LOCUS       sequence-330599-       12124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histidine tagged NR1 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_His6_NR1.
FEATURES             Location/Qualifiers
     source          1..12124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2580..3027
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3072..3092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3162..3183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3423..3442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3770..3791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3843..3899
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3975..3992
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6814..7402
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6867..6887)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7285..7296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7405..7421)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7406..7422)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7927..8107
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8133..8150)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8139..8363
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8409..8837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8496..8515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8706..8727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8846..8866)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8851..9180
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9031..9166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9093..9112
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9228..9275
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9294..9668
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9798..9919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9835..9854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9889..9908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9968..9984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9968..9984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9981..10003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9992..10008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10016..10022,10023..10040,10041..10046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10061..10082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10199..10216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10370..10958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10450..10469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11129..11920,11921..11989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11752..11771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11990..12094)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta atcgcatgct agagagggcc ctgcgtatga
     2581 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     2641 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     2701 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     2761 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     2821 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     2881 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     2941 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     3001 ggagtcgtgt cgtgcctgag agcgcagtcg gatccgccac catggctcca aaaaagaaga
     3061 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatgg
     3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3301 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     3841 gagctactaa cttcagcctg ctgaagcaag caggagatgt tgaagaaaac cccgggcctg
     3901 ctagcatgag caccatgcac ctgctgacat tcgccctgct tttttcctgc tccttcgccc
     3961 gcgccgccac taggcatcat caccatcacc acgctagttg cgaccccaag atcgtcaaca
     4021 tcggcgcggt gctgagcacg cgcaagcatg aacagatgtt ccgcgaggca gtaaaccagg
     4081 ccaataagcg acacggctct tggaagatac agctcaacgc cacttctgtc acccacaagc
     4141 ccaacgccat acagatggcc ctgtcagtgt gtgaggacct catctctagc caggtctacg
     4201 ctatcctagt tagccacccg cctactccca acgaccactt cactcccacc cctgtctcct
     4261 acacagctgg cttctacaga atccctgtcc tgggactgac tacccgaatg tccatctact
     4321 ctgacaagag tatccacctg agtttccttc gcacggtgcc gccctactcc caccagtcca
     4381 gcgtctggtt tgagatgatg cgagtctaca actggaacca catcatcctg ctggtcagcg
     4441 acgaccacga gggacgggca gcgcagaagc gcttggagac gttgctggag gaacgggagt
     4501 ccaaggcaga gaaggtgctg cagtttgacc caggaaccaa gaatgtgacg gctctgctga
     4561 tggaggcccg ggaactggag gcccgggtca tcatcctttc tgcaagcgag gacgacgctg
     4621 ccacagtgta ccgcgcagcc gcaatgctga acatgacggg ctctgggtac gtgtggctgg
     4681 tcggggaacg cgagatctct gggaacgccc tgcgctacgc tcctgatggc atcatcggac
     4741 ttcagctcat caatggcaag aatgagtcag cccacatcag tgacgccgtg ggcgtggtgg
     4801 cacaggcagt tcacgaactc ctagagaagg agaatatcac tgacccaccg cggggttgcg
     4861 tgggcaacac caacatctgg aagacaggac cattgttcaa gagggtgctg atgtcttcta
     4921 agtatgcgga cggagtgact ggccgtgtgg aattcaatga ggatggggac cggaagtttg
     4981 ccaactatag tatcatgaac ctgcagaacc gcaagctggt gcaagtgggc atctacaatg
     5041 gtacccatgt catcccaaat gacaggaaga tcatctggcc aggaggagag acagagaaac
     5101 ctcgaggata ccagatgtcc accagactaa agatagtgac aatccaccaa gagcccttcg
     5161 tgtacgtcaa gcccacaatg agtgatggga catgcaaaga ggagttcaca gtcaatggtg
     5221 acccagtgaa gaaggtgatc tgtacggggc ctaatgacac gtccccaggc agcccacgcc
     5281 acacagtgcc ccagtgctgc tatggcttct gcatagacct gctcatcaag ctggcgcgga
     5341 ccatgaattt tacctatgag gtgcacctgg tggcagatgg caagtttggc acacaggagc
     5401 gggtaaacaa cagcaacaaa aaggagtgga acggaatgat gggcgagcta ctcagtggcc
     5461 aagcggacat gattgtggca ccactgacca tcaacaatga gcgtgcgcag tacatagagt
     5521 tctccaagcc cttcaagtac cagggcctga ccattttggt caagaaggag attcccagga
     5581 gcacactgga ctcatttatg cagccttttc agagcacact gtggttgcta gtaggactgt
     5641 cagttcatgt ggtggctgtg atgctgtacc tgctggaccg cttcagtccc tttggccgat
     5701 tcaaggtgaa cagtgaggag gaggaggaag atgcactgac cctgtcctct gccatgtggt
     5761 tttcctgggg cgtcctgctc aactccggca ttggggaagg tgccccccgg agtttctctg
     5821 cacgtatcct aggcatggtg tgggctggtt tcgccatgat catagtggct tcctacactg
     5881 ccaacttggc agctttcctg gtgctggatc ggcctgagga gcgcatcacg ggcatcaatg
     5941 accccaggct cagaaacccc tcagacaagt tcatctacgc aactgtaaag cagagctccg
     6001 tggacatcta cttccggagg caggtggagt tgagtaccat gtaccggcac atggaaaaac
     6061 acaattacga gagcgcagct gaggccatcc aggctgtgcg ggacaacaag ctgcacgcct
     6121 ttatctggga ctcggccgtg ctggagtttg aggcttcaca gaagtgcgat ctggtgacca
     6181 cgggtgagct gttcttccgc tcaggctttg gcatcggcat gcgcaaggac agcccctgga
     6241 agcagaacgt ttccctgtcc atactcaagt cccatgagaa tggcttcatg gaagatctgg
     6301 ataagacatg ggttcggtat caggaatgcg actcccgcag caatgctcct gcaaccctca
     6361 cttttgagaa catggcaggg gtcttcatgc tggtggctgg aggcatcgta gctgggattt
     6421 tcctcatttt cattgagatc gcctacaagc gacacaagga tgcccgtagg aagcagatgc
     6481 agctggcttt tgcagccgtg aacgtgtgga ggaagaacct gcaggataga aagagtggta
     6541 gagcagagcc cgaccctaaa aagaaagcca catttagggc tatcacctcc accctggcct
     6601 ccagcttcaa gagacgtagg tcctccaaag acacgagcac cgggggtgga cgcggcgctt
     6661 tgcaaaacca aaaagacaca gtgctgccgc gacgcgctat tgagagggag gagggccagc
     6721 tgcagctgtg ttcccgtcat agggagagct gaggcgcgcc ttcgaattaa ttaaggccgc
     6781 gactctagag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     6841 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6901 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6961 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7021 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7081 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7141 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7201 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7261 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7321 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7381 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     7441 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7501 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7561 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7621 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7681 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     7741 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     7801 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     7861 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     7921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8101 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     8161 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     8221 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     8281 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     8341 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     8401 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8461 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8521 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8581 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8641 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8701 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8761 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8821 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     8881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     8941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9001 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     9121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9181 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     9241 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     9301 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     9361 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     9421 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     9481 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     9541 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     9601 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     9661 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     9721 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     9781 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9901 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     9961 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10021 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10081 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10141 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10321 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10381 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10441 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10501 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10561 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10621 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10681 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10741 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10801 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10861 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12121 acac
//
