LOCUS       sequence-330611-       12667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SNAP tagged NR1 in neuronal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Li S, Raychaudhuri S, Lee SA, Brockmann MM, Wang J, Kusick G, Prater
            C, Syed S, Falahati H, Ramos R, Bartol TM, Hosy E, Watanabe S
  TITLE     Asynchronous release sites align with NMDA receptors in mouse 
            hippocampal synapses.
  JOURNAL   Nat Commun. 2021 Jan 29;12(1):677. doi: 10.1038/s41467-021-21004-x.
  PUBMED    33514725
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-29"; volume: "12(1):677. doi"; pages: " 
            10.1038/s41467-021-21004-x"
COMMENT     Sequence Label: pSynapsin_NLS_GFP_P2A_SNAP_NR1
FEATURES             Location/Qualifiers
     source          1..12667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2580..3027
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3072..3092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3096..3116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3117..3119,3120..3122,3123..3833)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3162..3183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3423..3442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3770..3791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3843..3899
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7357..7945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7410..7430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7828..7839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7948..7964)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7949..7965)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8470..8650
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8676..8693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8682..8906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8952..9380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9039..9058)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9249..9270
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9389..9409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9394..9723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9574..9709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9636..9655
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9771..9818
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9837..10211
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10341..10462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10378..10397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10432..10451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10511..10527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10511..10527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10524..10546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10535..10551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10559..10565,10566..10583,10584..10589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10604..10625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10742..10759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10913..11501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10993..11012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11672..12463,12464..12532))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12295..12314
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12533..12637)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta atcgcatgct agagagggcc ctgcgtatga
     2581 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     2641 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     2701 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     2761 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     2821 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     2881 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     2941 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     3001 ggagtcgtgt cgtgcctgag agcgcagtcg gatccgccac catggctcca aaaaagaaga
     3061 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatgg
     3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3301 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3721 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatccg
     3841 gagctactaa cttcagcctg ctgaagcaag caggagatgt tgaagaaaac cccgggcctg
     3901 ctagcatgag caccatgcac ctgctgacat tcgccctgct tttttcctgc tccttcgccc
     3961 gcgccgccac taggatgggc ctgggctcca tggacaagga ttgtgaaatg aaacgcacca
     4021 cactggacag ccctttgggg aagctggagc tgtctggttg tgagcagggt ctgcacgaaa
     4081 taatttttct gggcaagggg acgtctgcag ctgatgccgt ggaggtccca gcccccgctg
     4141 cggttctcgg aggtccggag cccctgatgc aggctacagc ctggctgaat gcctatttcc
     4201 accagcccga ggctatcgaa gagttccccg tgccggctct tcaccatccc gttttccagc
     4261 aagagtcgtt caccagacag gtgttatgga agctgctgaa ggttgtgaaa ttcggagaag
     4321 tgatttctta ctctcactta gcagccctgg caggcaaccc cgcagccacg gcagcagtga
     4381 agacggcact gagtggcaat cctgtcccta tcctgatccc gtgccacaga gtggtccagg
     4441 gggatttgga tgtgggcggt tacgagggtg gactggccgt gaaggaatgg cttctggccc
     4501 atgaaggcca ccggttgggg aagccaggct tgggagctag ttgcgacccc aagatcgtca
     4561 acatcggcgc ggtgctgagc acgcgcaagc atgaacagat gttccgcgag gcagtaaacc
     4621 aggccaataa gcgacacggc tcttggaaga tacagctcaa cgccacttct gtcacccaca
     4681 agcccaacgc catacagatg gccctgtcag tgtgtgagga cctcatctct agccaggtct
     4741 acgctatcct agttagccac ccgcctactc ccaacgacca cttcactccc acccctgtct
     4801 cctacacagc tggcttctac agaatccctg tcctgggact gactacccga atgtccatct
     4861 actctgacaa gagtatccac ctgagtttcc ttcgcacggt gccgccctac tcccaccagt
     4921 ccagcgtctg gtttgagatg atgcgagtct acaactggaa ccacatcatc ctgctggtca
     4981 gcgacgacca cgagggacgg gcagcgcaga agcgcttgga gacgttgctg gaggaacggg
     5041 agtccaaggc agagaaggtg ctgcagtttg acccaggaac caagaatgtg acggctctgc
     5101 tgatggaggc ccgggaactg gaggcccggg tcatcatcct ttctgcaagc gaggacgacg
     5161 ctgccacagt gtaccgcgca gccgcaatgc tgaacatgac gggctctggg tacgtgtggc
     5221 tggtcgggga acgcgagatc tctgggaacg ccctgcgcta cgctcctgat ggcatcatcg
     5281 gacttcagct catcaatggc aagaatgagt cagcccacat cagtgacgcc gtgggcgtgg
     5341 tggcacaggc agttcacgaa ctcctagaga aggagaatat cactgaccca ccgcggggtt
     5401 gcgtgggcaa caccaacatc tggaagacag gaccattgtt caagagggtg ctgatgtctt
