LOCUS       sequence-330595-       12490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNAP tagged GluA2 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP-P2A_SNAP_GluA2
FEATURES             Location/Qualifiers
     source          1..12490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2580..3027
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3072..3092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3162..3183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3423..3442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3770..3791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3843..3899
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7180..7768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7233..7253)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7651..7662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7771..7787)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7772..7788)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8293..8473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8499..8516)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8505..8729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8775..9203
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8862..8881)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9072..9093
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9212..9232)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9217..9546
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9397..9532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9459..9478
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9594..9641
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9660..10034
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10164..10285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10201..10220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10255..10274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10334..10350)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10334..10350)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10347..10369)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10358..10374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10382..10388,10389..10406,10407..10412))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10427..10448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10565..10582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10736..11324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10816..10835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11495..12286,12287..12355))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12118..12137
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12356..12460)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta atcgcatgct agagagggcc ctgcgtatga
     2581 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     2641 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     2701 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     2761 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     2821 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     2881 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     2941 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     3001 ggagtcgtgt cgtgcctgag agcgcagtcg gatccgccac catggctcca aaaaagaaga
     3061 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatgg
     3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3301 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     3841 gagctactaa cttcagcctg ctgaagcaag caggagatgt tgaagaaaac cccgggcctg
     3901 ctagcatgca aaagattatg catatttctg tcctcctttc tcctgtttta tggggactga
     3961 tttttggtgt ctcttctatg ggcctgggct ccatggacaa ggattgtgaa atgaaacgca
     4021 ccacactgga cagccctttg gggaagctgg agctgtctgg ttgtgagcag ggtctgcacg
     4081 aaataatttt tctgggcaag gggacgtctg cagctgatgc cgtggaggtc ccagcccccg
     4141 ctgcggttct cggaggtccg gagcccctga tgcaggctac agcctggctg aatgcctatt
     4201 tccaccagcc cgaggctatc gaagagttcc ccgtgccggc tcttcaccat cccgttttcc
     4261 agcaagagtc gttcaccaga caggtgttat ggaagctgct gaaggttgtg aaattcggag
     4321 aagtgatttc ttactctcac ttagcagccc tggcaggcaa ccccgcagcc acggcagcag
     4381 tgaagacggc actgagtggc aatcctgtcc ctatcctgat cccgtgccac agagtggtcc
     4441 agggggattt ggatgtgggc ggttacgagg gtggactggc cgtgaaggaa tggcttctgg
     4501 cccatgaagg ccaccggttg gggaagccag gcttgggaaa cagcatacag ataggaggcc
     4561 tatttccaag gggcgctgat caagaataca gtgcatttcg ggtagggatg gttcagtttt
     4621 ccacttcgga gttcagactg acaccccata tcgacaattt ggaggtagcc aacagtttcg
     4681 cagtcaccaa tgctttctgc tcccagtttt caagaggagt ctacgcaatt tttggatttt
     4741 atgacaagaa gtctgtaaat accatcacat cattctgtgg gacactccat gtgtccttca
     4801 tcacacctag cttcccaaca gatggcacac atccatttgt catccagatg cgacctgacc
     4861 tcaaaggagc actccttagc ttgattgagt actaccaatg ggacaagttc gcatacctct
     4921 atgacagtga cagaggctta tcaacactgc aagctgttct ggattctgct gcagagaaga
     4981 agtggcaggt gactgctatc aatgtgggga acatcaacaa tgacaagaaa gatgagacct
     5041 acagatcgct ctttcaagat ctggagttaa aaaaagaacg gcgtgtaatc ctggactgtg
     5101 aaagggataa agtaaatgac attgtggacc aggttattac cattggaaaa catgttaaag
     5161 ggtaccatta tatcattgca aatctgggat tcactgatgg ggacctgctg aaaattcagt
     5221 ttggaggagc aaatgtctct ggatttcaga ttgtagacta cgatgattcc ctggtgtcta
     5281 aatttataga aagatggtca acactggaag agaaagaata ccctggagca cacacagcga
     5341 caattaagta tacttcggcc ctgacgtatg atgctgtcca agtgatgact gaagcattcc
     5401 gtaaccttcg gaagcagagg attgaaatat cccggagagg aaatgcaggg gattgtttgg
