LOCUS       sequence-330496-       11950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histidine tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 11950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_His6_GluA2
FEATURES             Location/Qualifiers
     source          1..11950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2580..3027
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3072..3092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3162..3183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3423..3442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3770..3791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3843..3899
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3981..3998
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6640..7228
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6693..6713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7111..7122)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7231..7247)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7232..7248)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7753..7933
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7959..7976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7965..8189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8235..8663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8322..8341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8532..8553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8672..8692)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8677..9006
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8857..8992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8919..8938
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9054..9101
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9120..9494
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9624..9745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9661..9680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9715..9734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9794..9810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9794..9810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9807..9829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9818..9834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9842..9848,9849..9866,9867..9872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9887..9908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10025..10042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10196..10784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10276..10295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10955..11746,11747..11815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11578..11597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11816..11920)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta atcgcatgct agagagggcc ctgcgtatga
     2581 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     2641 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     2701 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     2761 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     2821 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     2881 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     2941 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     3001 ggagtcgtgt cgtgcctgag agcgcagtcg gatccgccac catggctcca aaaaagaaga
     3061 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatgg
     3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3301 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     3841 gagctactaa cttcagcctg ctgaagcaag caggagatgt tgaagaaaac cccgggcctg
     3901 ctagcatgca aaagattatg catatttctg tcctcctttc tcctgtttta tggggactga
     3961 tttttggtgt ctcttctatg catcatcacc atcaccacaa cagcatacag ataggaggcc
     4021 tatttccaag gggcgctgat caagaataca gtgcatttcg ggtagggatg gttcagtttt
     4081 ccacttcgga gttcagactg acaccccata tcgacaattt ggaggtagcc aacagtttcg
     4141 cagtcaccaa tgctttctgc tcccagtttt caagaggagt ctacgcaatt tttggatttt
     4201 atgacaagaa gtctgtaaat accatcacat cattctgtgg gacactccat gtgtccttca
     4261 tcacacctag cttcccaaca gatggcacac atccatttgt catccagatg cgacctgacc
     4321 tcaaaggagc actccttagc ttgattgagt actaccaatg ggacaagttc gcatacctct
     4381 atgacagtga cagaggctta tcaacactgc aagctgttct ggattctgct gcagagaaga
     4441 agtggcaggt gactgctatc aatgtgggga acatcaacaa tgacaagaaa gatgagacct
     4501 acagatcgct ctttcaagat ctggagttaa aaaaagaacg gcgtgtaatc ctggactgtg
     4561 aaagggataa agtaaatgac attgtggacc aggttattac cattggaaaa catgttaaag
     4621 ggtaccatta tatcattgca aatctgggat tcactgatgg ggacctgctg aaaattcagt
     4681 ttggaggagc aaatgtctct ggatttcaga ttgtagacta cgatgattcc ctggtgtcta
     4741 aatttataga aagatggtca acactggaag agaaagaata ccctggagca cacacagcga
     4801 caattaagta tacttcggcc ctgacgtatg atgctgtcca agtgatgact gaagcattcc
     4861 gtaaccttcg gaagcagagg attgaaatat cccggagagg aaatgcaggg gattgtttgg
     4921 ccaacccagc tgtgccctgg ggacaagggg tcgaaataga aagggccctc aagcaggttc
     4981 aagttgaagg cctctctgga aatataaagt ttgaccagaa tggaaaacga ataaactaca
     5041 caattaacat catggagctc aaaacaaatg gaccccggaa gattgggtac tggagtgaag
     5101 tggataaaat ggttgtcacc ctaactgagc tcccatcagg aaatgacacg tctgggcttg
     5161 aaaacaagac tgtggtggtc accacaatat tggaatctcc atatgttatg atgaagaaaa
     5221 atcatgaaat gcttgaaggg aatgagcgtt acgagggcta ctgtgttgac ttagctgcag
     5281 aaattgccaa acactgtggg ttcaagtaca agctgactat tgttggggat ggcaagtatg
     5341 gggccaggga tgccgacacc aaaatttgga atggtatggt tggagagctt gtctacggga
     5401 aagctgacat tgcaattgct ccattaacta tcactctcgt gagagaagag gtgattgact
     5461 tctccaagcc cttcatgagt cttggaatct ctatcatgat caagaagcct cagaagtcca
     5521 aaccaggagt gttttccttt cttgatcctt tagcctatga gatctggatg tgcattgtgt
     5581 ttgcctacat tggggtcagt gtagttttat tcctggtcag cagatttagc ccctacgagt
     5641 ggcacactga ggaatttgaa gatggaagag aaacacaaag tagtgaatca actaatgaat
     5701 ttgggatttt taatagtctc tggttttcct tgggtgcctt tatgcggcaa ggatgcgata
     5761 tttcgccaag atccctctct gggcgcattg ttggaggtgt gtggtggttc tttaccctga
     5821 tcataatctc ctcctacacg gctaacttag ctgccttcct gactgtagag aggatggtgt
     5881 ctcccatcga aagtgctgag gatctgtcta agcaaacaga aattgcttat ggaacattag
     5941 actctggctc cactaaagag tttttcagga gatctaaaat cgcagtgttt gataaaatgt
     6001 ggacttatat gaggagtgca gagccctctg tgtttgtgag gactaccgca gaaggagtag
     6061 ccagagtccg gaaatccaaa ggaaagtatg cctacttgct ggagtccaca atgaacgagt
     6121 acatcgagca gaggaagcct tgtgacacca tgaaagtggg aggaaacttg gattccaaag
     6181 gctacggcat cgccacacct aaaggatcct cattaggaac cccagtaaat cttgcagtat
     6241 tgaaactcag tgagcaaggc gtcttagaca agctgaaaaa caaatggtgg tacgataaag
     6301 gtgaatgtgg agccaaggac tcgggaagta aggaaaagac cagtgccctc agtctgagca
     6361 acgttgctgg agtattctac atccttgtcg ggggccttgg tttggcaatg ctggtggctt
     6421 tgattgagtt ctgttacaag tcaagggccg aggcgaaacg aatgaaggtg gcaaagaatc
     6481 cacagaatat taacccatct tcctcgcaga attcccagaa ttttgcaact tataaggaag
     6541 gttacaacgt atatggcatc gagagtgtta aaatttaggg cgcgccttcg aattaattaa
     6601 ggccgcgact ctagagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     6661 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6721 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6781 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6841 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6901 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6961 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7021 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7081 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7141 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7201 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     7261 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7321 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7381 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7441 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     7501 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     7561 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     7621 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     7681 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     7741 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7801 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7861 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7921 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     7981 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8041 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8101 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8161 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8221 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8281 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8341 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8401 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8461 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8521 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8581 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8641 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     8701 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8761 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8821 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8881 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8941 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9001 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9061 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9121 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9181 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9241 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9301 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9361 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9421 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     9481 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     9541 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9601 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     9661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9721 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     9781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10741 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10801 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10861 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10921 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10981 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11041 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11101 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11161 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11221 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11281 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11341 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11401 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11461 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11521 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11581 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11641 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11701 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11761 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11821 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11881 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    11941 cacctgacac
//
