LOCUS       sequence-33066-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP222
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttattaa
       61 ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa
      121 ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca
      181 actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa
      241 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt
      301 aaggacctat tgtttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta
      361 ccttttacat ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc
      421 cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc
      481 attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat
      541 catttaattg gtggtgctgc tatcgaagct acaggtgtcc cacttccaga tgaggcgctg
      601 gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt
      661 acaggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac
      721 gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag
      781 ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac
      841 tttggtaag
//
