LOCUS       sequence-330584-       10350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human TNPO3 fused to eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, 
            Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
  TITLE     Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection 
            in a Cyclophilin A-Dependent Manner.
  JOURNAL   mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 
            10.1128/mBio.03142-20.
  PUBMED    33758083
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-23"; volume: "12(2). pii: mBio.03142-20. doi"; pages:
            " 10.1128/mBio.03142-20"
COMMENT     Sequence Label: pSICO-eGFP-TNPO3
FEATURES             Location/Qualifiers
     source          1..10350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2614..2634
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2652..2685)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        2785..3088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3089..3292
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3331..3340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3337..3339,3340..3342,3343..4053)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3337..3339,3340..3342,3343..4053)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3382..3403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3643..3662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3990..4011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4096..4122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     6911..6927
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7002..7590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7055..7075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7473..7484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7593..7609)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8119..8299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8361..8949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8441..8460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9120..9911,9912..9980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9743..9762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9981..10085)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10160..10179)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gagatccgac gcgccatctc
     2401 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc
     2461 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac
     2521 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa
     2581 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact
     2641 gtaaagtaat tataacttcg tatagtataa attatacgaa gttataagcc ttggtttttt
     2701 gaattccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     2761 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2821 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     2881 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2941 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3001 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3061 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3121 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3181 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3241 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3301 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3361 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3421 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3541 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3601 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3661 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3721 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3781 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3841 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3901 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3961 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4021 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
     4081 tcgaattcca ccatgtaccc atacgacgtc ccagactacg ctatggaagg agcaaagccg
     4141 acattgcagc tcgtgtacca ggcagtgcag gcgctttacc acgacccaga tcccagcgga
     4201 aaggagcgcg cctctttttg gcttggggag ctgcagcgtt cggttcatgc atgggagatc
     4261 tcagaccagt tgttacagat ccggcaggat gtggagtcat gctattttgc tgcacagacc
     4321 atgaaaatga agattcagac ctcattttat gagctcccca cagactctca tgcctcttta
     4381 cgggactcat tgctaaccca tatccagaac ttgaaagact tgtcacctgt tattgtaacg
     4441 cagctggctt tagcaatagc agatcttgcc ctacagatgc cttcctggaa gggatgtgtg
     4501 caaacactgg tggaaaaata cagcaatgat gtgacttctt tgcctttttt gctggagatc
     4561 cttacagtgt tacctgaaga agtacatagt cgttccttac gaattggagc taatcggcgc
     4621 acagaaatta tagaagattt ggccttctac tctagtacag tagtatctct attgatgacc
     4681 tgtgtagaaa aagcaggaac agatgagaaa atgcttatga aggtttttcg ctgtttggga
     4741 agttggttta acttgggagt tttggacagt aacttcatgg ctaacaataa attactagca
     4801 ctcctttttg aggttttgca acaggataag acctcgtcta acctacatga agctgcttcg
     4861 gactgtgtat gctcagctct ctacgccatt gagaatgtgg agactaactt gccattagcc
     4921 atgcaacttt ttcagggagt gctgacattg gagactgcct atcatatggc cgtggcacgt
     4981 gaagatttag acaaagttct gaattactgc cgtattttca ctgaactatg tgaaactttt
     5041 cttgaaaaaa ttgtttgtac tccaggccaa ggtcttgggg accttcgaac tctggagctg
     5101 ctgcttatct gtgcaggcca tcctcaatat gaggtagtag aaatttcatt taacttttgg
     5161 taccgactgg ggaaacattt gtacaaaact aacgatgaag ttattcatgg catcttcaaa
     5221 gcttacattc agaggctgct tcacgccttg gctcgacact gccagctgga accagaccat
     5281 gagggggttc ctgaggagac tgatgacttt ggggagtttc gcatgagggt atcagacctg
     5341 gtaaaggact tgattttctt gatagggtct atggagtgtt ttgctcagtt atattctact
     5401 ctgaaagaag gcaacccacc ctgggaggtg acagaagcgg ttctctttat catggctgct
     5461 atagcaaaga gtgttgatcc ggaaaacaat ccaacacttg tggaagtcct agaaggagtt
     5521 gtccgcctcc cggagaccgt acatacggct gtgcgataca ccagcattga attggttgga
     5581 gagatgagtg aagtcgttga tcgaaatcct cagttccttg accctgtgtt gggctatttg
     5641 atgaaaggcc tgtgtgaaaa gcccctggct tctgctgcag ccaaagccat tcataacatt
     5701 tgctctgtct gccgagatca catggctcag cactttaatg gactcctgga gattgcccgc
     5761 tccctcgatt ccttcctgtt gtctccagaa gctgctgtgg gcttgctaaa agggacagca
     5821 cttgtcctag cccgattacc tttggataag attaccgaat gtcttagtga actatgttct
     5881 gttcaggtta tggcattgaa aaagctgttg tctcaagagc ccagcaatgg catatcctca
     5941 gatcccacag tgttcttaga tcgccttgca gtgatattta ggcataccaa tcccattgtg
     6001 gaaaatggac agactcatcc gtgtcagaaa gtcatacagg aaatatggcc agttttatcc
     6061 gagactctaa ataagcaccg tgcagacaac cgcatcgtag agcgttgttg caggtgcctg
     6121 cgctttgctg ttcgctgtgt aggcaaagga tctgcagcac tgctgcagcc actagtcaca
     6181 cagatggtga atgtgtacca cgtacatcag cattcctgct tcctgtacct tggcagtatc
     6241 cttgtggatg aatatggcat ggaagaaggc tgtcggcagg gactgctaga catgctccag
     6301 gcactgtgca tccccacctt tcagctccta gaacagcaga atggtctcca gaatcaccat
     6361 gacactgtag atgacctgtt ccggctagcc acaaggttta ttcagcgtag ccctgtcacc
     6421 ttgctgcgga gccaagtggt catccctatc ttacagtggg ccattgcctc tactaccctg
     6481 gaccaccggg atgccaattg tagtgtcatg aggtttctac gagacctcat tcatacaggg
     6541 gtagccaatg atcatgaaga agactttgaa ttacggaaag aactgattgg acaggtgatg
     6601 aaccagcttg gacagcagct tgtcagccag ctgctgcaca cctgctgctt ttgcctcccc
     6661 ccctataccc taccagatgt ggctgaagtg ctctggggga tcatgcaggt tgacagaccg
     6721 actttttgtc gatggttaga aaattcctta aaaggtttgc caaaggaaac aaccgtggga
     6781 gccgtcacag tgacacacaa acaacttaca gacttccaca agcaagtcac tagtgctgag
     6841 gaatgtaaac aagtttgctg ggccttgcga gacttcacca ggttgtttcg atagggatcc
     6901 cggtgaccct cgaggtcgac ggtatcgata agctcgcttc acgagatcat gtttaagggt
     6961 tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca
     7021 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7081 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7141 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7201 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7261 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7321 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7381 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7441 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7501 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7561 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta
     7621 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     7681 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     7741 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7801 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     7861 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     7921 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     7981 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     8041 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     8101 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8161 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8221 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8281 gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10081 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    10141 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    10201 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    10261 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    10321 ttcgcgatgt acgggccaga tatacgcgtt
//
