LOCUS       sequence-330693-       13527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins (except TnsD) with crRNA 
            targeting AvPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS3: pHelper(AvCAST)_AvPSP1_Delta-TnsD
FEATURES             Location/Qualifiers
     source          1..13527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..116
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    117..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4106..4124)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4120..4167
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4316..4334
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4335..4359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(10171..10962)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(10963..11054)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(12115..13197)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13159..13178
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13198..13275)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        13321..13339
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    13340..13364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta agtcgaacag
       61 aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggggaa
      121 ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga aggagatata
      181 catatgatta ttgatagtaa cttagttacg cagcttccct cagaatcatc ttcaaagcca
      241 agagagcttt taatgtctca gtttccatat atggatttaa cttttagcct tacgggtgag
      301 acattacctt tcgaccacgg ctatgaatta ttttctgcga tcgcccactt tgaacccaaa
      361 ctacatacat tagacacgtt gggaattcag accattgcag gtattcccaa ggatggtgta
      421 attaatctga cccaaaattc taggcttcgt attcggatac cagtaaatca ggtacgtttg
      481 gtttatccat tggcgggtaa gtcactgaga attggcaagc atacaattcg tttgggtatt
      541 cctgacatct gtttgcttca acctgcggag aaactgcgat cgcgcattgt ggtaataaga
      601 ggctatgaag aacctgaaag ttttttagtc gtagcgcagc gtcagctaga acaactgggt
      661 atccaagcaa ttgctagcat tcccaccaaa gccaatggca aaccaatacg taggactatc
      721 aaaattaagc gatttacagt cgtgggtttt ggattagaag ttataaattt aagcgatgaa
      781 gactcactga ggttgcaaat acatggtgta ggaggaaagc aaaagatggg ttgtggactc
      841 ttcatgccta ttaaggaaag gcaatagcaa ttactcgaaa gcagatgatg aagagagcag
      901 ataaccccaa aattcagctt aatctcggcg atcctagtat gactttattg catcgtgctg
      961 gggttgcagg actttggatg acgctcaaaa aactggaaaa aatctatcca acacctgctg
     1021 aacgtattgg taatttaact tggttgctaa cccctcacta tatcaatctt gattgggaag
     1081 gacaggattt cacagtttta gactggttgc tgaagcaatc atttcaaatc agcgatgagg
     1141 gtttaatttc cctgacaggg ttagattctc aaaacatgga tattcaaaat caaataatta
     1201 tccatcaagg tatcacagcc acttttacac aacataataa attttttaag tctgcgggtc
     1261 aacaatctaa gcaactaatt attgatggta ttcaggttag ggttgattat aagaaagctg
     1321 cttcctatgc tcatcaacat tttgctaaac atttatgtga ccagtacgga caattgctaa
     1381 aagagccaat tggtatcagg ggatggctct atccaggagc agtagttcgc cattatgctt
     1441 ttaaagaaca aacagcattt gaagaaaaag cagaatatgc tctagcgttg ttgttcgctc
     1501 ctatggcttg tcaatatttt gtgctgcgat cgcacaccga accatcacat acaaactatg
     1561 ttttggttat tcctgaagta attgatttag aactttatgc acattttttg tggtgtttgg
