LOCUS       sequence-330484-       23437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gentamycin resistant plasmid for Tn7-mediated chromosomal 
            integration of Photorhabdus luminescens luxCDABE operon with Pfrr 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23437)
  AUTHORS   Matsumoto A, Schluter T, Melkonian K, Takeda A, Nakagami H, Mine A
  TITLE     A versatile Tn7 transposon-based bioluminescence tagging tool for 
            quantitative and spatial detection of bacteria in plants.
  JOURNAL   Plant Commun. 2021 Jul 20;3(1):100227. doi: 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10.
  PUBMED    35059625
REFERENCE   2  (bases 1 to 23437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2021-07-20"; volume: "3(1):100227. doi"; pages: " 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10"
COMMENT     Sequence Label: pBJ5
FEATURES             Location/Qualifiers
     source          1..23437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  26..191
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1037..1058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1073..1078,1079..1096,1097..1103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1111..1127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1135..1151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1169..1187
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1169..1186
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(7835..8713)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        8740..9024
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     8947..8966
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(15715..15732)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      15885..15971
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      16063..16090
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(16171..16191)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16171..16189)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16210..16226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16234..16250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16258..16264,16265..16282,16283..16288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16303..16324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16594..16599,16600..16616,16617..16622)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             16811..17344
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      18626..19397
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             19398..20060
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLVHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      20272..20803
                     /label=oriV
                     /note="incP origin of replication"
     CDS             21532..22677
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDLVHCKR"
     primer_bind     23274..23296
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     23289..23305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     23315..23334
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23315..23333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctggaagaa atagcgcttt cagcctgtgg gcggacaaaa tagttgggaa ctgggagggg
       61 tggaaatgga gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt
      121 gtagcgtcgt aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca
      181 cttatctggt tggtcgacac tagtattacc ctgttatccc tagatttaaa tgatatcgga
      241 tcctagtaag ccacgtttta attaatcaga tccctcaata gcggccgcaa gcttaccacc
      301 agatttccat ctgggcaccg aaggtccact cgtcgctgtc gccacgaccg aagctgccgc
      361 cgttgaaatc agcaggaacg gctttgccga agttcgcgtt gttatcagcg ttaccggtgt
      421 agtcgtaacc ccatttctca tcccacttgg cgtaggttgc gaagacacga atagccgggc
      481 gtgaccagat gctgtcgcca gcctgccatt gttgtgcgag ggtaattttg tactgattgt
      541 tcttgtcgcc ggtgcgctgg gattcgacgt tgtcgtagcc gatttccatc acggtgctca
      601 tgattggcgt ccacttgtac atcgggcgaa taccgacggt ccaccacttg gtgccgttgt
