LOCUS       sequence-330610-       15781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins with crRNA targeting 
            AvPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 15781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS1: pHelper(AvCAST)_AvPSP1
FEATURES             Location/Qualifiers
     source          1..15781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3977..4024
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4173..4191
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4192..4216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9996..10014
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10015..10039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12028..12075
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12282..13073)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13074..13165)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(14226..15308)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15270..15289
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15309..15386)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        15432..15450
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    15451..15475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        15736..15754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    15755..15779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcttagtat attagttaag tataagaagg agatatacat atgattattg atagtaactt
       61 agttacgcag cttccctcag aatcatcttc aaagccaaga gagcttttaa tgtctcagtt
      121 tccatatatg gatttaactt ttagccttac gggtgagaca ttacctttcg accacggcta
      181 tgaattattt tctgcgatcg cccactttga acccaaacta catacattag acacgttggg
      241 aattcagacc attgcaggta ttcccaagga tggtgtaatt aatctgaccc aaaattctag
      301 gcttcgtatt cggataccag taaatcaggt acgtttggtt tatccattgg cgggtaagtc
      361 actgagaatt ggcaagcata caattcgttt gggtattcct gacatctgtt tgcttcaacc
      421 tgcggagaaa ctgcgatcgc gcattgtggt aataagaggc tatgaagaac ctgaaagttt
      481 tttagtcgta gcgcagcgtc agctagaaca actgggtatc caagcaattg ctagcattcc
      541 caccaaagcc aatggcaaac caatacgtag gactatcaaa attaagcgat ttacagtcgt
      601 gggttttgga ttagaagtta taaatttaag cgatgaagac tcactgaggt tgcaaataca
      661 tggtgtagga ggaaagcaaa agatgggttg tggactcttc atgcctatta aggaaaggca
      721 atagcaatta ctcgaaagca gatgatgaag agagcagata accccaaaat tcagcttaat
      781 ctcggcgatc ctagtatgac tttattgcat cgtgctgggg ttgcaggact ttggatgacg
      841 ctcaaaaaac tggaaaaaat ctatccaaca cctgctgaac gtattggtaa tttaacttgg
      901 ttgctaaccc ctcactatat caatcttgat tgggaaggac aggatttcac agttttagac
      961 tggttgctga agcaatcatt tcaaatcagc gatgagggtt taatttccct gacagggtta
     1021 gattctcaaa acatggatat tcaaaatcaa ataattatcc atcaaggtat cacagccact
     1081 tttacacaac ataataaatt ttttaagtct gcgggtcaac aatctaagca actaattatt
     1141 gatggtattc aggttagggt tgattataag aaagctgctt cctatgctca tcaacatttt
     1201 gctaaacatt tatgtgacca gtacggacaa ttgctaaaag agccaattgg tatcagggga
     1261 tggctctatc caggagcagt agttcgccat tatgctttta aagaacaaac agcatttgaa
     1321 gaaaaagcag aatatgctct agcgttgttg ttcgctccta tggcttgtca atattttgtg
     1381 ctgcgatcgc acaccgaacc atcacataca aactatgttt tggttattcc tgaagtaatt
     1441 gatttagaac tttatgcaca ttttttgtgg tgtttgggta acttagatta taaacatttt
     1501 cacgtatcta gcttaggaga tgctggatta aaatttctta attataaaac aacacaaatg
     1561 cctacttcca attttttaaa acgatgtcaa ataatctttt ttagtacaaa agcttggtca