     1621 gtaacttaga ttataaacat tttcacgtat ctagcttagg agatgctgga ttaaaatttc
     1681 ttaattataa aacaacacaa atgcctactt ccaatttttt aaaacgatgt caaataatct
     1741 tttttagtac aaaagcttgg tcagaacagc aaaaaactcg tgcagaaata gcaatcatag
     1801 aattaaattc ttcagttaat tatctttata agcttagttg tatttgtttt ccagaatgtc
     1861 aaattgtttt atataaaaat caacattatt tgcttccaag tttaataaaa ggaataatcg
     1921 ccaataatct agcaatgggt cggtcttggt gggctaattg gatttcattt attaaaaata
     1981 aaaaatcatt taaacaattt actgatgagt ataaaggaat taataagatg attcaggact
     2041 cagaatggga tatagaagct caaaagctat ttattcaagc ttgtcatgaa gcattaagaa
     2101 aaatatatgc caaaatttat agcagaacaa aagaaggtga atacgctcaa atagaacgag
     2161 aaaatacacg cattttatct cagttaaaac gctgcaccaa tactgacaat ttccgaaaat
     2221 ttattgcaga gttttgggga agggcagggc aaatagcaat tctggaagaa cattggatgg
     2281 aattacttcc attgactaca gggataggca attggaaagt ggccagggac ttaactttta
     2341 ttgcaatggc tagctaccca aagaataaaa taacagaggt agaggtaaaa ccatcacaaa
     2401 tatcggagct aaatactgaa taaacttcat attttttgat ttgattaaga acaaaatatt
     2461 aattgtttag gtaatggtgg aatgtttcat ctgtttggta atattttgac tagttatgga
     2521 accgccgcta ataatcgtgg tgaaaatgaa ggtaatacca ctacgttaca aaaattaatt
     2581 tggaaaggtg aagttcactc tactgtttca tctgaggcaa ttcgttgggc attgcgatat
     2641 tattggcaaa atgctggcta tcctgtgaat agacgttggg atgaaaatgc tcaaccagta
     2701 cccgatcaca tcttgcaaga tcctaatttt gatgatattc gcttcattga tgatgatgtc
     2761 ttgggattta tgcaagcgga agcagcaaaa gttgaagcag cagttgaatc cgaagttgaa
     2821 atccagaact caactcaaga tgaggacaaa aaactttctc aaggtaagga gaaagaaact
     2881 gcaaaacgta agcgtaagga aaaacctaaa gggaaaataa ctactagacg aggtgtatta
     2941 gaagtcactc gtgcagtttc aacaattcct tatgctggtg atcttacttt caatgctatg
     3001 agtggaaaga aaggacgaac atctctttat tctacagaag ttcatgctac tcgatatcaa
     3061 tatggcttcg cattaacacc caatagcctc aaagataaat ctcgcattaa tgctgtcctc
     3121 gatagcttaa tttctattgg agaagtggca ggaaatcacg ctcgttttct ctatgatttt
     3181 tctcctgata gcatcatatt acggtggaca cacgatttct cacctcggtt actttactgt
     3241 tttgaagaag atgaacttag aaatatatca acaccggatt tagtaaggcg ggtagaatct
     3301 ggagatatag accctaaaga attatggata ggaggggcaa tttctagcac tctggaagat
     3361 ttaggggcaa atgtgtttcc cggagtgaag acaacagttg aagcattaaa acaggtgatt
     3421 attgaagatt tgcagttgtc gttaaggagt tcatgatatg acgacaattg ccttaaaagt
     3481 agaagttccc atagcttgtt ttcggcagtc tcgtgctagg gaatatggcg aaacttaccc
     3541 tgtaccacca ccatcgacag tatatggaat gttactatct cttgttggag aagtggatag
     3601 atacaaacac tgcggtgtga agctggcgat cgcacttcta tctgaaccag aaaagtcaac
     3661 tgttattcgc acctttcacc gatttaagac gaagaatatc catgactcta aaaataataa
     3721 acctgattat caggagttat taactaatat agaatttata gtttgggtgg atgctggagt
     3781 agataaagct aaaccaaatt tagtggaacg tttagcagag gcattgacta acccggcttc
     3841 tattaatagg tttggtggat tatgtttagg tgaaagtcgg gatttagtga atgatgttac
     3901 ccttttacct aaaaattact atgcagaatc aatgcgatgg ttaattcgag atgaatatgg
     3961 gttactaact ttaccttact gggtagacca tgtaggttcg cggggaacgc gatggctacg
     4021 ttacgagatt cagaaatgtc cagtctttca accacctgaa ttatcttgga cttcagttca
     4081 aagtaattag cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     4141 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
