LOCUS       sequence-330500-        9776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacteria gene circuit on medium copy backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Zhang R, Goetz H, Melendez-Alvarez J, Li J, Ding T, Wang X, Tian XJ
  TITLE     Winner-takes-all resource competition redirects cascading cell fate 
            transitions.
  JOURNAL   Nat Commun. 2021 Feb 8;12(1):853. doi: 10.1038/s41467-021-21125-3.
  PUBMED    33558556
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-02-8"; volume: "12(1):853. doi"; pages: " 
            10.1038/s41467-021-21125-3"
COMMENT     Sequence Label: pSB3K3-CT81
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             84..704
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(157..176)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             705..737
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      785..856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      872..899
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1050..1061
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1647..1679
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      1727..1798
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1814..1841
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1994..2005
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2012..2725
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2012..2725
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGGTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2042..2070)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2665..2685
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2732..2764
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      2787..2858
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2874..2901
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             3054..3065
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3072..3950
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      3972..4043
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4059..4086
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4164..4175
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4956..5027
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5043..5070
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             5148..5159
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5166..5840
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      5896..5967
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5983..6010
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             6088..6099
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6106..6984
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      7006..7077
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7093..7120
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    7127..7147
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(7287..8102)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7287..8102)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8010..8029
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8094..8113
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(8697..9241)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8776..8795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9709..9727
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9731..9774)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agttgacagc tagctcagtc ctagggacta tgctagctac
       61 tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      301 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      721 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagacattga ttatttgcac ggcgtcacac tttgctatgc catagcaaga
      961 tagtccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     1021 ccattcccta tcagtgatag atactagaga aagaggagaa atactagatg actataatga
     1081 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc
     1141 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat
     1201 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg
     1261 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc
     1321 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt
     1381 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac
     1441 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa
     1501 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag
     1561 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg
     1621 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt
     1681 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1801 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacatt
     1861 gattatttgc acggcgtcac actttgctat gccatagcaa gatagtccat aagattagcg
     1921 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg
     1981 ctagctacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     2041 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     2101 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     2161 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     2221 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     2281 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     2341 tgaagtcaag tttgaaggtg gtacccttgt taatagaatc gagttaaaag gtattgattt
     2401 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     2461 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     2521 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2581 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     2641 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     2701 acatggcatg gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt
     2761 agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2821 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2881 cttcgggtgg gcctttctgc gtttatatac tagagacatt gattatttgc acggcgtcac
     2941 actttgctat gccatagcaa gatagtccat aagattagcg gatcctacct gacgcttttt
     3001 atcgcaactc tctactgttt ctccataccg tttttttggg ctagctacta gagaaagagg
     3061 agaaatacta gatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaacg
     3121 cccatctggt ggcgggttta acgccgattg aggccaatgg ttatctcgat ttttttatcg
     3181 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg
     3241 tgaaaaatca gggacgagaa tttgtctgcc gaccgggtga tattttgctg ttcccgccag
     3301 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt
     3361 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata
     3421 cgggtttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa
     3481 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg
     3541 agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata
     3601 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata
     3661 tcgccagcgt cgcacagcat gtttgcctgt cgccgtcgcg tctgtcacat cttttccgcc
     3721 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgcatcagc caggcgaagc
     3781 tgcttttgag cactacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg
     3841 atcaactcta tttctcgcga gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc
     3901 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa taaactacta
     3961 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     4021 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     4081 tctgcgttta tatactagag acctgtagga tcgtacaggg ttacgcaaga aaatggtttg
     4141 ttatagtcga ataaatacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg
     4201 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc
     4261 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt
     4321 atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga
     4381 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact
     4441 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc
     4501 caaatgtaat taaagaagcg aaaacagcag gtcttatcac tgggtttagt ttccctattc
     4561 atacggctaa caatggcttc ggaatactta gttttgcaca ttcagaaaaa gacaactata
     4621 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg
     4681 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag
     4741 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat
     4801 taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata
     4861 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat
     4921 actttaaaaa ttaataaact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     4981 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     5041 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
     5101 agggttacgc aagaaaatgg tttgttatag tcgaataaat actagagaaa gaggagaaat
     5161 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     5221 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     5281 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     5341 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     5401 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     5461 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     5521 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     5581 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     5641 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     5701 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     5761 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     5821 gtcgtcactc caccggtgct gctgcaaacg acgaaaacta cgcagcagca gtttaataat
     5881 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     5941 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     6001 cctttctgcg tttatatact agagacctgt aggatcgtac agggttacgc aagaaaatgg
     6061 tttgttatag tcgaataaat actagagaaa gaggagaaat actagatggc tgaagcgcaa
     6121 aatgatcccc tgctgccggg atactcgttt aacgcccatc tggtggcggg tttaacgccg
     6181 attgaggcca atggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat
     6241 attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg agaatttgtc
     6301 tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta cggtcgtcat
     6361 ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc ctactggcat
     6421 gaatggctta actggccgtc aatatttgcc aatacgggtt tctttcgccc ggatgaagcg
     6481 caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca aggggaaggg
     6541 cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg gcgcatggaa
     6601 gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc ttgtcagtac
     6661 atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc
     6721 ctgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc
     6781 tggcgcgagg accaacgcat cagccaggcg aagctgcttt tgagcactac ccggatgcct
     6841 atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc gcgagtattt
     6901 aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat
     6961 gatgtagccg tcaagttgtc ataataaact actagagcca ggcatcaaat aaaacgaaag
     7021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     7081 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agtagcggcc
     7141 gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7201 atcagctcac tcaaaggcgg taatctcgag tcccgtcaag tcagcgtaat gctctgccag
     7261 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     7321 aatttattca tatcaggatt atcgatacca tatttttgaa aaagccgttt ctgtaatgaa
     7381 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     7441 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     7501 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     7561 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     7621 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     7681 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     7741 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     7801 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     7861 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     7921 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     7981 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     8041 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
     8101 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     8161 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     8221 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
     8281 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
     8341 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
     8401 gcaacacctt cttcacgagg cagacctcag cgctagcgga gtgtatactg gcttactatg
     8461 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
     8521 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
     8581 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     8641 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     8701 cataggctcc gccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     8761 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     8821 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     8881 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     8941 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9001 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     9061 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     9121 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttca aaaaaccgcc
     9181 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     9241 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttccatggtg
     9661 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9721 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctg
//