     1621 gaacagcaaa aaactcgtgc agaaatagca atcatagaat taaattcttc agttaattat
     1681 ctttataagc ttagttgtat ttgttttcca gaatgtcaaa ttgttttata taaaaatcaa
     1741 cattatttgc ttccaagttt aataaaagga ataatcgcca ataatctagc aatgggtcgg
     1801 tcttggtggg ctaattggat ttcatttatt aaaaataaaa aatcatttaa acaatttact
     1861 gatgagtata aaggaattaa taagatgatt caggactcag aatgggatat agaagctcaa
     1921 aagctattta ttcaagcttg tcatgaagca ttaagaaaaa tatatgccaa aatttatagc
     1981 agaacaaaag aaggtgaata cgctcaaata gaacgagaaa atacacgcat tttatctcag
     2041 ttaaaacgct gcaccaatac tgacaatttc cgaaaattta ttgcagagtt ttggggaagg
     2101 gcagggcaaa tagcaattct ggaagaacat tggatggaat tacttccatt gactacaggg
     2161 ataggcaatt ggaaagtggc cagggactta acttttattg caatggctag ctacccaaag
     2221 aataaaataa cagaggtaga ggtaaaacca tcacaaatat cggagctaaa tactgaataa
     2281 acttcatatt ttttgatttg attaagaaca aaatattaat tgtttaggta atggtggaat
     2341 gtttcatctg tttggtaata ttttgactag ttatggaacc gccgctaata atcgtggtga
     2401 aaatgaaggt aataccacta cgttacaaaa attaatttgg aaaggtgaag ttcactctac
     2461 tgtttcatct gaggcaattc gttgggcatt gcgatattat tggcaaaatg ctggctatcc
     2521 tgtgaataga cgttgggatg aaaatgctca accagtaccc gatcacatct tgcaagatcc
     2581 taattttgat gatattcgct tcattgatga tgatgtcttg ggatttatgc aagcggaagc
     2641 agcaaaagtt gaagcagcag ttgaatccga agttgaaatc cagaactcaa ctcaagatga
     2701 ggacaaaaaa ctttctcaag gtaaggagaa agaaactgca aaacgtaagc gtaaggaaaa
     2761 acctaaaggg aaaataacta ctagacgagg tgtattagaa gtcactcgtg cagtttcaac
     2821 aattccttat gctggtgatc ttactttcaa tgctatgagt ggaaagaaag gacgaacatc
     2881 tctttattct acagaagttc atgctactcg atatcaatat ggcttcgcat taacacccaa
     2941 tagcctcaaa gataaatctc gcattaatgc tgtcctcgat agcttaattt ctattggaga
     3001 agtggcagga aatcacgctc gttttctcta tgatttttct cctgatagca tcatattacg
     3061 gtggacacac gatttctcac ctcggttact ttactgtttt gaagaagatg aacttagaaa
     3121 tatatcaaca ccggatttag taaggcgggt agaatctgga gatatagacc ctaaagaatt
     3181 atggatagga ggggcaattt ctagcactct ggaagattta ggggcaaatg tgtttcccgg
     3241 agtgaagaca acagttgaag cattaaaaca ggtgattatt gaagatttgc agttgtcgtt
     3301 aaggagttca tgatatgacg acaattgcct taaaagtaga agttcccata gcttgttttc
     3361 ggcagtctcg tgctagggaa tatggcgaaa cttaccctgt accaccacca tcgacagtat
     3421 atggaatgtt actatctctt gttggagaag tggatagata caaacactgc ggtgtgaagc
     3481 tggcgatcgc acttctatct gaaccagaaa agtcaactgt tattcgcacc tttcaccgat
     3541 ttaagacgaa gaatatccat gactctaaaa ataataaacc tgattatcag gagttattaa
     3601 ctaatataga atttatagtt tgggtggatg ctggagtaga taaagctaaa ccaaatttag
     3661 tggaacgttt agcagaggca ttgactaacc cggcttctat taataggttt ggtggattat
     3721 gtttaggtga aagtcgggat ttagtgaatg atgttaccct tttacctaaa aattactatg
     3781 cagaatcaat gcgatggtta attcgagatg aatatgggtt actaacttta ccttactggg
     3841 tagaccatgt aggttcgcgg ggaacgcgat ggctacgtta cgagattcag aaatgtccag
     3901 tctttcaacc acctgaatta tcttggactt cagttcaaag taattagcct aggctgctgc
     3961 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     4021 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     4081 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg gatctcgacg
     4141 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag gggaattgtg
     4201 agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg agatatacca
     4261 tggttagggg tagaagggaa tggactcaag ctaaatatga gcgatatata aaagaaggtc
     4321 gtggccgagg aagtggtaag cactataaac cttggcttac aatccaagat gttccctcta