      661 cgttatccca gttgatatcc tggtacatac ccacgtacat catgtcccag ttgtcgccca
      721 tggagatcgc accgtggtcg aggatacgca gcatgtgacc gttgttgttg atattgtagg
      781 caaatttttc gttatcaaat gcaacgccag aaccctgcga cagcccttta ccctgcgagg
      841 tcatcgagtc agtagcgtac tgaacaacaa acttgttaaa gcccttcagg acactctgag
      901 tatgttcagc agtgaataac cagccgtctt tcgatgcgcc atcaaccaga cgatagttat
      961 cacgcaagtt ggcacgaccg tagtcctggc acgacaggtt tcccgactgg aaagcgggca
     1021 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     1081 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     1141 acagctatga ccatgattac gccaagctat ttaggtgaca ctatagaata ctcaagctat
     1201 gcatcaagct tggtaccgag ctcggatccg aattcactcc cttgccatcg cgatgcatct
     1261 taaaatccat agagtatatc aactattaaa tgcttggttt aagcttaaag cacacccttt
     1321 ctgtgccctc gtattggcgc gacgtaaaat ctcaacaagt acaccaggat attgaccata
     1381 ttctctgctc attatcccga catcatcggt aacaataaat gccggataac tcgttgatga
     1441 cgcatccata taactcatca aacccggcat tccatcaggt acaggtttca atgtttcagg
     1501 atcaagtgct cgcgcatata cccacggcgg aacacgttta cgttgcattt catcctcaaa
     1561 gaaacaagtg ttgagttcaa cttgattaaa tatatcgcgg atttgactaa tattattgag
     1621 gttgaacgtg tcgaataaaa gatgattgaa atcatcgcgt tttagggact ctttttcata
     1681 acttttccag ccgccccccg ttatgatata aaggttttta tccccataaa atgagatatt
     1741 tttatctttc atatactggc agagcaaata aataaagtat ggtgagccga taagacaaat
     1801 atcttttcct tgatttttta ttcgctcaag gctattcaat gtcttaacaa aatctattcg
     1861 ttcttccatt acggtaaatg tcgtgggata taataattct accaagctca taacatattt
     1921 aaaccaaatg ttatgagcat taaatctatc tggccctaag ttgaccaact ctatttgatg
     1981 atcaaaccaa ctaccaacat atttcatgcc ataactcaca gagcctaaga gtctctcaat
     2041 acttagtctg ttacgcgcca cctgactttt taaaccactc gtgccgctgc tggtaaacca
     2101 actttcaatc tcgttctcct gagaagttaa taagcgagta aacttaaaaa ctgatgttgg
     2161 gaatacaggt atgtcatcaa tttccgtaat attgtcgtct actttgtgta cctgacagta
     2221 gtgacggtat tcttgacaat gtttatagtg attacgaaat gcatcaagaa caaatttgtt
     2281 tctgattttt tcctgttcat cgtaagacca agctaatgga tcgctggaaa aaatcaaatc
     2341 atcaatttct gagcttgcta tgatctcttg tttatcaaca tatgaagtca tacctgtttt
     2401 acccctccaa ataatttaga gaatatggca gcgttgctgc catattcttt tactacatgt
     2461 ggtacttttt aatattatca ttaacaatat tgattacgtt tttttggtgc atcaagtcat
     2521 tcattggttc aaaagataac aatacacttt ttgcaccgca cttttcaatt gccagcttag
     2581 ccgcagctat acattccgta taatctccga cagcgttttc tgtgattatc tcttcaagtt
     2641 tattttcgag attttcatta ggatacattt caagaacata atcacggata aatgcacgcg
     2701 tctcttgttt agctttgtga ctatcttcgt tatagttaac caataccatt aactgatgat
     2761 ctatcgctga taagtcaata ccatatttat cagcaacggc tttatacctt tcagcatatt
     2821 catatctaac atcattggag tcatcccact taaagatgag aggaatgcct tttttagccg
     2881 cccactcaac aatatgatga ctggttgctg tgacatatct ccgaggcccg ccttgggtat
     2941 aagcgtgggg gtttaccgat attttaggga aactataaaa atcattatcg ggattacaat
     3001 agcctgttgt taaagcatcg ttaatgattt cataacactc ttcaaatagt tgctgttgat
     3061 attcaacagg gcgattaaaa agacgcattt catctttttt ttcacaatca ctaaacccta
     3121 aaataaatct cccttcgctt aattgatcca ataagcaagc ctcctccgct attcggacag
     3181 gatgatgagt tgtaatgatg tgattcaatg agccaatttt aattttttct gttaaaccga
     3241 gcaaaaaacc agaaacagtc agaggagcac cgacaacacc attacctgaa aaatgatttt
     3301 catacaccaa aatctgttca aaattcaact tatcaacata ctctgttatt tcctgcatgc
     3361 gaactatact ttgttcttga acagttgttg aattgatgaa gttaaggaag aacaatccaa
     3421 atttcatttc tttttcctta gctactataa tagcgaacgt tgtttttctt taagaaacgg
     3481 catgacatca gactggaaga gcttcatgga agcaataatt tcgtctactg ttccattcgc
     3541 ttcaaaccca caacagatat ttgatattcc cgtggcatca atgtcttttt gaattatgtc
     3601 aatgcattcc tgcggggttc ccacgggatt gatttcgtaa ctgtaatcaa tgcggcgatt
     3661 agtatctcta tgtcccttta atacaaagtc acgccactgc cctttattga aatcataacc
     3721 tcttgttttg tctgaatcat caaaaatggt cgtggcattc acataagaat cataccaatg
