LOCUS       sequence-330690-       11096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD 
            proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS8: pTns(AvCAST)
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(24..1106)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1068..1087
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1107..1184)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1230..1248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1249..1273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        7053..7071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7072..7096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(9071..9089)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9085..9132
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9362..10177)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9362..10177)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10085..10104
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(10178..10269)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10287..10922)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(10432..10451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
       61 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
      121 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
      181 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
      241 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
      301 gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
      361 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
      421 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
      481 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
      541 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
      601 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
      661 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
      721 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
      781 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
      841 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
      901 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
      961 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     1021 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     1081 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     1141 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     1201 tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg aattgtgagc
     1261 ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga tataccatgg
     1321 ttaggggtag aagggaatgg actcaagcta aatatgagcg atatataaaa gaaggtcgtg
     1381 gccgaggaag tggtaagcac tataaacctt ggcttacaat ccaagatgtt ccctctaaag
     1441 gacgttcttc tagaactcct ggttggaaaa caaatcgagt acatcatttc ttctcagacc
     1501 atgaaaaacg gctattttat ttgtttgagt ggtcagatat agtcacagat ataagagagc
     1561 aatttccttt agacaatctc gatctagcca tgagtattgc tacagacata ggtataaagt
     1621 accctgtaga tacacaaagt ggcactcctc acgttttaac aagcgacttt atgcttacag
     1681 tcaaccgcga tgggaagcag atgcagattg cccggactgt taagccgtca acagaactgg
     1741 agaaaaagca agttaatgag aaattcgaga tagaaagaca atattactta gcaaagggta
     1801 ttgactgggg aattatcaca gaaaaagaaa ttcccaggct tttagctgaa aatgttgagt
     1861 gggttcattc tgcttatagg ctagaagcaa attctgaaat ggacactgca caactgagtg
     1921 aattgtcaac aattttaaaa tctaagcttc aggaagaaga tacaactata aatagagtaa
     1981 ctactgcttt agacaaagaa atgaatataa attccggtac atcactttat gtatttaaac
     2041 atttaattgc taggaaagaa atccttgtgg atctagtagc aataaaatta tctgcttgtc
     2101 cgtcaactaa agttattgaa aaaataatat tttagaccca catgataggt aaataatatg
     2161 gttaaagatt tatttgtcaa tgatttaata gagtggattg tataccaaaa cactcatata