     4381 aaggacgttc ttctagaact cctggttgga aaacaaatcg agtacatcat ttcttctcag
     4441 accatgaaaa acggctattt tatttgtttg agtggtcaga tatagtcaca gatataagag
     4501 agcaatttcc tttagacaat ctcgatctag ccatgagtat tgctacagac ataggtataa
     4561 agtaccctgt agatacacaa agtggcactc ctcacgtttt aacaagcgac tttatgctta
     4621 cagtcaaccg cgatgggaag cagatgcaga ttgcccggac tgttaagccg tcaacagaac
     4681 tggagaaaaa gcaagttaat gagaaattcg agatagaaag acaatattac ttagcaaagg
     4741 gtattgactg gggaattatc acagaaaaag aaattcccag gcttttagct gaaaatgttg
     4801 agtgggttca ttctgcttat aggctagaag caaattctga aatggacact gcacaactga
     4861 gtgaattgtc aacaatttta aaatctaagc ttcaggaaga agatacaact ataaatagag
     4921 taactactgc tttagacaaa gaaatgaata taaattccgg tacatcactt tatgtattta
     4981 aacatttaat tgctaggaaa gaaatccttg tggatctagt agcaataaaa ttatctgctt
     5041 gtccgtcaac taaagttatt gaaaaaataa tattttagac ccacatgata ggtaaataat
     5101 atggttaaag atttatttgt caatgattta atagagtgga ttgtatacca aaacactcat
     5161 atagtcgaaa gaattatttg gatagatgaa ggttacatta ttgcatttgt tatagatatt
     5221 aatgctaaga aaggtctacc tgaaccaagg aaaatatcag atattttaga agctataact
     5281 gaaggtattg cttttaagca gcaacaagat ccttgggcaa gaattattcg ggatgaaaat
     5341 ttgacagata aggaaaagga atacagagat aaagcttggg agataatttc atcattaatt
     5401 atagaagaac catcaatata ttatagaaat tttagaggat cgatggttaa agaagcggtt
     5461 gaaaagtaca acgaagaaag ggataaagat aaattagtag aaaaaaccgt ctatggttat
     5521 ttgagaagat tttggcagag aggaaaaact ataaatgctc tcatacctga ttttatcaat
     5581 tcatcaggta agggcaagat tcgaggacaa tcgggcaaga agagaggaag accaagaaaa
     5641 tatgcacatg acccagatat tggtgaaggt gtaaatgtta cagaagaaga tagaaagatt
     5701 tttagagtat ctataagcaa attttacaat aatccaaaag aaaactactt aacaacagcc
     5761 tatgaattaa tggtgaaaga atattacaag gaaggaatta gatatgacga gaatggtgtt
     5821 caaaaatcta tattaacccc aactgataaa atacctacaa ttactcaatt caaatactgg
     5881 tatcaacttg aacaggatga tgttaccaaa actttaacat ctcgaaaagg agccaagcga
     5941 gtagctttag agaatagagc aattttggga aactctaaga tggaaactat tggccctggt
     6001 tctagatatc aaattgatgc cacagttgct gatgtatatt tagtttcaaa atataaccgt
     6061 gactggataa ttggtagacc agttatctat gtaattattg atgtttttag tagaatgatt
     6121 acaggtgttt atgtcggttt agaaggccca tcttggatag gagcaatgat ggcactagcc
     6181 aatgtagcta cagataaaac cctgttttgc caggaatata acattaatat tactgaaaat
     6241 gaatggcctt gcaatcaaat gccagatgca attttaggag ataggggaga attagcaggt
     6301 atgacagtag aaacattaat ccctaatctt tatgttcgta ttgaaaatgc agcttcctat
     6361 agagcagatt ggaaagggtt agtagaaaga cactttcgta taattcatga atatgtaaaa
     6421 ccttttttac ctggttatgt ggatacggat tttaggcaaa gaggtgcaag ggattatagg
     6481 cttgatggca aacttgatat agatcaattt actgagatta ttattcgttt aattgttcac
     6541 cataacaata aacctcttac tgaatatgaa agagatgaag caatgattgc tgatgatacc
     6601 cctcctatac ctagagagtt gtggaagtgg ggtatcgcca atcgttctgg cagactcaga
     6661 actttttctg aagatattgt caaattgaac ttgatgccta ctgagaaagc aactattact
     6721 gctaaaggca tcaaaatgag aggtaaggag atgtactata cctgtgatag tgctaaaaat
     6781 gagcaatggt ttgaaaaagc tagaagtaat ctcctttcta agtcagaaaa atcattgatt
     6841 gtatcttatg acatcagaaa gccaaacttc atttatcttc catctagtga tggtagagat
     6901 tttgaaaaat gttttcttat tgacttagaa gggcggtatg ctaataaaaa ctttcatgat
     6961 attgaatatc tgcttgctta tgaagaattg caaagacaga aaagccaagg taaacagcta
     7021 caagaaaagg tagatttaat agctgatatt gaaagcatag tagatagagc agaaaaaatg
     7081 actgatgctt ctcaatatca taatttaagt aagagcaaga aagtttctgg aataagagat
