcgggggag ggggcaaacc
     1141 aattccaaac ccactgctgg gactggattc cacacccaca ggcctgtaca cacacagcgg
     1201 tgagtgctgc aaagcctgca acctgggcga gggtgtggcc cagccttgtg gagccaacca
     1261 gaccgtgtgt gagccctgcc tggacagcgt gacgttctcc gacgtggtga gcgcgaccga
     1321 gccgtgcaag ccgtgcaccg agtgcgtggg gctccagagc atgtcggcgc cgtgcgtgga
     1381 ggccgacgac gccgtgtgcc gctgcgccta cggctactac caggatgaga cgactgggcg
     1441 ctgcgaggcg tgccgcgtgt gcgaggcggg ctcgggcctc gtgttctcct gccaggacaa
     1501 gcagaacacc gtgtgcgagg agtgccccga cggcacgtat tccgacgagg ccaaccacgt
     1561 ggacccgtgc ctgccctgca ccgtgtgcga ggacaccgag cgccagctcc gcgagtgcac
     1621 acgctgggcc gacgccgagt gcgaggagat ccctggccgt tggattacac ggtccacacc
     1681 cccagagggc tcggacagca cagcccccag cacccaggag cctgaggcac ctccagaaca
     1741 agacctcata gccagcacgg tggcaggtgt ggtgaccaca gtgatgggca gctcccagcc
     1801 cgtggtgacc cgaggcacca ccgacaacct catccctgtc tattgctcca tcctggctgc
     1861 tgtggttgtg ggccttgtgg cctacatagc cttcaagagg tggaacaggg gtatcctcta
     1921 gagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1981 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2041 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2101 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2161 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2221 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2281 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     2341 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2401 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2461 gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2521 cccgcctggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2581 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     2641 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2701 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2761 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2821 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     2881 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     2941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3001 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     3061 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3121 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3181 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     3241 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3301 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3361 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3841 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     3901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4201 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5821 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5881 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5941 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6001 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     6061 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     6121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg
     6181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     6241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     6301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     6541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     6601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     6661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     6721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     6781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     6841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     6901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     6961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     7021 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     7081 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     7141 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     7201 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7261 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7321 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7381 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7441 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7501 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7561 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     7621 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     7681 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     7741 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     7801 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     7861 gatctcgacg gtatcgatca cgagactagt atcgagcggc cgcccccttc accgagggcc
     7921 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     7981 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     8041 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     8101 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     8161 ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc
     8221 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc gacctcgaga
     8281 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     8341 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8401 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     8461 cggctcgagg cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     8521 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     8581 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     8641 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     8701 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     8761 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     8821 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggct
//
