LOCUS       sequence-330529-        4268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression vector for Escherichia coli. BglBrick 
            compatible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, 
            Breitling R, Takano E, Scrutton NS
  TITLE     SelProm: A Queryable and Predictive Expression Vector Selection Tool
            for Escherichia coli.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399.
  PUBMED    30870592
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399."
COMMENT     Sequence Label: pBbS20a-RFP
FEATURES             Location/Qualifiers
     source          1..4268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     CDS             81..758
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      791..862
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      878..905
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1436..2386)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3148..3242)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3272..4063,4064..4132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3895..3914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4133..4237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttatagcta gctcagtcct aggtacaatg ctagcaggtt taatcgaatt caaaagatct
       61 tttaagaagg agatatacat atggcgagta gcgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaagg atccaaactc gagtaaggat
      781 ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      841 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      901 ctgcgtttat acctagggta cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct
      961 agtttgttat cagaatcgca gatccggctt cagccggttt gccggctgaa agcgctattt
     1021 cttccagaat tgccatgatt ttttccccac gggaggcgtc actggctccc gtgttgtcgg
     1081 cagctttgat tcgataagca gcatcgcctg tttcaggctg tctatgtgtg actgttgagc
     1141 tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt tactggtttc
     1201 acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct gttcatggtg
     1261 aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc
     1321 gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct
     1381 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga
     1441 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg
     1501 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac
     1561 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta
     1621 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt
     1681 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat
     1741 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt
     1801 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa
     1861 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct
     1921 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag
     1981 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata
     2041 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact
     2101 ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct
     2161 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag
     2221 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg
     2281 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc
     2341 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt
     2401 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc
     2461 ttttcccggg tgatctgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa
     2521 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc
     2581 aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca
     2641 gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa
     2701 cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg
     2761 caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca cctgagtcgc
     2821 tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa
     2881 tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag
     2941 cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt
     3001 tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg
     3061 tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt
     3121 acccgtctta ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     3181 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     3241 tccaagcgag ctcgtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4261 ctgacgtc
//