     3781 ccctagaaaa tcccgacaaa tctcttgcgc tttatttgag tcataattta cagatgttat
     3841 atatgataag caatggtcga tattatgaat atcgtgccca tattcttgag ccacctcgtt
     3901 ataaagctca agctgtgctt tcttttcgtt agtatttata atccaactta atatcatcgg
     3961 taaaccaaat tgagcggccc attcagttgt cgaggctgat tcagcaacca cataaacagg
     4021 ggctccacct ttactatatg ctgtcgggtt tacttttacc ttatggaact tgatatgttc
     4081 attatcagct tccatatatc cctctgtcat gccatttttt atcaacccgt accagcactc
     4141 cgttaaagcg cgactgttat tcatatccgt gccaaatacg cgaaagtctt tattgtaaag
     4201 cccccgacaa ataccaaacc gaaatcgtcc ttttgacatt tgatccaata aattcacatc
     4261 ttcaagttgg cgcactggat gagcggtggg aagaacaata gccgcagtcc ctacattcaa
     4321 ttttttggtt gcaccaagta aatatgcagc agcgacataa gggttaccaa gcaaaccaaa
     4381 ctccgtgaaa tgatgctcca gtaaccatac agtatcaaaa ccacactcct cagaaatacg
     4441 acctaattta accaaacgtt tcattacttc tgtttgagaa aattgggggg gttggtatgt
     4501 aagcaaaaag tttccaaatt tcatagagag cccttatatt tgagtgatag aattaactat
     4561 acccgttatc tttcaagttg cttctttgtt ggctgcactc actcaccccg gtcacatagt
     4621 ttgctatgct cccgggattc gctcccttgc cgtcgcgatc catcttgaaa tctatagggt
     4681 aatatctatc ccaataggtt ttaagacagc gaaatcgctt gattttcaat ctcaattttc
     4741 attcgacgtt cattgactgt cgcaatagtt agatgttcga atgatggttc aataatatca
     4801 acatcaatat ccagacgatc attatccatc gcgatagcag ccttcgtaac cgattgataa
     4861 aaattgcgca ggaccactaa gttttcaccc aagtcatgcg aactccctag caaagaatat
     4921 atcttgcatc gattactacg aatatttgat aacaatgtga taacttcatc ttgctttacc
     4981 caattgtcgt tatttgcagt aaaagcaata aacggtatat caagatacat catgctattg
     5041 attgtagaag ttaaatcttc ccagccaaaa tcaaggcaat ctctcgcaaa gacttcagca
     5101 cccaatttat ggccttcaaa atccaaatta ttcggcaatt cattaatggg taaactgaga
     5161 taatcaaatc ctaaagctct ttcaagcgta tatcttaagt taacaacacc gactgcggtg
     5221 attaaaaatg aaacattaat ttcagataga cttgcataaa ctatccgtgc agataagctt
     5281 gaagccaaaa taccacggtt atttattttt cgtgtattta accaatcaac cacggctaat
     5341 aggctctgtt ttcctataga cattgtaaat tcatcaattg tccctgaact caacccaacg
     5401 tggtgaagtg aatcatagcg aatcacatga aacccattcc gcgataaata ttccgctaaa
     5461 ccagcaaaat gatccatcct tcgggcaaaa cccgacgcaa taataatggt attctttctc
     5521 tttgggctgt tttcttctgg cagcgtttcc caaacatgaa tttttttatt tccttcaaca
     5581 caaagaacat ggtcgatggt tttatatttg gatttatttt ccatactttt tacctattac
     5641 gggacaaata caaggaactt atcttcttcc aggaatcgag tctgttctat ttcaaccgca
     5701 acatccttag cagtataatg agatggcctc tcatgagaaa tatatgtcac taatcgttgt
     5761 aacggcctca ttccgtcatg agatccacca actcgaaata tattattcat tcctgcttct
     5821 acaatccttt ccgcacctct taatgccaac gcatctcgat acttaaatgc ggattcccaa
     5881 ggaaaaatag atatggtttg tgtcttattt ttttgaacat aaggcaatac ttgctcaata
     5941 ttatcgacgt gatgaagata cacacatctg ccaagtggtt gattaaattc cacacccgca
     6001 tttgactcaa taatcatcca acgttgatga acatccacct ctacttttaa tccagcaaat
     6061 aagctctctt tttggactaa agaataggcc gccttttcat caaaatcttt tttggcgttt
     6121 ggtaatatat gcgcatatag attaagtttt tctatcaacg ctaacttaaa ttcctcatac
     6181 tgatttccca tgtaatatat gttttgggca gaaaaacaag cgcgctgatc gtaaaaacaa
     6241 atatcatgag ccgcaccggt agctgctgac gttaaatcaa ctggattatc aataatgcaa
     6301 aaactctttt tagagccaaa tttaatcacg tcagcatagg gtggtgcatg ttctacagcc
     6361 caattaatcg catcttcccc tccccaagcg acaataacat ccatatgttg cataatttct
     6421 tttgcgagtg atgtatcacc ttggtgtggc caatatacaa cagacaaaga gcgcgttatc
     6481 ggatgattag ggtctacatc gataaagctt aacgctaatg cattagcggt aaagggatcg
     6541 gttgacgatg tttttataat acactgattc ttggttaaaa ttgcgcgtaa tatagacatg
     6601 atcccagata atggaacatt acctgccaac agatgtatag acttaccttt cggaaaagcc
     6661 ttaatataac tttcatcctg aggtagccat tcatccatga tatggcgaga accaagttca
     6721 ttttctacaa catcataaag gccaccttta gaacataaaa tcatagatat ccagttggcc
     6781 tctaacttgg ccattgcttc tgaatatccc atatattttt ttaaatcacg aatgtatgtc
     6841 ctgcgccttg aatattcttc atttttccat ctttgcccta ccgtatagag aaaattgaca
     6901 atattatgca accgtaattt attattttca ttataatcaa taatgttttt tacatgagaa
