LOCUS       sequence-330684-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A C-terminal APEX2 fusion to the human ATGL S47A protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, 
            Nomura DK, Olzmann JA
  TITLE     A Proximity Labeling Strategy Provides Insights into the Composition
            and Dynamics of Lipid Droplet Proteomes.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):97-112.e7. doi: 
            10.1016/j.devcel.2017.11.020. Epub 2017 Dec 21.
  PUBMED    29275994
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.11.020"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "97-112.e7"
COMMENT     Sequence Label: ATGL*-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..47
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(68..814)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
ORIGIN
        1 gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcta gatatctcga
       61 gtcattaggc atcagcaaac ccaagctcgg aaagcttttg gtgagcctca gcgtaatcag
      121 caaagaaggc atcttcgtcc gctgcatatt tatcaacgag agggcggaat acagggtcag
      181 acaaaagagc cttgtcagaa ggtagctgaa ggagaccttc cttctcacca ctcaacaact
      241 ccgtgaagta tgagttgtcg aaaataagag gattagaggt ccagggaccc tcaaatccag
      301 aacgctcctt gtgtgcagct ccaatagtgt gacccccaga tagagcaacg atatcttggt
      361 cagtaagccc catagctttg ccaaacacat ctctcaaatg gtcagaaccc ttagtgggat
      421 cgggcaagcg accctctggt ggtggctcag gcttgtcctc tcttccaggg tggaatggaa
      481 ccttaggtcc acccgtgacc tcaacggcaa caacgccagc caactggtag aaatcggcgt
      541 agctcaaaat agggaactcc gccttgagtg gctccaaaag cctaacagcg atgtcaagac
      601 cgttgttagc gctgtgagcc agttcggcag ggtgcttgat ggttccgaag ggtccaccgg
      661 tcttcgtgcc cttgtcaaag gttccagcag agtggaatgc caaacggagc attagaggag
      721 cgcatctctt ctcagcgatg aagcctctga gcttcttctt cgccttctca acggcgtcct
      781 ggtaatcagc actcacagtt gggtaagact ttccggtgct gtccaggccc agcagggggt
      841 tggggatggg ct
//
