LOCUS       sequence-330692-       14803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins (except TniQ). Two BsaI 
            sites for spacer cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 14803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS5: pHelper(AvCAST)_entry_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..14803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     970..989
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1009..1086)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1132..1150
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1151..1175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1414..1432
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1433..1457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5436..5483
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5632..5650
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5651..5675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10504..10522
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10523..10547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12536..12583
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12785..13576)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13577..13668)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(14729..14803,1..1008))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
       61 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
      121 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
      181 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
      241 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
      301 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
      361 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
      421 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
      481 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
      541 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
      601 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
      661 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
      721 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
      781 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
      841 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
      901 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
      961 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     1021 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     1081 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac
     1141 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
     1201 ttaataagga gatataccat ggtgctttaa cattagatgt cgttaggcgt tgagcagaga
     1261 cctctggtct cgtgctttaa cattagatgt cgttaggcgt tgagcagatc cgaattcgag
     1321 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
     1381 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
     1441 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
     1501 tgattattga tagtaactta gttacgcagc ttccctcaga atcatcttca aagccaagag
     1561 agcttttaat gtctcagttt ccatatatgg atttaacttt tagccttacg ggtgagacat
     1621 tacctttcga ccacggctat gaattatttt ctgcgatcgc ccactttgaa cccaaactac
     1681 atacattaga cacgttggga attcagacca ttgcaggtat tcccaaggat ggtgtaatta
     1741 atctgaccca aaattctagg cttcgtattc ggataccagt aaatcaggta cgtttggttt
     1801 atccattggc gggtaagtca ctgagaattg gcaagcatac aattcgtttg ggtattcctg
     1861 acatctgttt gcttcaacct gcggagaaac tgcgatcgcg cattgtggta ataagaggct
     1921 atgaagaacc tgaaagtttt ttagtcgtag cgcagcgtca gctagaacaa ctgggtatcc
     1981 aagcaattgc tagcattccc accaaagcca atggcaaacc aatacgtagg actatcaaaa
     2041 ttaagcgatt tacagtcgtg ggttttggat tagaagttat aaatttaagc gatgaagact
     2101 cactgaggtt gcaaatacat ggtgtaggag gaaagcaaaa gatgggttgt ggactcttca
     2161 tgcctattaa ggaaaggcaa tagcaattac tcgaaagcag atgatgaaga gagcagataa
     2221 ccccaaaatt cagcttaatc tcggcgatcc tagtatgact ttattgcatc gtgctggggt