     6961 ttattcaata ttggcaggta aacactatta tcaccaaaat taatggattg cactaaatca
     7021 tcactttcgg gaaaaatttc aacctggccg ttaataatga atgaaatttt tttagtcata
     7081 ttagccatcc atttaatggg gtaacttcaa cttaagttat tgtttaatca tcttgcaaac
     7141 tggttgtacc tcgaggttac gaatccttga aaacttgtct cagtcagacc agacagtcac
     7201 acacagtgga agtgtgctaa gtatagccct gattaaacat attacgggta atcgtctgga
     7261 ttattaggct attttatttg ccattttggc cccgggcagt gctcgccatc ctcacgcact
     7321 tgtgtacgct ccggttgctg cgcgctggcc gtgaccaaac tgcctgcaac aataacgcct
     7381 tataaccaga cttacgcccc cgcgatactt acgcggaatc ttacccttat ttatccatca
     7441 cgggaagaat tcctcgagca cctaggaggc gcgccacggc cgcgtcgacc ccacgcccct
     7501 ctttaatacg acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa
     7561 aagtccagta tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc
     7621 tagtttaaga ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt
     7681 ccgcccacag gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca
     7741 tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact
     7801 ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat
     7861 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     7921 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     7981 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     8041 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     8101 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     8161 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     8221 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     8281 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     8341 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     8401 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     8461 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     8521 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     8581 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     8641 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     8701 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     8761 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     8821 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     8881 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     8941 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     9001 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcgaattc gagctcggta
     9061 cccaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
     9121 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
     9181 gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat tccatggcta
     9241 acagtacaag aagttccttc ttcaggtcgt tcccaccgta tttattctca taagacggga
     9301 cgagtccatc atttgctatc tgacttagag cttgctgttt ttctcagtct tgagtgggag
     9361 agcagcgtgc tagatatacg cgagcagttc cccttattac ctagtgatac caggcagatt
     9421 gcaatagata gtggtattaa gcatcctgtt attcgtggtg tagatcaggt tatgtctact
     9481 gattttttag tggactgcaa agatggtcct tttgagcagt ttgctattca agtcaaacct
     9541 gcagcagcct tacaagacga gcgtacctta gaaaaactag aactagagcg tcgctattgg
     9601 cagcaaaagc aaattccttg gttcattttt actgataaag aaataaatcc cgtagtaaaa
     9661 gaaaatattg aatggcttta ttcagtgaaa acagaagaag tttctgcgga gcttttagca
     9721 caactatccc cattggccca tatcctgcaa gaaaaaggag atgaaaacat tatcaatgtc
     9781 tgtaagcagg ttgatattgc ttatgatttg gagttaggca aaacattgag tgagatacga
     9841 gccttaaccg caaatggttt tattaagttc aatatttata agtctttcag ggcaaataag
     9901 tgtgcagatc tctgtattag ccaagtagtg aatatggagg agttgcgcta tgtggcaaat
     9961 taatgaggtt gtgctatttg ataatgatcc gtatcgcatt ttggctatag aggatggcca
    10021 agttgtctgg atgcaaataa gcgctgataa aggagttcca caagctaggg ctgagttgtt
    10081 gctaatgcag tatttagatg aaggccgctt agttagaact gatgaccctt atgtacatct
    10141 tgatttagaa gagccgtctg tagattctgt cagcttccag aagcgcgagg aggattatcg
    10201 aaaaattctt cctattatta atagtaagga tcgtttcgac cctaaagtca gaagcgaact
