LOCUS       sequence-330482-        8859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_S.C.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..8859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1016..1060
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1079..1114
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             1136..1159
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1922..2510
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1975..1995)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2393..2404)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2585..2818
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2890..3011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2927..2946)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2981..3000
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3051..3186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3206..3225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3207..3225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3235..3252)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3235..3251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3244..3266)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3393..3848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3480..3499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3690..3711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3874..3978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3979..4047,4048..4839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4197..4216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5010..5598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5499..5518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5752..5769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5886..5907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5922..5927,5928..5945,5946..5952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5960..5976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5965..5987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5984..6000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5984..6000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6019..6039
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6021..6039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6067..6293
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6294..6474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6521..6646
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7139..7372
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7557..7601
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7899..8147
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7899..7919
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8070..8089
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8166..8241
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8289..8406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8546..8859,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8777..8859,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcaagga
     1021 gaccgccgcc gccaagttcg agaggcagca catggacagc ggaggaggcg ggggagggga
     1081 ggaccaggtg gaccccaggc tgatcgacgg caagggagga ggcgggggag ggaactggtc
     1141 ccacccccaa ttcgagaaac ccacaggcct gtacacacac agcggtgagt gctgcaaagc
     1201 ctgcaacctg ggcgagggtg tggcccagcc ttgtggagcc aaccagaccg tgtgtgagcc
     1261 ctgcctggac agcgtgacgt tctccgacgt ggtgagcgcg accgagccgt gcaagccgtg
     1321 caccgagtgc gtggggctcc agagcatgtc ggcgccgtgc gtggaggccg acgacgccgt
     1381 gtgccgctgc gcctacggct actaccagga tgagacgact gggcgctgcg aggcgtgccg
     1441 cgtgtgcgag gcgggctcgg gcctcgtgtt ctcctgccag gacaagcaga acaccgtgtg
     1501 cgaggagtgc cccgacggca cgtattccga cgaggccaac cacgtggacc cgtgcctgcc
     1561 ctgcaccgtg tgcgaggaca ccgagcgcca gctccgcgag tgcacacgct gggccgacgc
     1621 cgagtgcgag gagatccctg gccgttggat tacacggtcc acacccccag agggctcgga
     1681 cagcacagcc cccagcaccc aggagcctga ggcacctcca gaacaagacc tcatagccag
     1741 cacggtggca ggtgtggtga ccacagtgat gggcagctcc cagcccgtgg tgacccgagg
     1801 caccaccgac aacctcatcc ctgtctattg ctccatcctg gctgctgtgg ttgtgggcct
     1861 tgtggcctac atagccttca agaggtggaa caggggtatc ctctagagcg gccgcgtcga
     1921 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1981 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2041 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2101 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2161 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2221 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2281 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     2341 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2401 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcatct
     2461 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgggtacct
     2521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2581 ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
     2641 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2701 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2761 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2821 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     2881 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3001 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     3061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3121 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3181 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     3241 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3301 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3361 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     3421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3841 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4201 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5821 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6001 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     6061 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     6121 aacatgcctt acaaggagag aaaaagcacc gtgcatggcg attggtggaa gtaaggtggt
     6181 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     6241 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     6301 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6361 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6421 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     6481 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     6541 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     6601 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     6661 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     6721 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     6781 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     6841 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     6901 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     6961 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     7021 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     7081 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     7141 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     7201 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     7261 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     7321 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     7381 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7441 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     7501 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     7561 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     7621 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     7681 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     7741 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     7801 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     7861 gatcacgaga ctagtatcga gcggccgccc ccttcaccga gggcctattt cccatgattc
     7921 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     7981 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     8041 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     8101 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa
     8161 gacctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     8221 aaagtggcac cgagtcggtg ctttttttaa ttctcgacct cgagacaaat ggcagtattc
     8281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8401 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgaggcgata
     8461 agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     8521 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     8581 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     8641 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     8701 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     8761 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     8821 ttatggccct tgcgtgcctt gaattacttc cacctggct
//