     2281 tgcaggactt tggatgacgc tcaaaaaact ggaaaaaatc tatccaacac ctgctgaacg
     2341 tattggtaat ttaacttggt tgctaacccc tcactatatc aatcttgatt gggaaggaca
     2401 ggatttcaca gttttagact ggttgctgaa gcaatcattt caaatcagcg atgagggttt
     2461 aatttccctg acagggttag attctcaaaa catggatatt caaaatcaaa taattatcca
     2521 tcaaggtatc acagccactt ttacacaaca taataaattt tttaagtctg cgggtcaaca
     2581 atctaagcaa ctaattattg atggtattca ggttagggtt gattataaga aagctgcttc
     2641 ctatgctcat caacattttg ctaaacattt atgtgaccag tacggacaat tgctaaaaga
     2701 gccaattggt atcaggggat ggctctatcc aggagcagta gttcgccatt atgcttttaa
     2761 agaacaaaca gcatttgaag aaaaagcaga atatgctcta gcgttgttgt tcgctcctat
     2821 ggcttgtcaa tattttgtgc tgcgatcgca caccgaacca tcacatacaa actatgtttt
     2881 ggttattcct gaagtaattg atttagaact ttatgcacat tttttgtggt gtttgggtaa
     2941 cttagattat aaacattttc acgtatctag cttaggagat gctggattaa aatttcttaa
     3001 ttataaaaca acacaaatgc ctacttccaa ttttttaaaa cgatgtcaaa taatcttttt
     3061 tagtacaaaa gcttggtcag aacagcaaaa aactcgtgca gaaatagcaa tcatagaatt
     3121 aaattcttca gttaattatc tttataagct tagttgtatt tgttttccag aatgtcaaat
     3181 tgttttatat aaaaatcaac attatttgct tccaagttta ataaaaggaa taatcgccaa
     3241 taatctagca atgggtcggt cttggtgggc taattggatt tcatttatta aaaataaaaa
     3301 atcatttaaa caatttactg atgagtataa aggaattaat aagatgattc aggactcaga
     3361 atgggatata gaagctcaaa agctatttat tcaagcttgt catgaagcat taagaaaaat
     3421 atatgccaaa atttatagca gaacaaaaga aggtgaatac gctcaaatag aacgagaaaa
     3481 tacacgcatt ttatctcagt taaaacgctg caccaatact gacaatttcc gaaaatttat
     3541 tgcagagttt tggggaaggg cagggcaaat agcaattctg gaagaacatt ggatggaatt
     3601 acttccattg actacaggga taggcaattg gaaagtggcc agggacttaa cttttattgc
     3661 aatggctagc tacccaaaga ataaaataac agaggtagag gtaaaaccat cacaaatatc
     3721 ggagctaaat actgaataaa cttcatattt tttgatttga ttaagaacaa aatattaatt
     3781 gtttaggtaa tggtggaatg tttcatctgt ttggtaatat tttgactagt tatggaaccg
     3841 ccgctaataa tcgtggtgaa aatgaaggta ataccactac gttacaaaaa ttaatttgga
     3901 aaggtgaagt tcactctact gtttcatctg aggcaattcg ttgggcattg cgatattatt
     3961 ggcaaaatgc tggctatcct gtgaatagac gttgggatga aaatgctcaa ccagtacccg
     4021 atcacatctt gcaagatcct aattttgatg atattcgctt cattgatgat gatgtcttgg
     4081 gatttatgca agcggaagca gcaaaagttg aagcagcagt tgaatccgaa gttgaaatcc
     4141 agaactcaac tcaagatgag gacaaaaaac tttctcaagg taaggagaaa gaaactgcaa
     4201 aacgtaagcg taaggaaaaa cctaaaggga aaataactac tagacgaggt gtattagaag
     4261 tcactcgtgc agtttcaaca attccttatg ctggtgatct tactttcaat gctatgagtg
     4321 gaaagaaagg acgaacatct ctttattcta cagaagttca tgctactcga tatcaatatg
     4381 gcttcgcatt aacacccaat agcctcaaag ataaatctcg cattaatgct gtcctcgata
     4441 gcttaatttc tattggagaa gtggcaggaa atcacgctcg ttttctctat gatttttctc
     4501 ctgatagcat catattacgg tggacacacg atttctcacc tcggttactt tactgttttg
     4561 aagaagatga acttagaaat atatcaacac cggatttagt aaggcgggta gaatctggag
     4621 atatagaccc taaagaatta tggataggag gggcaatttc tagcactctg gaagatttag
     4681 gggcaaatgt gtttcccgga gtgaagacaa cagttgaagc attaaaacag gtgattattg
     4741 aagatttgca gttgtcgtta aggagttcat gatatgacga caattgcctt aaaagtagaa
     4801 gttcccatag cttgttttcg gcagtctcgt gctagggaat atggcgaaac ttaccctgta
     4861 ccaccaccat cgacagtata tggaatgtta ctatctcttg ttggagaagt ggatagatac
     4921 aaacactgcg gtgtgaagct ggcgatcgca cttctatctg aaccagaaaa gtcaactgtt
     4981 attcgcacct ttcaccgatt taagacgaag aatatccatg actctaaaaa taataaacct
     5041 gattatcagg agttattaac taatatagaa tttatagttt gggtggatgc tggagtagat
     5101 aaagctaaac caaatttagt ggaacgttta gcagaggcat tgactaaccc ggcttctatt
     5161 aataggtttg gtggattatg tttaggtgaa agtcgggatt tagtgaatga tgttaccctt
     5221 ttacctaaaa attactatgc agaatcaatg cgatggttaa ttcgagatga atatgggtta
     5281 ctaactttac cttactgggt agaccatgta ggttcgcggg gaacgcgatg gctacgttac
