LOCUS       sequence-330594-       12874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses halo tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_Halo_GluA2
FEATURES             Location/Qualifiers
     source          1..12874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2580..3027
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3072..3092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3162..3183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3423..3442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3770..3791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3843..3899
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4002..4892
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    7564..8152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7617..7637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8035..8046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8155..8171)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8156..8172)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8677..8857
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8883..8900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8889..9113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9159..9587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9246..9265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9456..9477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9596..9616)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9601..9930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9781..9916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9843..9862
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9978..10025
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10044..10418
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10548..10669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10585..10604)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10639..10658
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10718..10734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10718..10734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10731..10753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10742..10758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10766..10772,10773..10790,10791..10796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10811..10832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10949..10966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11120..11708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11200..11219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11879..12670,12671..12739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12502..12521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12740..12844)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta atcgcatgct agagagggcc ctgcgtatga
     2581 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     2641 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     2701 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     2761 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     2821 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     2881 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     2941 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     3001 ggagtcgtgt cgtgcctgag agcgcagtcg gatccgccac catggctcca aaaaagaaga
     3061 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatgg
     3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3301 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     3841 gagctactaa cttcagcctg ctgaagcaag caggagatgt tgaagaaaac cccgggcctg
     3901 ctagcatgca aaagattatg catatttctg tcctcctttc tcctgtttta tggggactga
     3961 tttttggtgt ctcttctggt ggctctggcg gaactggagg tatggcagaa atcggtactg
     4021 gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac tacgtcgatg
     4081 ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc tcctcctacg
     4141 tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct ccagacctga
     4201 tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac cacgtccgct
     4261 tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc attcacgact
     4321 ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc aaaggtattg
     4381 catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa tttgcccgcg
     4441 agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc gatcagaacg
     4501 tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa gtcgagatgg
     4561 accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg cgcttcccaa
     4621 acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa gaatacatgg
     4681 actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca ggcgttctga
     4741 tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag gctgtggaca
     4801 tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc agcgagatcg
     4861 cgcgctggct gtcgacgctc gagatttccg gcggcactgg aggtagcggt ggtagcggcg
     4921 gtaacagcat acagataggg gggctatttc caaggggcgc tgatcaagaa tacagtgcat
     4981 ttcgggtagg gatggttcag ttttccactt cggagttcag actgacaccc catatcgaca
     5041 atttggaggt agccaacagt ttcgcagtca ccaatgcttt ctgctcccag ttttcaagag
     5101 gagtctacgc aatttttgga ttttatgaca agaagtctgt aaataccatc acatcattct
     5161 gtgggacact ccatgtgtcc ttcatcacac ctagcttccc aacagatggc acacatccat
     5221 ttgtcatcca gatgcgacct gacctcaaag gagcactcct tagcttgatt gagtactacc
     5281 aatgggacaa gttcgcatac ctctatgaca gtgacagagg cttatcaaca ctgcaagctg
     5341 ttctggattc tgctgcagag aagaagtggc aggtgactgc tatcaatgtg gggaacatca
     5401 acaatgacaa gaaagatgag acctacagat cgctctttca agatctggag ttaaaaaaag
     5461 aacggcgtgt aatcctggac tgtgaaaggg ataaagtaaa tgacattgtg gaccaggtta
     5521 ttaccattgg aaaacatgtt aaagggtacc attatatcat tgcaaatctg ggattcactg
     5581 atggggacct gctgaaaatt cagtttggag gagcaaatgt ctctggattt cagattgtag
     5641 actacgatga ttccctggtg tctaaattta tagaaagatg gtcaacactg gaagagaaag
     5701 aataccctgg agcacacaca gcgacaatta agtatacttc ggccctgacg tatgatgctg
     5761 tccaagtgat gactgaagca ttccgtaacc ttcggaagca gaggattgaa atatcccgga
     5821 gaggaaatgc aggggattgt ttggccaacc cagctgtgcc ctggggacaa ggggtcgaaa
     5881 tagaaagggc cctcaagcag gttcaagttg aaggcctctc tggaaatata aagtttgacc
