LOCUS       sequence-330664-        8850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_S.NWS.HA_NGFR
FEATURES             Location/Qualifiers
     source          1..8850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1016..1060
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1082..1105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1124..1150
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1913..2501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1966..1986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2384..2395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2576..2809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2881..3002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2918..2937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2972..2991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3042..3177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3197..3216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3198..3216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3226..3243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3226..3242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3235..3257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3384..3839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3471..3490)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3681..3702
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3865..3969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3970..4038,4039..4830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4188..4207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5001..5589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5490..5509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5743..5760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5877..5898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5913..5918,5919..5936,5937..5943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5951..5967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5956..5978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5975..5991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5975..5991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6010..6030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6012..6030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6058..6284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6285..6465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6512..6637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7130..7363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7548..7592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7890..8138
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7890..7910
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8061..8080
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8157..8232
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8280..8397
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8537..8850,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8768..8850,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcaagga
     1021 gaccgccgcc gccaagttcg agaggcagca catggacagc ggaggaggcg ggggagggaa
     1081 ctggtcccac ccccaattcg agaaaggagg aggcggggga gggtacccat acgatgtgcc
     1141 agattacgct cccacaggcc tgtacacaca cagcggtgag tgctgcaaag cctgcaacct
     1201 gggcgagggt gtggcccagc cttgtggagc caaccagacc gtgtgtgagc cctgcctgga
     1261 cagcgtgacg ttctccgacg tggtgagcgc gaccgagccg tgcaagccgt gcaccgagtg
     1321 cgtggggctc cagagcatgt cggcgccgtg cgtggaggcc gacgacgccg tgtgccgctg
     1381 cgcctacggc tactaccagg atgagacgac tgggcgctgc gaggcgtgcc gcgtgtgcga
     1441 ggcgggctcg ggcctcgtgt tctcctgcca ggacaagcag aacaccgtgt gcgaggagtg
     1501 ccccgacggc acgtattccg acgaggccaa ccacgtggac ccgtgcctgc cctgcaccgt
     1561 gtgcgaggac accgagcgcc agctccgcga gtgcacacgc tgggccgacg ccgagtgcga
     1621 ggagatccct ggccgttgga ttacacggtc cacaccccca gagggctcgg acagcacagc
     1681 ccccagcacc caggagcctg aggcacctcc agaacaagac ctcatagcca gcacggtggc
     1741 aggtgtggtg accacagtga tgggcagctc ccagcccgtg gtgacccgag gcaccaccga
     1801 caacctcatc cctgtctatt gctccatcct ggctgctgtg gttgtgggcc ttgtggccta
     1861 catagccttc aagaggtgga acaggggtat cctctagagc ggccgcgtcg acaatcaacc
     1921 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     1981 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2041 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2101 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2161 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2221 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2281 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     2341 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     2401 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcatc ttcgccttcg
     2461 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgggtacc tttaagacca
     2521 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2581 gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg gtctctctgg
     2641 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2701 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2761 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     2821 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     2881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3001 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3061 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3121 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3181 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3361 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3421 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3481 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3541 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3601 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3721 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3781 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     3841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4201 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4501 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5761 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5821 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5881 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5941 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6001 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     6061 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6121 tacaaggaga gaaaaagcac cgtgcatggc gattggtgga agtaaggtgg tacgatcgtg
     6181 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     6241 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     6301 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6361 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6421 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6481 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6541 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6601 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6661 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6721 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6781 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6841 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     6901 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     6961 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7021 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7081 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7141 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7201 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7261 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7321 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7381 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7441 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7501 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7561 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7621 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7681 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7741 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     7801 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag
     7861 actagtatcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt
     7921 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa
     7981 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
     8041 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
     8101 tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcttcgaga agacctgttt
     8161 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     8221 ccgagtcggt gcttttttta attctcgacc tcgagacaaa tggcagtatt catccacaat
     8281 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     8341 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     8401 gtttattaca gggacagcag agatccactt tggccgcggc tcgaggcgat aagctttgca
     8461 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     8521 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     8581 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
     8641 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     8701 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     8761 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     8821 ttgcgtgcct tgaattactt ccacctggct
//