     5341 gagattcaga aatgtccagt ctttcaacca cctgaattat cttggacttc agttcaaagt
     5401 aattagccta ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     5461 aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac
     5521 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
     5581 cctgccctgg atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac
     5641 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
     5701 ttaataagga gatataccat ggttaggggt agaagggaat ggactcaagc taaatatgag
     5761 cgatatataa aagaaggtcg tggccgagga agtggtaagc actataaacc ttggcttaca
     5821 atccaagatg ttccctctaa aggacgttct tctagaactc ctggttggaa aacaaatcga
     5881 gtacatcatt tcttctcaga ccatgaaaaa cggctatttt atttgtttga gtggtcagat
     5941 atagtcacag atataagaga gcaatttcct ttagacaatc tcgatctagc catgagtatt
     6001 gctacagaca taggtataaa gtaccctgta gatacacaaa gtggcactcc tcacgtttta
     6061 acaagcgact ttatgcttac agtcaaccgc gatgggaagc agatgcagat tgcccggact
     6121 gttaagccgt caacagaact ggagaaaaag caagttaatg agaaattcga gatagaaaga
     6181 caatattact tagcaaaggg tattgactgg ggaattatca cagaaaaaga aattcccagg
     6241 cttttagctg aaaatgttga gtgggttcat tctgcttata ggctagaagc aaattctgaa
     6301 atggacactg cacaactgag tgaattgtca acaattttaa aatctaagct tcaggaagaa
     6361 gatacaacta taaatagagt aactactgct ttagacaaag aaatgaatat aaattccggt
     6421 acatcacttt atgtatttaa acatttaatt gctaggaaag aaatccttgt ggatctagta
     6481 gcaataaaat tatctgcttg tccgtcaact aaagttattg aaaaaataat attttagacc
     6541 cacatgatag gtaaataata tggttaaaga tttatttgtc aatgatttaa tagagtggat
     6601 tgtataccaa aacactcata tagtcgaaag aattatttgg atagatgaag gttacattat
     6661 tgcatttgtt atagatatta atgctaagaa aggtctacct gaaccaagga aaatatcaga
     6721 tattttagaa gctataactg aaggtattgc ttttaagcag caacaagatc cttgggcaag
     6781 aattattcgg gatgaaaatt tgacagataa ggaaaaggaa tacagagata aagcttggga
     6841 gataatttca tcattaatta tagaagaacc atcaatatat tatagaaatt ttagaggatc
     6901 gatggttaaa gaagcggttg aaaagtacaa cgaagaaagg gataaagata aattagtaga
     6961 aaaaaccgtc tatggttatt tgagaagatt ttggcagaga ggaaaaacta taaatgctct
     7021 catacctgat tttatcaatt catcaggtaa gggcaagatt cgaggacaat cgggcaagaa
     7081 gagaggaaga ccaagaaaat atgcacatga cccagatatt ggtgaaggtg taaatgttac
     7141 agaagaagat agaaagattt ttagagtatc tataagcaaa ttttacaata atccaaaaga
     7201 aaactactta acaacagcct atgaattaat ggtgaaagaa tattacaagg aaggaattag
     7261 atatgacgag aatggtgttc aaaaatctat attaacccca actgataaaa tacctacaat
     7321 tactcaattc aaatactggt atcaacttga acaggatgat gttaccaaaa ctttaacatc
     7381 tcgaaaagga gccaagcgag tagctttaga gaatagagca attttgggaa actctaagat
     7441 ggaaactatt ggccctggtt ctagatatca aattgatgcc acagttgctg atgtatattt
     7501 agtttcaaaa tataaccgtg actggataat tggtagacca gttatctatg taattattga
     7561 tgtttttagt agaatgatta caggtgttta tgtcggttta gaaggcccat cttggatagg
     7621 agcaatgatg gcactagcca atgtagctac agataaaacc ctgttttgcc aggaatataa
     7681 cattaatatt actgaaaatg aatggccttg caatcaaatg ccagatgcaa ttttaggaga
     7741 taggggagaa ttagcaggta tgacagtaga aacattaatc cctaatcttt atgttcgtat
     7801 tgaaaatgca gcttcctata gagcagattg gaaagggtta gtagaaagac actttcgtat
     7861 aattcatgaa tatgtaaaac cttttttacc tggttatgtg gatacggatt ttaggcaaag
     7921 aggtgcaagg gattataggc ttgatggcaa acttgatata gatcaattta ctgagattat
     7981 tattcgttta attgttcacc ataacaataa acctcttact gaatatgaaa gagatgaagc
     8041 aatgattgct gatgataccc ctcctatacc tagagagttg tggaagtggg gtatcgccaa
     8101 tcgttctggc agactcagaa ctttttctga agatattgtc aaattgaact tgatgcctac
     8161 tgagaaagca actattactg ctaaaggcat caaaatgaga ggtaaggaga tgtactatac
     8221 ctgtgatagt gctaaaaatg agcaatggtt tgaaaaagct agaagtaatc tcctttctaa
     8281 gtcagaaaaa tcattgattg tatcttatga catcagaaag ccaaacttca tttatcttcc
     8341 atctagtgat ggtagagatt ttgaaaaatg ttttcttatt gacttagaag ggcggtatgc
     8401 taataaaaac tttcatgata ttgaatatct gcttgcttat gaagaattgc aaagacagaa