    10261 cgttgagcat gtggtccaag aacataaggt tactaaggct acagtttata agttgttacg
    10321 ccgttactgg cagcgtggtc aaacgcctaa tgcattaatt cctgactaca aaaacagcgg
    10381 tgcaccaggg gaaagacgtt cagcgacagg aacagcaaag attggccgag ccagagaata
    10441 tggtaagggt gaaggaacca aggtaacgcc cgagattgaa cgccttttta ggttgaccat
    10501 agaaaagcac ctgttaaatc aaaaaggtac aaagaccacc gttgcctata gacgatttgt
    10561 ggacttgttt gctcagtatt ttcctcgcat tccccaagag gattacccaa cactacgtca
    10621 gtttcgttat ttttatgatc gagaataccc taaagctcag cgcttaaagt ctagagttaa
    10681 agcaggggta tataaaaaag acgtacgacc cttaagtagt acagccactt ctcaggcgtt
    10741 aggccctggg agtcgttatg agattgatgc cacgattgct gatatttatt tagtggatca
    10801 tcatgatcgc caaaaaatca taggaagacc aacgctttac attgtgattg atgtgtttag
    10861 tcggatgatc acgggctttt atatcggctt tgaaaatccg tcttatgtgg tggcgatgca
    10921 ggcttttgta aatgcttgct ctgacaaaac ggccatttgt gcccagcatg atattgagat
    10981 tagtagctca gactggccgt gtgtaggttt gccagatgtg ttgctagcgg accgtggcga
    11041 attaatgagt catcaggtcg aagccttagt ttctagtttt aatgtgcgag tggaaagtgc
    11101 tccacctaga cgtggcgatg ctaaaggcat agtggaaagc acttttagaa cactacaagc
    11161 cgagtttaag tcctttgcac ctggcattgt agagggcagt cggatcaaaa gccatggtga
    11221 aacagactat aggttagatg catctctgtc ggtatttgag ttcacacaaa ttattttgcg
    11281 tacgatctta ttcagaaata accatctggt gatggataaa tacgatcgag atgctgattt
    11341 tcctacagat ttaccgtcta ttcctgtcca gctatggcaa tggggtatgc agcatcgtac
    11401 aggtagttta agggctgtgg agcaagagca gttgcgagta gcgttactgc ctcgccgaaa
    11461 ggtctctatt tcttcatttg gcgttaattt gtggggtttg tattactcgg ggtcagagat
    11521 tctgcgtgag ggttggttgc agcggagcac tgatatagct agacctcaac atttagaagc
    11581 ggcttatgac ccagtgctgg ttgatacgat ttatttgttt ccgcaagttg gcagccgtgt
    11641 attttggcgc tgtaatctga cggaacgtag tcggcagttt aaaggtctct cattttggga
    11701 ggtttgggat atacaagcac aagaaaaaca caataaagcc aatgcgaagc aggatgagtt
    11761 aactaaacgc agggagcttg aggcgtttat tcagcaaacc attcagaaag cgaataagtt
    11821 aacgcccagt actactgagc ccaaatcaac acgcattaag cagattaaaa ctaataaaaa
    11881 agaagccgtg acctcggagc gtaaaaaacg tgcggagcat ttgaagccaa gctcttcagg
    11941 tgatgaggct aaagttattc ctttcaacgc agtggaagcg gatgatcaag aagattacag
    12001 cctacccaca tacgtgcctg aattatttca ggatccacca gaaaaggatg agtcatgagt
    12061 gctacccgga ttcaagcagt ttatcgtgat acgggggtag aggcttatcg tgataatcct
    12121 tttatcgagg ccttaccacc attacaagag tcagtgaata gtgctgcatc actgaaatcc
    12181 tctttacagc ttacttcctc tgacttgcaa aagtcccgtg ttatcagagc tcataccatt
    12241 tgtcgtattc cagatgacta ttttcagcca ttaggtacgc atttgctact aagtgagcgt
    12301 atttcggtca tgattcgagg tggctacgta ggcagaaatc ctaaaacagg agatttacaa
    12361 aagcatttac aaaatggtta tgagcgtgtt caaacgggag agttggagac atttcgcttt
    12421 gaggaggcac gatctacggc acaaagctta ttgttaattg gttgttctgg tagtgggaag
    12481 acgacctctc ttcatcgtat tctagccacg tatcctcagg tgatttacca tcgtgaactc
    12541 aatgtagagc aggtggtgta tttgaaaata gactgctcgc ataatggttc gctaaaagaa
    12601 atctgcttga attttttcag agcgttggat cgagccttgg gctcgaacta tgagcgtcgt
    12661 tatggcttaa aacgtcatgg tatagaaacc atgttggctt tgatgtcgca aatagccaat
    12721 gcacatgctt tagggttgtt ggttattgat gaaattcagc atttaagccg ctctcgttcg
    12781 ggtggatctc aagagatgct gaactttttt gtgacgatgg tgaatattat tggcgtacca
    12841 gtgatgttga ttggtacccc taaagcacga gagatttttg aggctgattt gcggtctgca
    12901 cgtagagggg cagggtttgg agctatattc tgggatccta tacaacaaac gcaacgtgga
    12961 aagcccaatc aagagtggat cgcttttacg gataatcttt ggcaattaca gcttttacaa
    13021 cgcaaagatg cgctgttatc ggatgaggtc cgtgatgtgt ggtatgagct aagccaagga
    13081 gtgatggaca ttgtagtaaa actttttgta ctcgctcagc tccgtgcgct agctttaggc
    13141 aatgagcgta ttaccgctgg tttattgcgg caagtgtatc aagatgagtt aaagcctgtg
    13201 caccccatgc tagaggcatt acgctcgggt atcccagaac gcattgctcg ttattctgat
    13261 ctagtcgttc ccgagattga taaacggtta atccaacttc agctagatat cgcagcgata
    13321 caagaacaaa caccagaaga aaaagccctt caagagttag ataccgaaga tcagcgtcat
    13381 ttatatctga tgctgaaaga ggattacgat tcaagcctgt taattcccac tattaaaaaa
