LOCUS       sequence-330656-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd 
            generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Newman R, Tolar P
  TITLE     Genome-wide screens identify calcium signaling as a key regulator of
            IgE+ plasma cell differentiation and survival
  JOURNAL   biorXiv 2021.03.02.433398
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "biorXiv 
            2021.03.02.433398"
COMMENT     Sequence Label: LentiGuide Cherry
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(133..151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     401..420
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
       61 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      121 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      181 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      241 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      301 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
      361 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      421 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      481 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
      541 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
      601 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      661 gccactccac cggcggcatg gacgagctgt acaagtaa
//
