LOCUS       sequence-330536-       11791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of 
            HER2+TM(23-675)-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Hernandez-Lopez RA, Yu W, Cabral KA, Creasey OA, Lopez Pazmino MDP, 
            Tonai Y, De Guzman A, Makela A, Saksela K, Gartner ZJ, Lim WA
  TITLE     T cell circuits that sense antigen density with an ultrasensitive 
            threshold.
  JOURNAL   Science. 2021 Mar 12;371(6534):1166-1171. doi: 
            10.1126/science.abc1855. Epub 2021 Feb 25.
  PUBMED    33632893
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc1855"; journalName: "Science"; date: 
            "2021-03-12- 12"; volume: "371"; issue: "6534"; pages: "1166-1171"
COMMENT     Sequence Label: pHR_SFFv_Her2-BFP_RHL027
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2792..2801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4844..5545
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5655..6243
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5708..5728)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6126..6137)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6246..6262)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6247..6263)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6353..6586
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6611..6629)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6612..6629)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6919..6938)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7152..7171)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7271..7293
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7331..7349)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7417..7521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7522..7590,7591..8382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7740..7759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8553..9141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9042..9061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9295..9312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9382..9402)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9387..9716
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9567..9702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10850..10915
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11045..11065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11490..11624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11527..11546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11581..11600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
     2761 caagtggagc aaggcaggtg gacagtggat cgccgccatg gccctgccag tcacagctct
     2821 gttgttgccc ctggctctgt tgctccatgc cgcgcgaccc acgcaagtat gcacgggaac
     2881 cgacatgaaa ctgcgcctgc cggcttcacc ggagacacat ttggatatgt tgcgccacct
     2941 ttatcagggc tgtcaagttg tgcaggggaa tttggaactc acgtaccttc ccactaatgc
     3001 ctcactcagt tttctgcagg acatccagga ggtgcagggt tacgttctga ttgcccacaa
     3061 ccaagtacgc caggtgccac tgcaaaggtt gagaatcgtg agaggcacac agctgtttga
     3121 agataactat gctctcgctg ttcttgacaa cggggaccct ttgaacaata ctactccggt
     3181 tacaggggcg tctcccggcg gccttaggga gctgcagttg cgctctctta ccgagatatt
     3241 gaaaggcggc gtactgatcc aacggaatcc ccaactctgc tatcaggaca ccatcctgtg
     3301 gaaggatatt ttccacaaga ataatcagct cgcgctgact ctcatagaca caaatcggag
     3361 ccgggcatgt cacccatgta gcccaatgtg caaaggttcc cgatgctggg gtgaatcaag
     3421 tgaagattgc caatccttga ctcgaaccgt gtgtgctggt ggttgtgcac gatgcaaagg
     3481 gcctcttcct acggattgtt gtcatgaaca atgcgctgct gggtgtactg gacctaaaca
     3541 tagtgactgc ctggcgtgcc tgcacttcaa tcattctgga atatgtgaat tgcactgtcc
     3601 agcgcttgta acatataaca cggacacatt tgagtcaatg ccaaatcccg aaggcagata
     3661 cactttcggg gccagttgtg tgacagcctg cccctacaac tacctgagta ccgacgtggg
     3721 gtcctgcacc ctcgtttgcc ctttgcacaa ccaggaggtc acagcggagg acggtacaca
     3781 gcgatgtgag aagtgtagca agccgtgcgc gcgagtctgt tacggcttgg gaatggagca
     3841 tttgcgggag gttagagcgg ttacctctgc gaatattcaa gagtttgcag gttgtaaaaa
     3901 aatattcggc tccttggcat tcctgcccga gagtttcgac ggagatcctg cttcaaacac
     3961 cgctcccctc caacctgagc aacttcaagt ctttgaaaca cttgaagaga ttacgggtta
     4021 cctctatata tccgcgtggc cggactctct ccctgacttg tcagtgtttc aaaatcttca
     4081 ggtgattagg gggcggattc ttcataatgg agcctattca cttactcttc agggcttggg
     4141 catttcctgg ctcggcctca gatcattgag agagcttggt tcaggacttg ctctgataca
     4201 tcacaacact cacctgtgtt ttgttcacac agttccatgg gatcaacttt tccgaaatcc
     4261 ccaccaggcc ttgctgcaca ctgccaatcg cccagaggat gagtgtgtgg gggagggact
     4321 cgcgtgccat cagttgtgtg cgagaggtca ctgttggggg ccaggcccga cccagtgtgt
     4381 aaactgcagc cagtttttga gagggcagga atgcgttgaa gagtgcagag ttcttcaggg
     4441 gctgccaagg gagtatgtga acgcgcgaca ttgtctccca tgccaccctg agtgccagcc
     4501 gcaaaacggc agcgtgacat gtttcggacc cgaggctgac cagtgtgttg cctgcgcaca
     4561 ttacaaggat cccccgtttt gtgtcgcccg ctgcccttca ggcgtgaaac cagacctgtc
     4621 ctacatgcct atatggaaat tccctgatga ggagggcgca tgccaacctt gtccgatcaa
     4681 ctgcactcac agttgcgtag atctggacga taagggctgt cctgcagagc aacgcgcttc
     4741 cccgttgacg tcaattatat cagccgtggt tggcattctt cttgtagttg ttttgggtgt
     4801 agtattcggc atactcatcg gaagcgggtc cggtagcgga tctatgagcg agctgattaa
     4861 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     4921 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     4981 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     5041 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     5101 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     5161 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     5221 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     5281 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     5341 cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa
     5401 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     5461 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     5521 caaactgggg cacaagctta attaggatcc ttgacttgcg gccgcaactc ccacctgcaa
     5581 catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata
     5641 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5701 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5761 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5821 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5881 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5941 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6001 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6061 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6121 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6181 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6241 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
     6301 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6361 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     6601 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     6661 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     6721 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     6781 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     6841 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     6901 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     6961 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     7021 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     7081 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     7141 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7201 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7261 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7321 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7381 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7501 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7561 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7621 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7681 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7741 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7801 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7861 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7921 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7981 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8041 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8101 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8161 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8221 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8281 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8341 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8401 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8461 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8521 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8581 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8641 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8701 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8761 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8821 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8881 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8941 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9001 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9061 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9121 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9181 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9241 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9301 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9361 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9421 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9481 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9541 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9601 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9661 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9721 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     9781 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     9841 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     9901 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     9961 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10021 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
    10081 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
    10141 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
    10201 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
    10261 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
    10321 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
    10381 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
    10441 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
    10501 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
    10561 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
    10621 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
    10681 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
    10741 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
    10801 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
    10861 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
    10921 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
    10981 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
    11041 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
    11101 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
    11161 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
    11221 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
    11281 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
    11341 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
    11401 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    11461 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    11521 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    11581 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    11641 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    11701 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11761 tttgttaaat atgtactaca aacttagtag t
//
