LOCUS       sequence-33051-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_mCherry
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(150..170)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(174..194)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(233..943)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPXPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(511..530)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     780..798
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      940..949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgaacccttg cgtcgacact agttctagac cctcacttcc tactcaggct ttattcaaag
       61 accaagaggt acaggtgcaa gggagagaag aagggcatgg ctagaaggca agccccgcag
      121 aaggcagctt atctagatcc ggtggatcct acctttctct tcttttttgg atctaccttt
      181 ctcttctttt ttggatctac cttctcttct tttttggatc agctcgagat ctttacttgt
      241 acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga
      301 tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg
      361 gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc
      421 cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca
      481 tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg
      541 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
      601 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      661 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      721 tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcang gggccaccct
      781 tggtcacctt cagcttggcg gtctgggtgc cctcgtangg gcggccctcg ccctcgccct
      841 cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact
      901 ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catggtggct c
//