     2221 gtcgaaagaa ttatttggat agatgaaggt tacattattg catttgttat agatattaat
     2281 gctaagaaag gtctacctga accaaggaaa atatcagata ttttagaagc tataactgaa
     2341 ggtattgctt ttaagcagca acaagatcct tgggcaagaa ttattcggga tgaaaatttg
     2401 acagataagg aaaaggaata cagagataaa gcttgggaga taatttcatc attaattata
     2461 gaagaaccat caatatatta tagaaatttt agaggatcga tggttaaaga agcggttgaa
     2521 aagtacaacg aagaaaggga taaagataaa ttagtagaaa aaaccgtcta tggttatttg
     2581 agaagatttt ggcagagagg aaaaactata aatgctctca tacctgattt tatcaattca
     2641 tcaggtaagg gcaagattcg aggacaatcg ggcaagaaga gaggaagacc aagaaaatat
     2701 gcacatgacc cagatattgg tgaaggtgta aatgttacag aagaagatag aaagattttt
     2761 agagtatcta taagcaaatt ttacaataat ccaaaagaaa actacttaac aacagcctat
     2821 gaattaatgg tgaaagaata ttacaaggaa ggaattagat atgacgagaa tggtgttcaa
     2881 aaatctatat taaccccaac tgataaaata cctacaatta ctcaattcaa atactggtat
     2941 caacttgaac aggatgatgt taccaaaact ttaacatctc gaaaaggagc caagcgagta
     3001 gctttagaga atagagcaat tttgggaaac tctaagatgg aaactattgg ccctggttct
     3061 agatatcaaa ttgatgccac agttgctgat gtatatttag tttcaaaata taaccgtgac
     3121 tggataattg gtagaccagt tatctatgta attattgatg tttttagtag aatgattaca
     3181 ggtgtttatg tcggtttaga aggcccatct tggataggag caatgatggc actagccaat
     3241 gtagctacag ataaaaccct gttttgccag gaatataaca ttaatattac tgaaaatgaa
     3301 tggccttgca atcaaatgcc agatgcaatt ttaggagata ggggagaatt agcaggtatg
     3361 acagtagaaa cattaatccc taatctttat gttcgtattg aaaatgcagc ttcctataga
     3421 gcagattgga aagggttagt agaaagacac tttcgtataa ttcatgaata tgtaaaacct
     3481 tttttacctg gttatgtgga tacggatttt aggcaaagag gtgcaaggga ttataggctt
     3541 gatggcaaac ttgatataga tcaatttact gagattatta ttcgtttaat tgttcaccat
     3601 aacaataaac ctcttactga atatgaaaga gatgaagcaa tgattgctga tgatacccct
     3661 cctataccta gagagttgtg gaagtggggt atcgccaatc gttctggcag actcagaact
     3721 ttttctgaag atattgtcaa attgaacttg atgcctactg agaaagcaac tattactgct
     3781 aaaggcatca aaatgagagg taaggagatg tactatacct gtgatagtgc taaaaatgag
     3841 caatggtttg aaaaagctag aagtaatctc ctttctaagt cagaaaaatc attgattgta
     3901 tcttatgaca tcagaaagcc aaacttcatt tatcttccat ctagtgatgg tagagatttt
     3961 gaaaaatgtt ttcttattga cttagaaggg cggtatgcta ataaaaactt tcatgatatt
     4021 gaatatctgc ttgcttatga agaattgcaa agacagaaaa gccaaggtaa acagctacaa
     4081 gaaaaggtag atttaatagc tgatattgaa agcatagtag atagagcaga aaaaatgact
     4141 gatgcttctc aatatcataa tttaagtaag agcaagaaag tttctggaat aagagataat
     4201 agggcttttg aaaaggctaa acgacgagag aatgaaggat ttgaattagc aattacagat
     4261 aatcagagca gttttcaggt tgcagagagt acagcagaaa cctccgaaca gccagaatca
     4321 ctacagccaa accatctgaa tcttcttaag aaaaagcgac aggagcgaaa acgtggacag
     4381 aattgagagt gaactggtta aaattcctaa tggagaacta gcagttattg ccagatatat
     4441 agatcaaaaa ctgccagaat acaacactaa tccattaatt caggcattac ctcctatcct
     4501 atcagcagca gaatttatag ataaagtagc cagagaacca aattttgatg aacaagagag
     4561 agagctagaa ccacattata gatttcattg tattgagagg ttatctagat attttgaccc
     4621 tcaaaataaa actcttgaat tacaaaaagt tatttgtacg cttatcatga caggctatct
     4681 gtcaaggaac cttttaaaac ctgaatatgc aaatcgctct aggcaaattt ataatgcaat
     4741 aggtaatgga ggaggtaaaa atttagaaaa ctatgttaat cttcctacat ctgcgtccgg
     4801 cttaactcta attggcccat caggtattgg aaagactaca aatttcaaaa atattcttga
     4861 gctttatccc caaataattt ttcatcctga ttatagtgtt taccaaatag tttggctgcg
     4921 ggtagattgc cctaaaactt taaaggggtt atgtatagat acttttgcag gtatcgataa
     4981 attgttagga actaatttat taaagaaatt taacccagca cgcacatctg aagattatct
     5041 attggctcaa cttgcacaaa cagcacacac tcatcatcta ggagttttgg tgattgatga