     4201 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     4261 cgaccctgcc ctggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata
     4321 cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt
     4381 aactttaata aggagatata ccatggttag gggtagaagg gaatggactc aagctaaata
     4441 tgagcgatat ataaaagaag gtcgtggccg aggaagtggt aagcactata aaccttggct
     4501 tacaatccaa gatgttccct ctaaaggacg ttcttctaga actcctggtt ggaaaacaaa
     4561 tcgagtacat catttcttct cagaccatga aaaacggcta ttttatttgt ttgagtggtc
     4621 agatatagtc acagatataa gagagcaatt tcctttagac aatctcgatc tagccatgag
     4681 tattgctaca gacataggta taaagtaccc tgtagataca caaagtggca ctcctcacgt
     4741 tttaacaagc gactttatgc ttacagtcaa ccgcgatggg aagcagatgc agattgcccg
     4801 gactgttaag ccgtcaacag aactggagaa aaagcaagtt aatgagaaat tcgagataga
     4861 aagacaatat tacttagcaa agggtattga ctggggaatt atcacagaaa aagaaattcc
     4921 caggctttta gctgaaaatg ttgagtgggt tcattctgct tataggctag aagcaaattc
     4981 tgaaatggac actgcacaac tgagtgaatt gtcaacaatt ttaaaatcta agcttcagga
     5041 agaagataca actataaata gagtaactac tgctttagac aaagaaatga atataaattc
     5101 cggtacatca ctttatgtat ttaaacattt aattgctagg aaagaaatcc ttgtggatct
     5161 agtagcaata aaattatctg cttgtccgtc aactaaagtt attgaaaaaa taatatttta
     5221 gacccacatg ataggtaaat aatatggtta aagatttatt tgtcaatgat ttaatagagt
     5281 ggattgtata ccaaaacact catatagtcg aaagaattat ttggatagat gaaggttaca
     5341 ttattgcatt tgttatagat attaatgcta agaaaggtct acctgaacca aggaaaatat
     5401 cagatatttt agaagctata actgaaggta ttgcttttaa gcagcaacaa gatccttggg
     5461 caagaattat tcgggatgaa aatttgacag ataaggaaaa ggaatacaga gataaagctt
     5521 gggagataat ttcatcatta attatagaag aaccatcaat atattataga aattttagag
     5581 gatcgatggt taaagaagcg gttgaaaagt acaacgaaga aagggataaa gataaattag
     5641 tagaaaaaac cgtctatggt tatttgagaa gattttggca gagaggaaaa actataaatg
     5701 ctctcatacc tgattttatc aattcatcag gtaagggcaa gattcgagga caatcgggca
     5761 agaagagagg aagaccaaga aaatatgcac atgacccaga tattggtgaa ggtgtaaatg
     5821 ttacagaaga agatagaaag atttttagag tatctataag caaattttac aataatccaa
     5881 aagaaaacta cttaacaaca gcctatgaat taatggtgaa agaatattac aaggaaggaa
     5941 ttagatatga cgagaatggt gttcaaaaat ctatattaac cccaactgat aaaataccta
     6001 caattactca attcaaatac tggtatcaac ttgaacagga tgatgttacc aaaactttaa
     6061 catctcgaaa aggagccaag cgagtagctt tagagaatag agcaattttg ggaaactcta
     6121 agatggaaac tattggccct ggttctagat atcaaattga tgccacagtt gctgatgtat
     6181 atttagtttc aaaatataac cgtgactgga taattggtag accagttatc tatgtaatta
     6241 ttgatgtttt tagtagaatg attacaggtg tttatgtcgg tttagaaggc ccatcttgga
     6301 taggagcaat gatggcacta gccaatgtag ctacagataa aaccctgttt tgccaggaat
     6361 ataacattaa tattactgaa aatgaatggc cttgcaatca aatgccagat gcaattttag
     6421 gagatagggg agaattagca ggtatgacag tagaaacatt aatccctaat ctttatgttc
     6481 gtattgaaaa tgcagcttcc tatagagcag attggaaagg gttagtagaa agacactttc
     6541 gtataattca tgaatatgta aaaccttttt tacctggtta tgtggatacg gattttaggc
     6601 aaagaggtgc aagggattat aggcttgatg gcaaacttga tatagatcaa tttactgaga
     6661 ttattattcg tttaattgtt caccataaca ataaacctct tactgaatat gaaagagatg
