LOCUS       sequence-33062-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP214
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttatgcc
       61 atcctcatga aaactgtgta acataataac cgaagtgtcg aaaaggtggc accttgtcca
      121 attgaacacg ctcgatgaaa aaaataagat atatataagg ttaagtaaag cgtctgttag
      181 aaaggaagtt tttccttttt cttgctctct tgtcttttca tctactattt ccttcgtgta
      241 atacagggtc gtcagataca tagatacaat tctattaccc ccatccatac aatgccatct
      301 catttcgata ctgttcaact acacgccggc caagagaacc ctggtgacaa tgctcacaga
      361 tccagagctg taccaattta cgccaccact tcttatgttt tcgaaaactc taagcatggt
      421 tcgcaattgt ttggtctaga agttccaggt tacgtctatt cccgtttcca aaacccaacc
      481 agtaatgttt tggaagaaag aattgctgct ttagaaggtg gtgctgctgc tttggctgtt
      541 tcctccggtc aagccgctca aacccttgcc atccaaggtt tggcacacac tggtgacaac
      601 atcgtttcca cttcttactt atacggtggt acttataacc agttcaaaat ctcgttcaaa
      661 agatttggta tcgaggctag atttgttgaa ggtgacaatc cagaagaatt cgaaaaggtc
      721 tttgatgaaa gaaccaaggc tgtttatttg gaaaccattg gtaatccaaa gtacaatgtt
      781 ccggattttg aaaaaa
//