     5101 aatacaaaat ttagcaactg caaaaagaaa tagagacgaa atattaaatt ttttagtaaa
     5161 gctcgataat actattggaa ttccagttat tcgtgtaggt acgaatgaag ccattagttt
     5221 tcttcaaggt aatcttagaa atgctagaag aaatactgga gaaggaagta gaatgtggga
     5281 tagaatggta aataatgatg aatggtgtta ctttttagaa ggaatatggg aataccaatg
     5341 gacaaaaaca cctgttcatt tctcggatga aataaaagaa gttttttatg atgaaagcca
     5401 gggaattatt gatattgcta taaaacttta caaaatggtt caatggagag cgatttcaat
     5461 aggtgaccaa gaaattatta cggttgatct gattcgtcaa gtagctaaag aaggattgta
     5521 tttagtaaag ccaatgctag atgcaattag gttaggggac agagagtgga tgattaaata
     5581 taaggatatt gctcctctcg atattacgga atatcacaat agatgtgtat ctgagattga
     5641 tgctaaagat ttgacagaga ttaagagatt agctaaaaag cagcagaatc agcagaaagt
     5701 atctccgaag ctcaattatg taattagtga gctgttgaaa ttggaagtag aatctggtaa
     5761 agcaaaggaa tgtgcagaac aaattgttta tagtcatgaa gatccagaca ttccaattcc
     5821 atctttagtt aataaggcat atgctcttgc tttgcaagga tgtcaagtaa atgagagtct
     5881 tgctaaaaca agtaccagga aagctaaatt aaagacaaag taccaagaga atgatattcg
     5941 gcgaattgtc gagaatgcca agaaagacaa aaaatctgca tacgaggatt tgaaattagc
     6001 aggtataatc aaaaatccag ttgaagattt tatcaatatt tagcttctaa atgcttagtt
     6061 ttttcccaac tctgtatccc gatgaacttt tatatagcac tttagctaga taccatattc
     6121 ggagtggaaa taaaagtttc aagcaaactg acttagagct attcggttac agttcccaac
     6181 aaatatgcaa aattacctta actaataatt taaattattt agtgaaaaac ttacctttac
     6241 gctcccagca aaaagttgag aatttactac aaaatcatac tttatattcc ttttatgcaa
     6301 ctttattaat gccgcaggag gcttggctgc tgaaaaactc aatgagtaat aaattcagtg
     6361 ggtctatact agattttgct aaagtggcta ataattctag tgatgattca aaaaaatttt
     6421 taaaattttg ccctgaatgt ttagagaaag atacccaaaa gtatggtgaa ccttactggc
     6481 atagaataca tcaagtccct ggcattttag tttgtccaat tcatcgtgtt gttttacaca
     6541 atagctgtat agaagttgaa tcgaaaggca tacattatca tgctgccagc atagataatt
     6601 gtttattagc aaataatgtg aaaaaattga caaataaaac tgtagaaaca ctttcaatac
     6661 tggcacatga tattaattgg ctaattcata gcagttttgc ctttcaaggt ttggcttggc
     6721 ttcgcgctca gtaccagcgt tacttattgg ataaaggact gctaaaaacc tttgctggtg
     6781 gcaagtttaa gtttaatgat tgtggttttg cccagcttat atttgagttt tatggtcaag
     6841 aatccttaga agccctcaat ccaaagctga cccaaaatca aggtaaatat ttttctcact
     6901 gcttgtttgg atgcgactta aacccagcaa ttgaccgcat aactcatatc ttaatgataa
     6961 aatttttagc aaattctata gaagagttct ttcagtcata acttaagtcg aacagaaagt
     7021 aatcgtattg tacacggccg cataatcgaa attaatacga ctcactatag gggaattgtg
     7081 agcggataac aattccccat cttagtatat tagttaagta taagaaggag atatacatgg
     7141 tgagtttttt ccctcaacct tatccagatg aaattttata tagtgttatt gctcgttatc
     7201 acattagaag tggcaatacg agtccaaaaa ttacattaca agatttattt aagtcacaga
     7261 ctactgtggc gacagtagat ttaccatcga accttagtaa tttaattaaa aacctacaat
     7321 ttatctcaaa ttaccaagtt gaagagttga tatataaaca tactctctac cctttatata
     7381 gccctttttt gccagcaaac aggtctagtc aggtactgga atctatgaaa ggagattatg
     7441 ggggagatat tcatacaaga gccggaatta tggcaagctc aattactatg ccgaaatatt
     7501 tgcggttttg tccaacttgc tgggaagaag acttaaaaaa ttatggcgaa ccttactggc
     7561 atcgcatcca tcaaatacca gggattttag tttgtccatt tcatggtgaa gttttgcaag
     7621 atagcctagc tcctttacat ggttttaata agcatgaata ttatgcagct agtacagaca
     7681 actgttctat tactaaagaa cccagtattc ttagccctga cacactgaaa aaattactaa
     7741 tattagccaa agatttttgg tggttactca atagtgaaat accttgccaa gagccacagt
     7801 ggtttcgcaa acagtacaca aatctgatga ttgagcaggg ttttgcaact ccaactggtc
     7861 gaatttacca gaacaaacta ctagataatt ttttgttctt ttatggtcaa gaaatgctca
     7921 gtgctgttga ttcaatggta aatcataatg attcaaacaa ctgggttact agcatcgtca