     8461 aagccaaggt aaacagctac aagaaaaggt agatttaata gctgatattg aaagcatagt
     8521 agatagagca gaaaaaatga ctgatgcttc tcaatatcat aatttaagta agagcaagaa
     8581 agtttctgga ataagagata atagggcttt tgaaaaggct aaacgacgag agaatgaagg
     8641 atttgaatta gcaattacag ataatcagag cagttttcag gttgcagaga gtacagcaga
     8701 aacctccgaa cagccagaat cactacagcc aaaccatctg aatcttctta agaaaaagcg
     8761 acaggagcga aaacgtggac agaattgaga gtgaactggt taaaattcct aatggagaac
     8821 tagcagttat tgccagatat atagatcaaa aactgccaga atacaacact aatccattaa
     8881 ttcaggcatt acctcctatc ctatcagcag cagaatttat agataaagta gccagagaac
     8941 caaattttga tgaacaagag agagagctag aaccacatta tagatttcat tgtattgaga
     9001 ggttatctag atattttgac cctcaaaata aaactcttga attacaaaaa gttatttgta
     9061 cgcttatcat gacaggctat ctgtcaagga accttttaaa acctgaatat gcaaatcgct
     9121 ctaggcaaat ttataatgca ataggtaatg gaggaggtaa aaatttagaa aactatgtta
     9181 atcttcctac atctgcgtcc ggcttaactc taattggccc atcaggtatt ggaaagacta
     9241 caaatttcaa aaatattctt gagctttatc cccaaataat ttttcatcct gattatagtg
     9301 tttaccaaat agtttggctg cgggtagatt gccctaaaac tttaaagggg ttatgtatag
     9361 atacttttgc aggtatcgat aaattgttag gaactaattt attaaagaaa tttaacccag
     9421 cacgcacatc tgaagattat ctattggctc aacttgcaca aacagcacac actcatcatc
     9481 taggagtttt ggtgattgat gaaatacaaa atttagcaac tgcaaaaaga aatagagacg
     9541 aaatattaaa ttttttagta aagctcgata atactattgg aattccagtt attcgtgtag
     9601 gtacgaatga agccattagt tttcttcaag gtaatcttag aaatgctaga agaaatactg
     9661 gagaaggaag tagaatgtgg gatagaatgg taaataatga tgaatggtgt tactttttag
     9721 aaggaatatg ggaataccaa tggacaaaaa cacctgttca tttctcggat gaaataaaag
     9781 aagtttttta tgatgaaagc cagggaatta ttgatattgc tataaaactt tacaaaatgg
     9841 ttcaatggag agcgatttca ataggtgacc aagaaattat tacggttgat ctgattcgtc
     9901 aagtagctaa agaaggattg tatttagtaa agccaatgct agatgcaatt aggttagggg
     9961 acagagagtg gatgattaaa tataaggata ttgctcctct cgatattacg gaatatcaca
    10021 atagatgtgt atctgagatt gatgctaaag atttgacaga gattaagaga ttagctaaaa
    10081 agcagcagaa tcagcagaaa gtatctccga agctcaatta tgtaattagt gagctgttga
    10141 aattggaagt agaatctggt aaagcaaagg aatgtgcaga acaaattgtt tatagtcatg
    10201 aagatccaga cattccaatt ccatctttag ttaataaggc atatgctctt gctttgcaag
    10261 gatgtcaagt aaatgagagt cttgctaaaa caagtaccag gaaagctaaa ttaaagacaa
    10321 agtaccaaga gaatgatatt cggcgaattg tcgagaatgc caagaaagac aaaaaatctg
    10381 catacgagga tttgaaatta gcaggtataa tcaaaaatcc agttgaagat tttatcaata
    10441 tttagcttct aacttaagtc gaacagaaag taatcgtatt gtacacggcc gcataatcga
    10501 aattaatacg actcactata ggggaattgt gagcggataa caattcccca tcttagtata
    10561 ttagttaagt ataagaagga gatatacatg gtgagttttt tccctcaacc ttatccagat
    10621 gaaattttat atagtgttat tgctcgttat cacattagaa gtggcaatac gagtccaaaa
    10681 attacattac aagatttatt taagtcacag actactgtgg cgacagtaga tttaccatcg
    10741 aaccttagta atttaattaa aaacctacaa tttatctcaa attaccaagt tgaagagttg
    10801 atatataaac atactctcta ccctttatat agcccttttt tgccagcaaa caggtctagt
    10861 caggtactgg aatctatgaa aggagattat gggggagata ttcatacaag agccggaatt
    10921 atggcaagct caattactat gccgaaatat ttgcggtttt gtccaacttg ctgggaagaa
    10981 gacttaaaaa attatggcga accttactgg catcgcatcc atcaaatacc agggatttta
    11041 gtttgtccat ttcatggtga agttttgcaa gatagcctag ctcctttaca tggttttaat
    11101 aagcatgaat attatgcagc tagtacagac aactgttcta ttactaaaga acccagtatt
    11161 cttagccctg acacactgaa aaaattacta atattagcca aagatttttg gtggttactc
    11221 aatagtgaaa taccttgcca agagccacag tggtttcgca aacagtacac aaatctgatg
    11281 attgagcagg gttttgcaac tccaactggt cgaatttacc agaacaaact actagataat
    11341 tttttgttct tttatggtca agaaatgctc agtgctgttg attcaatggt aaatcataat
    11401 gattcaaaca actgggttac tagcatcgtc aggaaatata ggaaatcttt ccatcctctt
    11461 agacatctgc taatgatgcg ttttttgaaa acatcagtag aggaattttt tcaaaaagat
    11521 actgaataca agccttttgg cgaacccccc tggttgtgtc tcaatgctgc cgcagaacat
