LOCUS       sequence-330495-        8885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria gene circuit on medium copy backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Zhang R, Goetz H, Melendez-Alvarez J, Li J, Ding T, Wang X, Tian XJ
  TITLE     Winner-takes-all resource competition redirects cascading cell fate 
            transitions.
  JOURNAL   Nat Commun. 2021 Feb 8;12(1):853. doi: 10.1038/s41467-021-21125-3.
  PUBMED    33558556
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-8"; volume: "12(1):853. doi"; pages: " 
            10.1038/s41467-021-21125-3"
COMMENT     Sequence Label: pSB3K3-IC25
FEATURES             Location/Qualifiers
     source          1..8885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             159..170
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             756..788
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      836..907
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      923..950
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1103..1114
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1121..1834
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1121..1834
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1151..1179)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1774..1794
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1841..1873
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      1896..1967
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1983..2010
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2163..2174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2181..3059
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      3081..3152
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3168..3195
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3273..3284
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4065..4136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4152..4179
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4257..4268
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4275..4949
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      5005..5076
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5092..5119
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5197..5208
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5215..6093
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      6115..6186
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6202..6229
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6236..6256
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(6396..7211)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6396..7211)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7119..7138
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7203..7222
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7806..8350)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7885..7904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8818..8836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8840..8883)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agacattgat tatttgcacg gcgtcacact ttgctatgcc
       61 atagcaagat agtccataag attagcggat cctacctgac gctttttatc gcaactctct
      121 actgtttctc cattccctat cagtgataga tactagagaa agaggagaaa tactagatga
      181 ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc
      241 taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata
      301 accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata
      361 ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa
      421 gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat
      481 taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta
      541 caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg
      601 taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc
      661 gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc
      721 ctattaatga acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt
      781 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat
      841 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      901 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      961 agagacattg attatttgca cggcgtcaca ctttgctatg ccatagcaag atagtccata
     1021 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccataccgt
     1081 ttttttgggc tagctactag agaaagagga gaaatactag atgcgtaaag gagaagaact
     1141 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1201 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1261 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1321 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1381 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1441 gacacgtgct gaagtcaagt ttgaaggtgg tacccttgtt aatagaatcg agttaaaagg
     1501 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1561 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     1621 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     1681 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     1741 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     1801 tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta
     1861 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1921 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1981 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca
     2041 cggcgtcaca ctttgctatg ccatagcaag atagtccata agattagcgg atcctacctg
     2101 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag
     2161 agaaagagga gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact
     2221 cgtttaacgc ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt
     2281 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc
     2341 agggggtggt gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt
     2401 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc
     2461 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat
     2521 ttgccaatac gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt
     2581 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa
     2641 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac
     2701 cgatggataa tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca
     2761 attttgatat cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc
     2821 ttttccgcca gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc
     2881 aggcgaagct gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg
     2941 gttttgacga tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga
     3001 gcgagttccg tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat
     3061 aaactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     3121 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     3181 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacagggt tacgcaagaa
     3241 aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag atgaaaaaca
     3301 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     3361 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     3421 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta
     3481 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     3541 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     3601 aaaaatctcc aaatgtaatt aaagaagcga aaacagcagg tcttatcact gggtttagtt
     3661 tccctattca tacggctaac aatggcttcg gaatacttag ttttgcacat tcagaaaaag
     3721 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     3781 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     3841 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     3901 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     3961 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     4021 attgcccata ctttaaaaat taataaacta ctagagccag gcatcaaata aaacgaaagg
     4081 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     4141 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta
     4201 ggatcgtaca gggttacgca agaaaatggt ttgttatagt cgaataaata ctagagaaag
     4261 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
     4321 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
     4381 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
     4441 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
     4501 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
     4561 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
     4621 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
     4681 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
     4741 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
     4801 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
     4861 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
     4921 gtgctgaagg tcgtcactcc accggtgctg ctgcaaacga cgaaaactac gcagcagcag
     4981 tttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     5041 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     5101 tcgggtgggc ctttctgcgt ttatatacta gagacctgta ggatcgtaca gggttacgca
     5161 agaaaatggt ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatggct
     5221 gaagcgcaaa atgatcccct gctgccggga tactcgttta acgcccatct ggtggcgggt
     5281 ttaacgccga ttgaggccaa tggttatctc gattttttta tcgaccgacc gctgggaatg
     5341 aaaggttata ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga
     5401 gaatttgtct gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac
     5461 ggtcgtcatc cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc
     5521 tactggcatg aatggcttaa ctggccgtca atatttgcca atacgggttt ctttcgcccg
     5581 gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa
     5641 ggggaagggc gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg
     5701 cgcatggaag cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct
     5761 tgtcagtaca tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag
     5821 catgtttgcc tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc
     5881 gtcttaagct ggcgcgagga ccaacgcatc agccaggcga agctgctttt gagcactacc
     5941 cggatgccta tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg
     6001 cgagtattta aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa
     6061 aaagtgaatg atgtagccgt caagttgtca taataaacta ctagagccag gcatcaaata
     6121 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6181 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     6241 gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6301 gcgagcggta tcagctcact caaaggcggt aatctcgagt cccgtcaagt cagcgtaatg
     6361 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     6421 tgaaactgca atttattcat atcaggatta tcgataccat atttttgaaa aagccgtttc
     6481 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     6541 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     6601 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     6661 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     6721 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     6781 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     6841 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     6901 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     6961 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     7021 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     7081 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     7141 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga
     7201 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     7261 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     7321 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     7381 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     7441 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg
     7501 tatgagtcag caacaccttc ttcacgaggc agacctcagc gctagcggag tgtatactgg
     7561 cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa
     7621 ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac
     7681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
     7741 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
     7801 ccgtttttcc ataggctccg ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     7861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     7921 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     7981 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     8041 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     8161 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     8221 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcaa
     8281 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     8341 acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg aaaactcacg
     8401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8761 tccatggtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     8821 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     8881 ttctg
//