     7141 aatagggctt ttgaaaaggc taaacgacga gagaatgaag gatttgaatt agcaattaca
     7201 gataatcaga gcagttttca ggttgcagag agtacagcag aaacctccga acagccagaa
     7261 tcactacagc caaaccatct gaatcttctt aagaaaaagc gacaggagcg aaaacgtgga
     7321 cagaattgag agtgaactgg ttaaaattcc taatggagaa ctagcagtta ttgccagata
     7381 tatagatcaa aaactgccag aatacaacac taatccatta attcaggcat tacctcctat
     7441 cctatcagca gcagaattta tagataaagt agccagagaa ccaaattttg atgaacaaga
     7501 gagagagcta gaaccacatt atagatttca ttgtattgag aggttatcta gatattttga
     7561 ccctcaaaat aaaactcttg aattacaaaa agttatttgt acgcttatca tgacaggcta
     7621 tctgtcaagg aaccttttaa aacctgaata tgcaaatcgc tctaggcaaa tttataatgc
     7681 aataggtaat ggaggaggta aaaatttaga aaactatgtt aatcttccta catctgcgtc
     7741 cggcttaact ctaattggcc catcaggtat tggaaagact acaaatttca aaaatattct
     7801 tgagctttat ccccaaataa tttttcatcc tgattatagt gtttaccaaa tagtttggct
     7861 gcgggtagat tgccctaaaa ctttaaaggg gttatgtata gatacttttg caggtatcga
     7921 taaattgtta ggaactaatt tattaaagaa atttaaccca gcacgcacat ctgaagatta
     7981 tctattggct caacttgcac aaacagcaca cactcatcat ctaggagttt tggtgattga
     8041 tgaaatacaa aatttagcaa ctgcaaaaag aaatagagac gaaatattaa attttttagt
     8101 aaagctcgat aatactattg gaattccagt tattcgtgta ggtacgaatg aagccattag
     8161 ttttcttcaa ggtaatctta gaaatgctag aagaaatact ggagaaggaa gtagaatgtg
     8221 ggatagaatg gtaaataatg atgaatggtg ttacttttta gaaggaatat gggaatacca
     8281 atggacaaaa acacctgttc atttctcgga tgaaataaaa gaagtttttt atgatgaaag
     8341 ccagggaatt attgatattg ctataaaact ttacaaaatg gttcaatgga gagcgatttc
     8401 aataggtgac caagaaatta ttacggttga tctgattcgt caagtagcta aagaaggatt
     8461 gtatttagta aagccaatgc tagatgcaat taggttaggg gacagagagt ggatgattaa
     8521 atataaggat attgctcctc tcgatattac ggaatatcac aatagatgtg tatctgagat
     8581 tgatgctaaa gatttgacag agattaagag attagctaaa aagcagcaga atcagcagaa
     8641 agtatctccg aagctcaatt atgtaattag tgagctgttg aaattggaag tagaatctgg
     8701 taaagcaaag gaatgtgcag aacaaattgt ttatagtcat gaagatccag acattccaat
     8761 tccatcttta gttaataagg catatgctct tgctttgcaa ggatgtcaag taaatgagag
     8821 tcttgctaaa acaagtacca ggaaagctaa attaaagaca aagtaccaag agaatgatat
     8881 tcggcgaatt gtcgagaatg ccaagaaaga caaaaaatct gcatacgagg atttgaaatt
     8941 agcaggtata atcaaaaatc cagttgaaga ttttatcaat atttagcttc taaatgctta
     9001 gttttttccc aactctgtat cccgatgaac ttttatatag cactttagct agataccata
     9061 ttcggagtgg aaataaaagt ttcaagcaaa ctgacttaga gctattcggt tacagttccc
     9121 aacaaatatg caaaattacc ttaactaata atttaaatta tttagtgaaa aacttacctt
     9181 tacgctccca gcaaaaagtt gagaatttac tacaaaatca tactttatat tccttttatg
     9241 caactttatt aatgccgcag gaggcttggc tgctgaaaaa ctcaatgagt aataaattca
     9301 gtgggtctat actagatttt gctaaagtgg ctaataattc tagtgatgat tcaaaaaaat
     9361 ttttaaaatt ttgccctgaa tgtttagaga aagataccca aaagtatggt gaaccttact
     9421 ggcatagaat acatcaagtc cctggcattt tagtttgtcc aattcatcgt gttgttttac
     9481 acaatagctg tatagaagtt gaatcgaaag gcatacatta tcatgctgcc agcatagata
     9541 attgtttatt agcaaataat gtgaaaaaat tgacaaataa aactgtagaa acactttcaa
     9601 tactggcaca tgatattaat tggctaattc atagcagttt tgcctttcaa ggtttggctt
     9661 ggcttcgcgc tcagtaccag cgttacttat tggataaagg actgctaaaa acctttgctg
     9721 gtggcaagtt taagtttaat gattgtggtt ttgcccagct tatatttgag ttttatggtc
     9781 aagaatcctt agaagccctc aatccaaagc tgacccaaaa tcaaggtaaa tatttttctc
