LOCUS       sequence-33067-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP312
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccgggataa cttcgtataa tgtatgctat acgaagttat ggtgaatcat cgataaaaat
       61 aaatatactg agattatagt aagctcattg atcccttaaa ctttcttttc ggtgtatgac
      121 ttatgagggt gagaatgcga aatggcgtgg aaatgtgatc aaaggtaata aaacgtcata
      181 tctatgctac aacattccaa aatttgtccc aaaaagtctt tggttcatga tcttcccata
      241 caattgcctc aatgtctctt ctatcggaat cgatgtcgac atctccaatc ttccaaataa
      301 atctgcatct gaatatgcat tgaaataaga tccaaacagc taagaacagg aaaatagaga
      361 tataggcggc agcaaagcta acaccattga attttggtgc aaaagccgtg aaaccttgaa
      421 taatgataat gatcgtcata aatgtggccg cataataagc caagccgggc attaatttag
      481 ctttaaatgg taactcgtca cgagagatgc cacggtattt caaagcttgc ataaatctga
      541 tgtgcgagat tgagataaat aaccatgcaa aaaagcctgc aacaccagtg atatttaata
      601 gccattcgaa aactttgtca ccaccagtag atgtctccat gtaagccaaa gcgccaaatg
      661 cagcagtaac gaaaactgca atgtatggaa caccaccttt ggtggtcctt gacaggaatt
      721 taggagccaa cttgttcttt gatagaccaa ataaaatacg ggaaccaacg taaatatttg
      781 aatttgcggc agaaataatg gttgttaaga taacagcgtt gaagatatgt ggcaaaacct
      841 ttgtaccaga gttctcaata gcaataataa agggagaagt agaa
//