    11581 tatctacaac cagtgattac caatttagtc attactttat gctgcgatac aaaaaagcct
    11641 gtcggtactt tttcttgtgc ttgcggaatg gtttattgcc ggactgggcc tgatgaaacg
    11701 gatgaagaca aagttcgcat tggtaaagtc aaggcattcg gtcaaatatg ggagcaaaag
    11761 ctgaaagaat tggtggaagt tgaaaagttg ggtttgcggg agacagccag aagattaaat
    11821 gttgatgcca gaactatcaa acgttatgta tctcgtctaa aattgacaac ttattggcag
    11881 actagaacag aaaatgactc tgtaggctta cagggagtcc cagaaataga tcccaatttt
    11941 gcaactgaac tcaaattaaa acacaggcaa aattggatgg ctttacaagt acagaaccct
    12001 gaagcttcaa agacaactct gagaaaacta gtaccagcca cctatatttg gctatacagg
    12061 aatgacagag aatggctcaa tcaaaactca cccactttac aagttgccgt tccttccgtt
    12121 gtgagagttg attggcatga gcgagatcag caaattctcg ctcaggttca agatgcagtg
    12181 cgatcgctac ttaaggctga tataccagag aaaatttcta ttagtagggt agggaaaact
    12241 atcggcttac ttgcattact cgaaaaacat ctcgaccaga tgcctttaac aaaggcttat
    12301 ttggaatcag tcgttgaaac tgtagaagaa tttcagatgc gccggatcaa gtgggcaatc
    12361 aagctgcttg atgaatgtgg agaagaaatt atgcgctgga aggtagtcag agttgctgct
    12421 ttgcgagagg attgctccca aagagtcagt gcatttctgg aaagtgaact ggagaaagca
    12481 tacaagaaag agaggtgatt aattaaccta ggctgctgcc accgctgagc aataactagc
    12541 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt
    12601 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
    12661 ataagcggct atttaacgac cctgccctga accgacgaca agctgacgac cgctgccctg
    12721 aaccgacgac cgggtcatcg tggccggatc ttgcggcccc tcggcttgaa cgaattgtta
    12781 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
    12841 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
    12901 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
    12961 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
    13021 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
    13081 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
    13141 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
    13201 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
    13261 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
    13321 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
    13381 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
    13441 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
    13501 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
    13561 tcaccgcttc cctcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13621 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata gctagctcac
    13681 tcggtcgcta cgctccgggc gtgagactgc ggcgggcgct gcggacacat acaaagttac
    13741 ccacagattc cgtggataag caggggacta acatgtgagg caaaacagca gggccgcgcc
    13801 ggtggcgttt ttccataggc tccgccctcc tgccagagtt cacataaaca gacgcttttc
    13861 cggtgcatct gtgggagccg tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg
    13921 acaggactta aagatcccca ccgtttccgg cgggtcgctc cctcttgcgc tctcctgttc
    13981 cgaccctgcc gtttaccgga tacctgttcc gcctttctcc cttacgggaa gtgtggcgct
    14041 ttctcatagc tcacacactg gtatctcggc tcggtgtagg tcgttcgctc caagctgggc
    14101 tgtaagcaag aactccccgt tcagcccgac tgctgcgcct tatccggtaa ctgttcactt
    14161 gagtccaacc cggaaaagca cggtaaaacg ccactggcag cagccattgg taactgggag
    14221 ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg
    14281 gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta ccacggttaa
    14341 gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt tttttcgaag
    14401 gttaagtcag ttggggaact gcttaacctt cgatcaaacc acctccccag gtggtttttt
    14461 cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    14521 tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
    14581 tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg cccaccggaa
    14641 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
    14701 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    14761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggc
//