     9841 actgcttgtt tggatgcgac ttaaacccag caattgaccg cataactcat atcttaatga
     9901 taaaattttt agcaaattct atagaagagt tctttcagtc ataacttaag tcgaacagaa
     9961 agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta taggggaatt
    10021 gtgagcggat aacaattccc catcttagta tattagttaa gtataagaag gagatataca
    10081 tggtgagttt tttccctcaa ccttatccag atgaaatttt atatagtgtt attgctcgtt
    10141 atcacattag aagtggcaat acgagtccaa aaattacatt acaagattta tttaagtcac
    10201 agactactgt ggcgacagta gatttaccat cgaaccttag taatttaatt aaaaacctac
    10261 aatttatctc aaattaccaa gttgaagagt tgatatataa acatactctc taccctttat
    10321 atagcccttt tttgccagca aacaggtcta gtcaggtact ggaatctatg aaaggagatt
    10381 atgggggaga tattcataca agagccggaa ttatggcaag ctcaattact atgccgaaat
    10441 atttgcggtt ttgtccaact tgctgggaag aagacttaaa aaattatggc gaaccttact
    10501 ggcatcgcat ccatcaaata ccagggattt tagtttgtcc atttcatggt gaagttttgc
    10561 aagatagcct agctccttta catggtttta ataagcatga atattatgca gctagtacag
    10621 acaactgttc tattactaaa gaacccagta ttcttagccc tgacacactg aaaaaattac
    10681 taatattagc caaagatttt tggtggttac tcaatagtga aataccttgc caagagccac
    10741 agtggtttcg caaacagtac acaaatctga tgattgagca gggttttgca actccaactg
    10801 gtcgaattta ccagaacaaa ctactagata attttttgtt cttttatggt caagaaatgc
    10861 tcagtgctgt tgattcaatg gtaaatcata atgattcaaa caactgggtt actagcatcg
    10921 tcaggaaata taggaaatct ttccatcctc ttagacatct gctaatgatg cgttttttga
    10981 aaacatcagt agaggaattt tttcaaaaag atactgaata caagcctttt ggcgaacccc
    11041 cctggttgtg tctcaatgct gccgcagaac attatctaca accagtgatt accaatttag
    11101 tcattacttt atgctgcgat acaaaaaagc ctgtcggtac tttttcttgt gcttgcggaa
    11161 tggtttattg ccggactggg cctgatgaaa cggatgaaga caaagttcgc attggtaaag
    11221 tcaaggcatt cggtcaaata tgggagcaaa agctgaaaga attggtggaa gttgaaaagt
    11281 tgggtttgcg ggagacagcc agaagattaa atgttgatgc cagaactatc aaacgttatg
    11341 tatctcgtct aaaattgaca acttattggc agactagaac agaaaatgac tctgtaggct
    11401 tacagggagt cccagaaata gatcccaatt ttgcaactga actcaaatta aaacacaggc
    11461 aaaattggat ggctttacaa gtacagaacc ctgaagcttc aaagacaact ctgagaaaac
    11521 tagtaccagc cacctatatt tggctataca ggaatgacag agaatggctc aatcaaaact
    11581 cacccacttt acaagttgcc gttccttccg ttgtgagagt tgattggcat gagcgagatc
    11641 agcaaattct cgctcaggtt caagatgcag tgcgatcgct acttaaggct gatataccag
    11701 agaaaatttc tattagtagg gtagggaaaa ctatcggctt acttgcatta ctcgaaaaac
    11761 atctcgacca gatgccttta acaaaggctt atttggaatc agtcgttgaa actgtagaag
    11821 aatttcagat gcgccggatc aagtgggcaa tcaagctgct tgatgaatgt ggagaagaaa
    11881 ttatgcgctg gaaggtagtc agagttgctg ctttgcgaga ggattgctcc caaagagtca
    11941 gtgcatttct ggaaagtgaa ctggagaaag catacaagaa agagaggtga ttaattaacc
    12001 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
    12061 ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg
    12121 gtagtccggt caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct
    12181 gccctgaacc gacgacaagc tgacgaccgc tgccctgaac cgacgaccgg gtcatcgtgg
    12241 ccggatcttg cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg
    12301 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
    12361 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
    12421 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
    12481 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
    12541 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
    12601 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