    13441 gcgtttagcc agaatccaac gatgacaaga caaaagttac tgcctcttgt tttgcagtgg
    13501 ttgatggaag gcgaaacggt agtgtcagaa ctagaaaagc cctccaagag taaaaaggtt
    13561 tcggctataa aggtagtcaa gcccagcgac tgggatagct tgcctgatac ggatttacgt
    13621 tatatctatt cacaacgcca acctgaaaaa accatgcatg aacggttaaa agggaaaggg
    13681 gtaatagtgg atatggcgag cttatttaaa caagcaggtt agccatgaga aactttcctg
    13741 ttccgtactc gaatgagctg atttatagca ctattgcacg ggcaggcgtt tatcaaggga
    13801 ttgttagtcc taagcagctg ttggatgagg tgtatggcaa ccgcaaggtg gtcgctacct
    13861 taggtctgcc ctcgcattta ggtgtgatag caagacatct acatcaaaca ggacgttacg
    13921 ctgttcagca gcttatttat gagcatacct tattcccttt atatgctccg tttgtaggca
    13981 aggagcgccg agacgaagct attcggttaa tggagtacca agcgcaaggt gcggtgcatt
    14041 taatgctagg agtcgctgct tctagagtta agagcgataa ccgctttaga tactgccctg
    14101 attgcgttgc tcttcagcta aataggtatg gggaagcctt ttggcaacga gattggtatt
    14161 tgcccgcttt gccatattgt ccaaaacacg gtgctttagt cttctttgat agagctgtag
    14221 atgatcaccg acatcaattt tgggctttgg gtcatactga gctgctttca gactacccca
    14281 aagactccct atctcaatta acagcactag ctgcttatat agcccctctg ttagatgctc
    14341 cacgagcgca agagctttcc ccaagccttg agcagtggac gctgttttat cagcgcttag
    14401 cgcaggatct agggctaacc aaaagcaagc acattcgtca tgacttggtg gcggagagag
    14461 tgaggcagac ttttagtgat gaggcactag agaaactgga tttaaagttg gcagagaaca
    14521 aggacacgtg ttggctgaaa agtatattcc gtaagcatag aaaagccttt agttatttac
    14581 agcatagtat tgtgtggcaa gccttattgc caaaactaac ggttatagaa gcgctacagc
    14641 aggcaagtgc tcttactgag cactctataa cgacaagacc tgttagccag tctgtgcaac
    14701 ctaactctga agatttatct gttaagcata aagactggca gcaactagtg cataaatacc
    14761 aaggaattaa ggcggcaaga cagtctttag agggtggggt gctatacgct tggctttacc
    14821 gacatgacag ggattggcta gttcactgga atcaacagca tcaacaagag cgtctggcac
    14881 ccgcccctag agttgattgg aaccaaagag atcgaattgc tgtacgacaa ctattaagaa
    14941 tcataaagcg tctagatagt agccttgatc acccaagagc gacatcgagc tggctgttaa
    15001 agcaaactcc taacggaacc tctcttgcaa aaaatctaca gaaactgcct ttggtagcgc
    15061 tttgcttaaa gcgttactca gagagtgtgg aagattatca aattagacgg attagccaag
    15121 cttttattaa gcttaaacag gaagatgttg agcttaggcg ctggcgatta ttaagaagtg
    15181 caacgttatc taaagagcgg ataactgagg aagcacaaag attcttggaa atggtttatg
    15241 gggaagagtg agtggttagg ctagctacat ttaatgacaa tgtgcaggtt gtacatattg
    15301 gtcatttatt ccgtaactcg ggtcataagg agtggcgtat ttttgtttgg tttaatccaa
    15361 tgcaagaacg gaaatggact cgatttactc atttgccttt attaagtcga gctaaggtgg
    15421 ttaacagtac aacaaagcaa ataaataagg cggatcgtgt gattgagttt gaagcatcgg
    15481 atcttcaacg agccaaaata atcgattttc ctaatctctc gtcctttgct tccgtacgca
    15541 acaaggatgg agcgcagagt tcatttattt acgaagctga aacaccatat agcaagactc
    15601 gttatcacat cccacagtta gagctagctc ggtcattatt tttaggggat cctctagagt
    15661 cgacctgcag gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
    15721 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
    15781 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
    15841 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
    15901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
    15961 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
    16021 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
    16081 atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg
    16141 tatccgcggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
    16201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    16261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    16321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    16381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgca tgcataaaaa
    16441 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
    16501 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggacgc
    16561 acaccgtgga aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa
    16621 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