     6721 aagcaatgat tgctgatgat acccctccta tacctagaga gttgtggaag tggggtatcg
     6781 ccaatcgttc tggcagactc agaacttttt ctgaagatat tgtcaaattg aacttgatgc
     6841 ctactgagaa agcaactatt actgctaaag gcatcaaaat gagaggtaag gagatgtact
     6901 atacctgtga tagtgctaaa aatgagcaat ggtttgaaaa agctagaagt aatctccttt
     6961 ctaagtcaga aaaatcattg attgtatctt atgacatcag aaagccaaac ttcatttatc
     7021 ttccatctag tgatggtaga gattttgaaa aatgttttct tattgactta gaagggcggt
     7081 atgctaataa aaactttcat gatattgaat atctgcttgc ttatgaagaa ttgcaaagac
     7141 agaaaagcca aggtaaacag ctacaagaaa aggtagattt aatagctgat attgaaagca
     7201 tagtagatag agcagaaaaa atgactgatg cttctcaata tcataattta agtaagagca
     7261 agaaagtttc tggaataaga gataataggg cttttgaaaa ggctaaacga cgagagaatg
     7321 aaggatttga attagcaatt acagataatc agagcagttt tcaggttgca gagagtacag
     7381 cagaaacctc cgaacagcca gaatcactac agccaaacca tctgaatctt cttaagaaaa
     7441 agcgacagga gcgaaaacgt ggacagaatt gagagtgaac tggttaaaat tcctaatgga
     7501 gaactagcag ttattgccag atatatagat caaaaactgc cagaatacaa cactaatcca
     7561 ttaattcagg cattacctcc tatcctatca gcagcagaat ttatagataa agtagccaga
     7621 gaaccaaatt ttgatgaaca agagagagag ctagaaccac attatagatt tcattgtatt
     7681 gagaggttat ctagatattt tgaccctcaa aataaaactc ttgaattaca aaaagttatt
     7741 tgtacgctta tcatgacagg ctatctgtca aggaaccttt taaaacctga atatgcaaat
     7801 cgctctaggc aaatttataa tgcaataggt aatggaggag gtaaaaattt agaaaactat
     7861 gttaatcttc ctacatctgc gtccggctta actctaattg gcccatcagg tattggaaag
     7921 actacaaatt tcaaaaatat tcttgagctt tatccccaaa taatttttca tcctgattat
     7981 agtgtttacc aaatagtttg gctgcgggta gattgcccta aaactttaaa ggggttatgt
     8041 atagatactt ttgcaggtat cgataaattg ttaggaacta atttattaaa gaaatttaac
     8101 ccagcacgca catctgaaga ttatctattg gctcaacttg cacaaacagc acacactcat
     8161 catctaggag ttttggtgat tgatgaaata caaaatttag caactgcaaa aagaaataga
     8221 gacgaaatat taaatttttt agtaaagctc gataatacta ttggaattcc agttattcgt
     8281 gtaggtacga atgaagccat tagttttctt caaggtaatc ttagaaatgc tagaagaaat
     8341 actggagaag gaagtagaat gtgggataga atggtaaata atgatgaatg gtgttacttt
     8401 ttagaaggaa tatgggaata ccaatggaca aaaacacctg ttcatttctc ggatgaaata
     8461 aaagaagttt tttatgatga aagccaggga attattgata ttgctataaa actttacaaa
     8521 atggttcaat ggagagcgat ttcaataggt gaccaagaaa ttattacggt tgatctgatt
     8581 cgtcaagtag ctaaagaagg attgtattta gtaaagccaa tgctagatgc aattaggtta
     8641 ggggacagag agtggatgat taaatataag gatattgctc ctctcgatat tacggaatat
     8701 cacaatagat gtgtatctga gattgatgct aaagatttga cagagattaa gagattagct
     8761 aaaaagcagc agaatcagca gaaagtatct ccgaagctca attatgtaat tagtgagctg
     8821 ttgaaattgg aagtagaatc tggtaaagca aaggaatgtg cagaacaaat tgtttatagt
     8881 catgaagatc cagacattcc aattccatct ttagttaata aggcatatgc tcttgctttg
     8941 caaggatgtc aagtaaatga gagtcttgct aaaacaagta ccaggaaagc taaattaaag
     9001 acaaagtacc aagagaatga tattcggcga attgtcgaga atgccaagaa agacaaaaaa
     9061 tctgcatacg aggatttgaa attagcaggt ataatcaaaa atccagttga agattttatc
     9121 aatatttagc ttctaaatgc ttagtttttt cccaactctg tatcccgatg aacttttata
     9181 tagcacttta gctagatacc atattcggag tggaaataaa agtttcaagc aaactgactt
