LOCUS       sequence-33055-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     Christopher Potter Lab Plasmids
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pXL-BACII-LoxP-3xPDsRed-LoxP
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(439..472)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   657..719
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(849..866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(849..865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(858..880)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg ccctcgccct
       61 cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag cgcatgaact
      121 cggtgatgac gttctcggag gaggccatgg tggcgaccgg tggatcccgg gcccgcggta
      181 ccccgattgt ttagcttgtt cagctgcgct tgtttatttg cttagctttc gcttagcgac
      241 gtgttcactt tgcttgtttg aattgaattg tcgctccgta gacgaagcgc ctctatttat
      301 actccggcgg tcgagggttc gaaatcgata agcttggatc ctaattgaat tagctctaat
      361 tgaattagtc tctaattgaa ttagatcccc gggcgagctc gaattaacca ttgtgggaac
      421 actagaacta gtggatccat aacttcgtat agcatacatt atacgaagtt atatgaattc
      481 gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt taataaataa
      541 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
      601 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
      661 cgtcaatttt acacatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
      721 taatctagct gcgtgttctg cagcgtgtcg agcatcttca tctgctccat cacgctgtaa
      781 aacacatttg caccgcgagt ctgcccgtcc tccacgggtt caaaaacgtg aatgaacgag
      841 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
//
