LOCUS       sequence-330497-        8887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria gene circuit on medium copy backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Zhang R, Goetz H, Melendez-Alvarez J, Li J, Ding T, Wang X, Tian XJ
  TITLE     Winner-takes-all resource competition redirects cascading cell fate 
            transitions.
  JOURNAL   Nat Commun. 2021 Feb 8;12(1):853. doi: 10.1038/s41467-021-21125-3.
  PUBMED    33558556
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-8"; volume: "12(1):853. doi"; pages: " 
            10.1038/s41467-021-21125-3"
COMMENT     Sequence Label: pSB3K3-CT61
FEATURES             Location/Qualifiers
     source          1..8887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             179..892
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             179..892
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(209..237)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     832..852
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             899..931
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      954..1025
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1041..1068
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1221..1232
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1239..2117
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      2139..2210
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2226..2253
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2331..2342
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3123..3194
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3210..3237
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3315..3326
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3333..4007
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4063..4134
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4150..4177
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4255..4266
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4273..5151
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      5173..5244
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5260..5287
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5440..5451
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6037..6069
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      6117..6188
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6204..6231
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6238..6258
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(6398..7213)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6398..7213)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7121..7140
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7205..7224
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7808..8352)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7887..7906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8820..8838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8842..8885)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agacattgat tatttgcacg gcgtcacact ttgctatgcc
       61 atagcaagat agtccataag attagcggat cctacctgac gctttttatc gcaactctct
      121 actgtttctc cataccgttt ttttgggcta gctactagag aaagaggaga aatactagat
      181 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      361 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      481 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtggta cccttgttaa
      541 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      601 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      661 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      721 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      781 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
      841 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc
      901 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1081 agacattgat tatttgcacg gcgtcacact ttgctatgcc atagcaagat agtccataag
     1141 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc cataccgttt
     1201 ttttgggcta gctactagag aaagaggaga aatactagat ggctgaagcg caaaatgatc
     1261 ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg ccgattgagg
     1321 ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt tatattctca
     1381 atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt gtctgccgac
     1441 cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt catccggagg
     1501 ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg catgaatggc
     1561 ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa gcgcaccagc
     1621 cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt
     1681 cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg gaagcgatta
     1741 acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag tacatcagcg
     1801 atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt tgcctgtcgc
     1861 cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta agctggcgcg
     1921 aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg cctatcgcca
     1981 ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta tttaaaaaat
     2041 gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg aatgatgtag
     2101 ccgtcaagtt gtcataataa actactagag ccaggcatca aataaaacga aaggctcagt
     2161 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2221 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     2281 tacagggtta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga
     2341 aatactagat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag
     2401 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt
     2461 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa
     2521 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat
     2581 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg
     2641 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acagcaggtc
     2701 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atacttagtt
     2761 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca
     2821 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata
     2881 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa
     2941 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt
     3001 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa
     3061 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataaactact agagccaggc
     3121 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3181 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     3241 atatactaga gacctgtagg atcgtacagg gttacgcaag aaaatggttt gttatagtcg
     3301 aataaatact agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     3361 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     3421 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     3481 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     3541 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     3601 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     3661 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     3721 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     3781 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     3841 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     3901 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     3961 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgctgct gcaaacgacg
     4021 aaaactacgc agcagcagtt taataatact agagccaggc atcaaataaa acgaaaggct
     4081 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     4141 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gacctgtagg
     4201 atcgtacagg gttacgcaag aaaatggttt gttatagtcg aataaatact agagaaagag
     4261 gagaaatact agatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaac
     4321 gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga tttttttatc
     4381 gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg
     4441 gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct gttcccgcca
     4501 ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt
     4561 tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat
     4621 acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa
     4681 atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt
     4741 gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat
     4801 aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat
     4861 atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca tcttttccgc
     4921 cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag ccaggcgaag
     4981 ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac
     5041 gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc gagcgagttc
     5101 cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata ataaactact
     5161 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     5221 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     5281 ttctgcgttt atatactaga gacattgatt atttgcacgg cgtcacactt tgctatgcca
     5341 tagcaagata gtccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     5401 ctgtttctcc ataccgtttt tttgggctag ctactagaga aagaggagaa atactagatg
     5461 actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt
     5521 ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat
     5581 aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat
     5641 actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa
     5701 agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa
     5761 ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt
     5821 acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat
     5881 gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat
     5941 cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg
     6001 cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct
     6061 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa
     6121 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6181 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     6241 tagtagcggc cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6301 cggcgagcgg tatcagctca ctcaaaggcg gtaatctcga gtcccgtcaa gtcagcgtaa
     6361 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     6421 aatgaaactg caatttattc atatcaggat tatcgatacc atatttttga aaaagccgtt
     6481 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     6541 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     6601 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     6661 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     6721 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     6781 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     6841 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     6901 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     6961 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     7021 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     7081 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     7141 catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac gtttcccgtt
     7201 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     7261 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     7321 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     7381 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     7441 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct
     7501 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgctagcgg agtgtatact
     7561 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     7621 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     7681 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     7741 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     7801 agccgttttt ccataggctc cgccccctga caagcatcac gaaatctgac gctcaaatca
     7861 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     7921 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     7981 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     8041 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     8101 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     8161 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     8221 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     8281 aaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     8341 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaactca
     8401 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8461 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8521 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8581 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8641 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8701 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8761 attccatggt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8821 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     8881 atttctg
//
