LOCUS       sequence-33057-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP116
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..56)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        57..338
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 tcgactctag aggatccccg ggataacttc gtatagcata cattatacga agttataacg
       61 acattactat atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta
      121 ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt
      181 gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt
      241 cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt
      301 gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag
      361 gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg
      421 ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa
      481 ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag
      541 gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc
      601 tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat
      661 accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca
      721 tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag
      781 caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag
      841 agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg
      901 atgcgcttag a
//