     5941 agaatggaaa acgaataaac tacacaatta acatcatgga gctcaaaaca aatggacccc
     6001 ggaagattgg gtactggagt gaagtggata aaatggttgt caccctaact gagctcccat
     6061 caggaaatga cacgtctggg cttgaaaaca agactgtggt ggtcaccaca atattggaat
     6121 ctccatatgt tatgatgaag aaaaatcatg aaatgcttga agggaatgag cgttacgagg
     6181 gctactgtgt tgacttagct gcagaaattg ccaaacactg tgggttcaag tacaagctga
     6241 ctattgttgg ggatggcaag tatggggcca gggatgccga caccaaaatt tggaatggta
     6301 tggttggaga gcttgtctac gggaaagctg acattgcaat tgctccatta actatcactc
     6361 tcgtgagaga agaggtgatt gacttctcca agcccttcat gagtcttgga atctctatca
     6421 tgatcaagaa gcctcagaag tccaaaccag gagtgttttc ctttcttgat cctttagcct
     6481 atgagatctg gatgtgcatt gtgtttgcct acattggggt cagtgtagtt ttattcctgg
     6541 tcagcagatt tagcccctac gagtggcaca ctgaggaatt tgaagatgga agagaaacac
     6601 aaagtagtga atcaactaat gaatttggga tttttaatag tctctggttt tccttgggtg
     6661 cctttatgcg gcaaggatgc gatatttcgc caagatccct ctctgggcgc attgttggag
     6721 gtgtgtggtg gttctttacc ctgatcataa tctcctccta cacggctaac ttagctgcct
     6781 tcctgactgt agagaggatg gtgtctccca tcgaaagtgc tgaggatctg tctaagcaaa
     6841 cagaaattgc ttatggaaca ttagactctg gctccactaa agagtttttc aggagatcta
     6901 aaatcgcagt gtttgataaa atgtggactt atatgaggag tgcagagccc tctgtgtttg
     6961 tgaggactac cgcagaagga gtagccagag tccggaaatc caaaggaaag tatgcctact
     7021 tgctggagtc cacaatgaac gagtacatcg agcagaggaa gccttgtgac accatgaaag
     7081 tgggaggaaa cttggattcc aaaggctacg gcatcgccac acctaaagga tcctcattag
     7141 gaaccccagt aaatcttgca gtattgaaac tcagtgagca aggcgtctta gacaagctga
     7201 aaaacaaatg gtggtacgat aaaggtgaat gtggagccaa ggactcggga agtaaggaaa
     7261 agaccagtgc cctcagtctg agcaacgttg ctggagtatt ctacatcctt gtcgggggcc
     7321 ttggtttggc aatgctggtg gctttgattg agttctgtta caagtcaagg gccgaggcga
     7381 aacgaatgaa ggtggcaaag aatccacaga atattaaccc atcttcctcg cagaattccc
     7441 agaattttgc aacttataag gaaggttaca acgtatatgg catcgagagt gttaaaattt
     7501 agggcgcgcc ttcgaattaa ttaaggccgc gactctagag aattcgatat caagcttatc
     7561 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7921 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     8161 cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     8221 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     8281 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8341 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     8401 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     8461 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
     8521 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
     8581 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
     8641 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
     8701 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8761 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8821 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     8881 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8941 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     9001 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     9061 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     9121 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     9181 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9241 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9301 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9361 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9421 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9481 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     9601 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9661 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9721 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     9781 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9841 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     9901 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     9961 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    10021 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    10081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    10141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    10201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    10261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    10321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    10381 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    10441 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    10501 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    10561 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10621 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    10681 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    10741 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10801 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10861 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10921 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    11041 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11281 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11521 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11761 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11821 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11881 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11941 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    12001 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    12061 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    12121 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12181 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12241 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12301 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12361 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12421 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12481 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12541 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12601 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12661 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12721 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12781 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12841 cgcgcacatt tccccgaaaa gtgccacctg acac
//