    12661 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
    12721 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
    12781 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
    12841 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
    12901 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
    12961 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
    13021 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc
    13081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13141 gaatgtattt agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg
    13201 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
    13261 gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc
    13321 gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga
    13381 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
    13441 tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
    13501 ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta
    13561 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
    13621 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg
    13681 taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta
    13741 aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg
    13801 gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc
    13861 ttcgatcaaa ccacctcccc aggtggtttt ttcgaaggtt aagtcagttg gggaactgct
    13921 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
    13981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt
    14041 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
    14101 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
    14161 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
    14221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    14281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
    14341 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
    14401 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
    14461 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
    14521 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
    14581 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
    14641 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
    14701 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
    14761 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
    14821 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
    14881 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
    14941 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
    15001 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
    15061 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
    15121 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
    15181 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
    15241 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
    15301 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
    15361 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
    15421 cattaggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctgt
    15481 agaaataatt ttgtttaact ttaataagga gatataccat ggtgctttaa cattagatgt
    15541 cgttaggcgt tgagcagaga agtcatttaa taaggccact gttaaacgta gatgtgcttt
    15601 aacattagat gtcgttaggc gttgagcaga tccgaattcg agctcggcgc gcctgcaggt
    15661 cgacaagctt gcggccgcat aatgcttaag tcgaacagaa agtaatcgta ttgtacacgg
    15721 ccgcataatc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
    15781 c
//