     5461 ctaagtatgc ggacggagtg actggccgtg tggaattcaa tgaggatggg gaccggaagt
     5521 ttgccaacta tagtatcatg aacctgcaga accgcaagct ggtgcaagtg ggcatctaca
     5581 atggtaccca tgtcatccca aatgacagga agatcatctg gccaggagga gagacagaga
     5641 aacctcgagg ataccagatg tccaccagac taaagatagt gacaatccac caagagccct
     5701 tcgtgtacgt caagcccaca atgagtgatg ggacatgcaa agaggagttc acagtcaatg
     5761 gtgacccagt gaagaaggtg atctgtacgg ggcctaatga cacgtcccca ggcagcccac
     5821 gccacacagt gccccagtgc tgctatggct tctgcataga cctgctcatc aagctggcgc
     5881 ggaccatgaa ttttacctat gaggtgcacc tggtggcaga tggcaagttt ggcacacagg
     5941 agcgggtaaa caacagcaac aaaaaggagt ggaacggaat gatgggcgag ctactcagtg
     6001 gccaagcgga catgattgtg gcaccactga ccatcaacaa tgagcgtgcg cagtacatag
     6061 agttctccaa gcccttcaag taccagggcc tgaccatttt ggtcaagaag gagattccca
     6121 ggagcacact ggactcattt atgcagcctt ttcagagcac actgtggttg ctagtaggac
     6181 tgtcagttca tgtggtggct gtgatgctgt acctgctgga ccgcttcagt ccctttggcc
     6241 gattcaaggt gaacagtgag gaggaggagg aagatgcact gaccctgtcc tctgccatgt
     6301 ggttttcctg gggcgtcctg ctcaactccg gcattgggga aggtgccccc cggagtttct
     6361 ctgcacgtat cctaggcatg gtgtgggctg gtttcgccat gatcatagtg gcttcctaca
     6421 ctgccaactt ggcagctttc ctggtgctgg atcggcctga ggagcgcatc acgggcatca
     6481 atgaccccag gctcagaaac ccctcagaca agttcatcta cgcaactgta aagcagagct
     6541 ccgtggacat ctacttccgg aggcaggtgg agttgagtac catgtaccgg cacatggaaa
     6601 aacacaatta cgagagcgca gctgaggcca tccaggctgt gcgggacaac aagctgcacg
     6661 cctttatctg ggactcggcc gtgctggagt ttgaggcttc acagaagtgc gatctggtga
     6721 ccacgggtga gctgttcttc cgctcaggct ttggcatcgg catgcgcaag gacagcccct
     6781 ggaagcagaa cgtttccctg tccatactca agtcccatga gaatggcttc atggaagatc
     6841 tggataagac atgggttcgg tatcaggaat gcgactcccg cagcaatgct cctgcaaccc
     6901 tcacttttga gaacatggca ggggtcttca tgctggtggc tggaggcatc gtagctggga
     6961 ttttcctcat tttcattgag atcgcctaca agcgacacaa ggatgcccgt aggaagcaga
     7021 tgcagctggc ttttgcagcc gtgaacgtgt ggaggaagaa cctgcaggat agaaagagtg
     7081 gtagagcaga gcccgaccct aaaaagaaag ccacatttag ggctatcacc tccaccctgg
     7141 cctccagctt caagagacgt aggtcctcca aagacacgag caccgggggt ggacgcggcg
     7201 ctttgcaaaa ccaaaaagac acagtgctgc cgcgacgcgc tattgagagg gaggagggcc
     7261 agctgcagct gtgttcccgt catagggaga gctgaggcgc gccttcgaat taattaaggc
     7321 cgcgactcta gagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     7381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7741 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7921 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat
     7981 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8041 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8101 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8161 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8221 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     8281 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     8341 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     8401 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     8461 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8521 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8581 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8641 atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     8701 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8761 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8821 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8881 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8941 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9001 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9061 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9121 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9181 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9241 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9301 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9361 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9421 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9481 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9541 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9601 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9661 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9721 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     9781 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     9841 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     9901 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     9961 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    10021 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    10081 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    10141 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    10201 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    10261 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    10321 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    10381 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10441 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    10501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    10561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    10621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    10681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    10741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    10801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    10861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    11281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    11341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    11401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    11461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    11521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    11581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    12301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    12361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    12421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    12481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    12541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12661 ctgacac
//