    16681 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct
    16741 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
    16801 agcagcaacg atgttacgca gcagcaacga tgttacgcag cagggcagtc gccctaaaac
    16861 aaagttaggt ggctcaagta tgggcatcat tcgcacatgt aggctcggcc ctgaccaagt
    16921 caaatccatg cgggctgctc ttgatctttt cggtcgtgag ttcggagacg tagccaccta
    16981 ctcccaacat cagccggact ccgattacct cgggaacttg ctccgtagta agacattcat
    17041 cgcgcttgct gccttcgacc aagaagcggt tgttggcgct ctcgcggctt acgttctgcc
    17101 caagtttgag cagccgcgta gtgagatcta tatctatgat ctcgcagtct ccggagagca
    17161 ccggaggcag ggcattgcca ccgcgctcat caatctcctc aagcatgagg ccaacgcgct
    17221 tggtgcttat gtgatctacg tgcaagcaga ttacggtgac gatcccgcag tggctctcta
    17281 tacaaagttg ggcatacggg aagaagtgat gcactttgat atcgacccaa gtaccgccac
    17341 ctaacaattc gttcaagccg agatcggctt cccggccgcg gagttgttcg gtaaattgtc
    17401 acaacgccgc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg
    17461 ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt
    17521 gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac
    17581 gggcttcagg cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg
    17641 catctcacgc gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt
    17701 tgtcagccgg tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc
    17761 ggccaatccc tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg
    17821 gcccactggt ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt
    17881 gctgccctcg atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg
    17941 gtcgcgggtc atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg
    18001 cgtcagcggc accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc
    18061 cgtcacgatg cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa
    18121 cgccgcctgc tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta
    18181 ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc
    18241 catcgcttca tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc
    18301 agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat
    18361 tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg
    18421 gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc
    18481 ttgagtatac tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg
    18541 ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc
    18601 gtggatatgt ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg
    18661 gacaaccctg ctggacaagc tgatggacag gctgcgcctg cccacgagct tgaccacagg
    18721 gattgcccac cggctaccca gccttcgacc acatacccac cggctccaac tgcgcggcct
    18781 gcggccttgc cccatcaatt tttttaattt tctctgggga aaagcctccg gcctgcggcc
    18841 tgcgcgcttc gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga
    18901 ccgcgcagcg gcttggcctt gacgcgcctg gaacgaccca agcctatgcg agtgggggca
    18961 gtcgaagggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
    19021 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
    19081 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
    19141 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
    19201 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
    19261 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
    19321 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccct
    19381 accgcatgga gataagcatg gccacgcagt ccagagaaat cggcattcaa gccaagaaca
    19441 agcccggtca ctgggtgcaa acggaacgca aagcgcatga ggcgtgggcc gggcttattg
    19501 cgaggaaacc cacggcggca atgctgctgc atcacctcgt ggcgcagatg ggccaccaga
    19561 acgccgtggt ggtcagccag aagacacttt ccaagctcat cggacgttct ttgcggacgg
    19621 tccaatacgc agtcaaggac ttggtggccg agcgctggat ctccgtcgtg aagctcaacg
    19681 gccccggcac cgtgtcggcc tacgtggtca atgaccgcgt ggcgtggggc cagccccgcg
    19741 accagttgcg cctgtcggtg ttcagtgccg ccgtggtggt tgatcacgac gaccaggacg
    19801 aatcgctgtt ggtgcatggc gacctgcgcc gcatcccgac cctgtatccg ggcgagcagc
    19861 aactaccgac cggccccggc gaggagccgc ccagccagcc cggcattccg ggcatggaac
