LOCUS       sequence-33059-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP120
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..57
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(259..921)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtataatgt atgctatacg aagttattcg
       61 agttcaagag aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga
      121 atgacacgta tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca
      181 tacttactga cattcatagg tatacatata tacacatgta tatatatcgt atgctgcagc
      241 tttaaataat cggtgtcact acataagaac acctttggtg gagggaacat cgttggtacc
      301 attgggcgag gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac tctcactacg
      361 gtgatgatca ttcttgcctc gcagacaatc aacgtggagg gtaattctgc tagcctctgc
      421 aaagctttca agaaaatgcg ggatcatctc gcaagagaga tctcctactt tctccctttg
      481 caaaccaagt tcgacaactg cgtacggcct gttcgaaaga tctaccaccg ctctggaaag
      541 tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt actccacgca cggcccctag
      601 ggcctcttta aaagcttgac cgagagcaat cccgcagtct tcagtggtgt gatggtcgtc
      661 tatgtgtaag tcaccaatgc actcaacgat tagcgaccag ccggaatgct tggccagagc
      721 atgtatcata tggtccagaa accctatacc tgtgtggacg ttaatcactt gcgattgtgt
      781 ggcctgttct gctactgctt ctgcctcttt ttctgggaag atcgagtgct ctatcgctag
      841 gggaccaccc tttaaagaga tcgcaatctg aatcttggtt tcatttgtaa tacgctttac
      901 tagggctttc tgctctgtca tc
//
