LOCUS       sequence-330658-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_S.FLAG.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1016..1060
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1079..1102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1124..1147
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    1910..2498
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1963..1983)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2381..2392)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2573..2806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2878..2999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2915..2934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2969..2988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3039..3174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3194..3213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3195..3213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3223..3240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3223..3239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3232..3254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3381..3836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3468..3487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3678..3699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3862..3966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3967..4035,4036..4827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4185..4204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4998..5586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5487..5506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5740..5757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5874..5895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5910..5915,5916..5933,5934..5940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5948..5964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5953..5975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5972..5988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5972..5988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6007..6027
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6009..6027
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6055..6281
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6282..6462
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6509..6634
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7127..7360
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7545..7589
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7887..8135
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7887..7907
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8058..8077
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8154..8229
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8277..8394
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8534..8847,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8765..8847,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcaagga
     1021 gaccgccgcc gccaagttcg agaggcagca catggacagc ggaggaggcg ggggagggga
     1081 ctacaaagac gatgacgaca agggaggagg cgggggaggg aactggtccc acccccaatt
     1141 cgagaaaccc acaggcctgt acacacacag cggtgagtgc tgcaaagcct gcaacctggg
     1201 cgagggtgtg gcccagcctt gtggagccaa ccagaccgtg tgtgagccct gcctggacag
     1261 cgtgacgttc tccgacgtgg tgagcgcgac cgagccgtgc aagccgtgca ccgagtgcgt
     1321 ggggctccag agcatgtcgg cgccgtgcgt ggaggccgac gacgccgtgt gccgctgcgc
     1381 ctacggctac taccaggatg agacgactgg gcgctgcgag gcgtgccgcg tgtgcgaggc
     1441 gggctcgggc ctcgtgttct cctgccagga caagcagaac accgtgtgcg aggagtgccc
     1501 cgacggcacg tattccgacg aggccaacca cgtggacccg tgcctgccct gcaccgtgtg
     1561 cgaggacacc gagcgccagc tccgcgagtg cacacgctgg gccgacgccg agtgcgagga
     1621 gatccctggc cgttggatta cacggtccac acccccagag ggctcggaca gcacagcccc
     1681 cagcacccag gagcctgagg cacctccaga acaagacctc atagccagca cggtggcagg
     1741 tgtggtgacc acagtgatgg gcagctccca gcccgtggtg acccgaggca ccaccgacaa
     1801 cctcatccct gtctattgct ccatcctggc tgctgtggtt gtgggccttg tggcctacat
     1861 agccttcaag aggtggaaca ggggtatcct ctagagcggc cgcgtcgaca atcaacctct
     1921 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     1981 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2041 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2101 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2161 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2221 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2281 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     2341 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2401 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcatcttc gccttcgccc
     2461 tcagacgagt cggatctccc tttgggccgc ctccccgcct gggtaccttt aagaccaatg
     2521 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     2581 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
     2641 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2701 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2761 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     2821 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     2881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3001 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3061 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3121 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     3181 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     3241 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3301 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3361 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3781 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     3841 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3901 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3961 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4021 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4081 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4141 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4201 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4261 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4321 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4381 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4441 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4501 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4561 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4621 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4681 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4741 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4801 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4861 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4921 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5581 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5641 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5701 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5761 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5821 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5881 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5941 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6001 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     6061 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     6121 aaggagagaa aaagcaccgt gcatggcgat tggtggaagt aaggtggtac gatcgtgcct
     6181 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     6241 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     6301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     6481 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     6541 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     6601 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     6661 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     6721 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     6781 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     6841 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     6901 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     6961 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7021 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7081 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7141 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     7201 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     7261 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     7321 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     7381 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     7441 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     7501 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     7561 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7621 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7681 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7741 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     7801 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     7861 agtatcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     7921 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     7981 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     8041 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     8101 tggctttata tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cctgttttag
     8161 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     8221 agtcggtgct ttttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
     8281 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8341 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     8401 tattacaggg acagcagaga tccactttgg ccgcggctcg aggcgataag ctttgcaaag
     8461 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     8521 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     8581 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     8641 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     8701 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     8761 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     8821 cgtgccttga attacttcca cctggct
//
