LOCUS       sequence-33058-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP118
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..57
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtataatgt atgctatacg aagttatggg
       61 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
      121 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
      181 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
      241 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc
      301 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc
      361 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct
      421 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
      481 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt
      541 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc
      601 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa
      661 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac
      721 tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
      781 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc
      841 acacaagttt gtttgctttt cgtgcatgat att
//