    19921 cagacctgcc agccttgacc gaaacggagg aatgggaacg gcgcgggcag cagcgcctgc
    19981 cgatgcccga tgagccgtgt tttctggacg atggcgagcc gttggagccg ccgacacggg
    20041 tcacgctgcc gcgccggtag cacttgggtt gcgcagcaac ccgtaagtgc gctgttccag
    20101 actatcggct gtagccgcct cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
    20161 ggtgcatgga gccgggccac ctcgacctga atggaagccg tatccacctt aacttaatga
    20221 tttttaccaa aatcattagg ggattcatca gagccgtgtg cgagacaccg cggccgccgg
    20281 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
    20341 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
    20401 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
    20461 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
    20521 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
    20581 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
    20641 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
    20701 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
    20761 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
    20821 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
    20881 agtccttgcc attgccggga tcggggcagt aacgggatgg gcgatcagcc cgagcgcgac
    20941 gcccggaagc attgacgtgc cgcaggtgct ggcatcgaca ttcagcgacc aggtgccggg
    21001 cagtgagggc ggcggcctgg gtggcggcct gcccttcact tcggccgtcg gggcattcac
    21061 ggacttcatg gcggggccgg caatttttac cttgggcatt cttggcatag tggtcgcggg
    21121 tgccgtgctc gtgttcgggg gtgaattaat tccccggatc cgtcagcttc acgctgccgc
    21181 aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
    21241 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
    21301 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
    21361 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
    21421 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
    21481 tcaagatcga cggatccggg gaattccggg gcaatcccgc aaggagggtg aatgaatcgg
    21541 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
    21601 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
    21661 ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
    21721 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg
    21781 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
    21841 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg
    21901 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
    21961 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
    22021 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    22081 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    22141 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
    22201 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
    22261 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
    22321 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
    22381 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
    22441 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
    22501 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
    22561 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
    22621 ggcagcggcc tggtggaaca cgcctgggtc aatgacctgg tgcattgcaa acgctagggc
    22681 cttgtggggt cagttccggc tgggggttca gcacagctgg gcggcacctc gctaacggat
    22741 tcaccgtttt tatcaggctc tgggaggcag aataaatgat catatcgtca attattacct
    22801 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacacg gtcacactgc
    22861 ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
    22921 gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
    22981 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
    23041 ttacgccccg ccctgccact catcgcagta cggcctattg gttaaaaaat gagctgattt
    23101 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
    23161 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    23221 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    23281 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
    23341 ggtcgagggg gtgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag
    23401 ccagtgacgc ctcccgtggg gaaaaaatca tggcaat
//
