LOCUS       sequence-33068-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP314
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccgggataa cttcgtataa tgtatgctat acgaagttat gtatagtact tgtgagagaa
       61 agtaggttta tacataattt tacaactcat tacgcacact catggttttt ggccagcgaa
      121 aacagtttca aaagattgct ggaagtctgc aataatgtca tcaataaatt cgataccaac
      181 agagacacga attaagtcct tggtaacacc agatgccaac ttttctttgt catttaattg
      241 tttgtgggta gtgaagtatg gagcaatgac taaggtcttg gcatcaccaa cattggccaa
      301 gttagaggca agctttaaat tgtcaacaac ttgagcacca gaaagtttga atgggtcagt
      361 ttccttgtcg gcatttggta agtcttttac accgaaagat aagacaccac cgaaaccgtt
      421 agatagatac ttcttagcat tttcatgatg agaatgagat gctaaaccag ggtatgaaac
      481 ccaagatacg tatggggatt gttctaacca tttggctaac ttcaatgcat tttcaccgtg
      541 tctttcagct ctcaaagata atgtttcaac accttgtagt agcaagaaag aggcaaatgg
      601 gttcatcaat ggacccaaat ctcttaatag ttcagttcta acatgaacga tgtatgccaa
      661 gttaccgtag gcttcattgt agatagtacc gtgatatcct tcggcaggtt gagagaattg
      721 agggaacttt tctgggtagt ccttccatgg gaacttacca gagtcaacaa taataccacc
      781 gatagtagta ccatgaccac caatccattt ggtagcagaa tgtgttacaa tatc
//