     5461 ccaacccagc tgtgccctgg ggacaagggg tcgaaataga aagggccctc aagcaggttc
     5521 aagttgaagg cctctctgga aatataaagt ttgaccagaa tggaaaacga ataaactaca
     5581 caattaacat catggagctc aaaacaaatg gaccccggaa gattgggtac tggagtgaag
     5641 tggataaaat ggttgtcacc ctaactgagc tcccatcagg aaatgacacg tctgggcttg
     5701 aaaacaagac tgtggtggtc accacaatat tggaatctcc atatgttatg atgaagaaaa
     5761 atcatgaaat gcttgaaggg aatgagcgtt acgagggcta ctgtgttgac ttagctgcag
     5821 aaattgccaa acactgtggg ttcaagtaca agctgactat tgttggggat ggcaagtatg
     5881 gggccaggga tgccgacacc aaaatttgga atggtatggt tggagagctt gtctacggga
     5941 aagctgacat tgcaattgct ccattaacta tcactctcgt gagagaagag gtgattgact
     6001 tctccaagcc cttcatgagt cttggaatct ctatcatgat caagaagcct cagaagtcca
     6061 aaccaggagt gttttccttt cttgatcctt tagcctatga gatctggatg tgcattgtgt
     6121 ttgcctacat tggggtcagt gtagttttat tcctggtcag cagatttagc ccctacgagt
     6181 ggcacactga ggaatttgaa gatggaagag aaacacaaag tagtgaatca actaatgaat
     6241 ttgggatttt taatagtctc tggttttcct tgggtgcctt tatgcggcaa ggatgcgata
     6301 tttcgccaag atccctctct gggcgcattg ttggaggtgt gtggtggttc tttaccctga
     6361 tcataatctc ctcctacacg gctaacttag ctgccttcct gactgtagag aggatggtgt
     6421 ctcccatcga aagtgctgag gatctgtcta agcaaacaga aattgcttat ggaacattag
     6481 actctggctc cactaaagag tttttcagga gatctaaaat cgcagtgttt gataaaatgt
     6541 ggacttatat gaggagtgca gagccctctg tgtttgtgag gactaccgca gaaggagtag
     6601 ccagagtccg gaaatccaaa ggaaagtatg cctacttgct ggagtccaca atgaacgagt
     6661 acatcgagca gaggaagcct tgtgacacca tgaaagtggg aggaaacttg gattccaaag
     6721 gctacggcat cgccacacct aaaggatcct cattaggaac cccagtaaat cttgcagtat
     6781 tgaaactcag tgagcaaggc gtcttagaca agctgaaaaa caaatggtgg tacgataaag
     6841 gtgaatgtgg agccaaggac tcgggaagta aggaaaagac cagtgccctc agtctgagca
     6901 acgttgctgg agtattctac atccttgtcg ggggccttgg tttggcaatg ctggtggctt
     6961 tgattgagtt ctgttacaag tcaagggccg aggcgaaacg aatgaaggtg gcaaagaatc
     7021 cacagaatat taacccatct tcctcgcaga attcccagaa ttttgcaact tataaggaag
     7081 gttacaacgt atatggcatc gagagtgtta aaatttaggg cgcgccttcg aattaattaa
     7141 ggccgcgact ctagagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     7201 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7261 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7321 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7381 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7441 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7501 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7561 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7621 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7681 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7741 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     7801 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     7861 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     7921 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7981 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8041 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     8101 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     8161 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8221 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8281 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8341 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8401 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8461 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8521 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8581 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8641 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8701 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8761 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8821 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8881 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8941 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9001 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9061 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9121 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9181 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9241 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9301 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9361 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9421 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9481 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9541 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9601 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9661 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9721 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9781 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9841 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9901 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9961 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
    10021 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    10081 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10141 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10261 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10321 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10381 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10441 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10501 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11161 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12481 cacctgacac
//
