LOCUS       sequence-33063-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP216
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..58)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        59..340
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ggtcgactct agaggatccc cgggataact tcgtatagca tacattatac gaagttataa
       61 cgacattact atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg
      121 tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc
      181 ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa
      241 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
      301 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
      361 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
      421 tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
      481 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
      541 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
      601 tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga
      661 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
      721 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
      781 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
      841 agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgcc
//