     7981 ggaaatatag gaaatctttc catcctctta gacatctgct aatgatgcgt tttttgaaaa
     8041 catcagtaga ggaatttttt caaaaagata ctgaatacaa gccttttggc gaacccccct
     8101 ggttgtgtct caatgctgcc gcagaacatt atctacaacc agtgattacc aatttagtca
     8161 ttactttatg ctgcgataca aaaaagcctg tcggtacttt ttcttgtgct tgcggaatgg
     8221 tttattgccg gactgggcct gatgaaacgg atgaagacaa agttcgcatt ggtaaagtca
     8281 aggcattcgg tcaaatatgg gagcaaaagc tgaaagaatt ggtggaagtt gaaaagttgg
     8341 gtttgcggga gacagccaga agattaaatg ttgatgccag aactatcaaa cgttatgtat
     8401 ctcgtctaaa attgacaact tattggcaga ctagaacaga aaatgactct gtaggcttac
     8461 agggagtccc agaaatagat cccaattttg caactgaact caaattaaaa cacaggcaaa
     8521 attggatggc tttacaagta cagaaccctg aagcttcaaa gacaactctg agaaaactag
     8581 taccagccac ctatatttgg ctatacagga atgacagaga atggctcaat caaaactcac
     8641 ccactttaca agttgccgtt ccttccgttg tgagagttga ttggcatgag cgagatcagc
     8701 aaattctcgc tcaggttcaa gatgcagtgc gatcgctact taaggctgat ataccagaga
     8761 aaatttctat tagtagggta gggaaaacta tcggcttact tgcattactc gaaaaacatc
     8821 tcgaccagat gcctttaaca aaggcttatt tggaatcagt cgttgaaact gtagaagaat
     8881 ttcagatgcg ccggatcaag tgggcaatca agctgcttga tgaatgtgga gaagaaatta
     8941 tgcgctggaa ggtagtcaga gttgctgctt tgcgagagga ttgctcccaa agagtcagtg
     9001 catttctgga aagtgaactg gagaaagcat acaagaaaga gaggtgatta attaacctag
     9061 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     9121 aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta
     9181 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     9241 ccgacgacaa gctgacgacc gggtctccgc aagtggcact tttcggggaa atgtgcgcgg
     9301 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
     9361 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     9421 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     9481 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     9541 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     9601 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     9661 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     9721 gcgcctgagc gagacgaaat acgcggtcgc tgttaaaagg acaattacaa acaggaatcg
     9781 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     9841 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     9901 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     9961 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
    10021 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
    10081 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
    10141 gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt
    10201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10261 ataaacaaat aggcatgcta gcgcagaaac gtcctagaag atgccaggag gatacttagc
    10321 agagagacaa taaggccgga gcgaagccgt ttttccatag gctccgcccc cctgacgaac
    10381 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
    10441 aggcgtttcc ccctgatggc tccctcttgc gctctcctgt tcccgtcctg cggcgtccgt
    10501 gttgtggtgg aggctttacc caaatcacca cgtcccgttc cgtgtagaca gttcgctcca
    10561 agctgggctg tgtgcaagaa ccccccgttc agcccgactg ctgcgcctta tccggtaact
    10621 atcatcttga gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattggta
    10681 actgagaatt agtggattta gatatcgaga gtcttgaagt ggtggcctaa cagaggctac
    10741 actgaaagga cagtatttgg tatctgcgct ccactaaagc cagttaccag gttaagcagt
    10801 tccccaactg acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacaga
    10861 gcaggagatt acgacgatcg taaaaggatc tcaagaagat cctttacgga ttcccgacac
    10921 catcactcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
    10981 tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga aggagctgac
    11041 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagct
//