     9241 agagctattc ggttacagtt cccaacaaat atgcaaaatt accttaacta ataatttaaa
     9301 ttatttagtg aaaaacttac ctttacgctc ccagcaaaaa gttgagaatt tactacaaaa
     9361 tcatacttta tattcctttt atgcaacttt attaatgccg caggaggctt ggctgctgaa
     9421 aaactcaatg agtaataaat tcagtgggtc tatactagat tttgctaaag tggctaataa
     9481 ttctagtgat gattcaaaaa aatttttaaa attttgccct gaatgtttag agaaagatac
     9541 ccaaaagtat ggtgaacctt actggcatag aatacatcaa gtccctggca ttttagtttg
     9601 tccaattcat cgtgttgttt tacacaatag ctgtatagaa gttgaatcga aaggcataca
     9661 ttatcatgct gccagcatag ataattgttt attagcaaat aatgtgaaaa aattgacaaa
     9721 taaaactgta gaaacacttt caatactggc acatgatatt aattggctaa ttcatagcag
     9781 ttttgccttt caaggtttgg cttggcttcg cgctcagtac cagcgttact tattggataa
     9841 aggactgcta aaaacctttg ctggtggcaa gtttaagttt aatgattgtg gttttgccca
     9901 gcttatattt gagttttatg gtcaagaatc cttagaagcc ctcaatccaa agctgaccca
     9961 aaatcaaggt aaatattttt ctcactgctt gtttggatgc gacttaaacc cagcaattga
    10021 ccgcataact catatcttaa tgataaaatt tttagcaaat tctatagaag agttctttca
    10081 gtcataactt aagtcgaaca gaaagtaatc gtattgtaca ccatcgtggc cggatcttgc
    10141 ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc
    10201 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
    10261 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
    10321 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
    10381 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
    10441 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
    10501 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
    10561 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
    10621 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
    10681 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
    10741 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
    10801 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
    10861 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
    10921 tgatagttga gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata
    10981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11041 gaaaaataaa caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg
    11101 ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg ggactaacat
    11161 gtgaggcaaa acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc
    11221 agagttcaca taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat
    11281 gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg
    11341 tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct
    11401 ttctccctta cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg
    11461 tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct
    11521 gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac
    11581 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
    11641 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
    11701 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
    11761 cacctcccca ggtggttttt tcgaaggtta agtcagttgg ggaactgctt aaccttcgat
    11821 caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa
    11881 aggatctcaa gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt
    11941 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg
    12001 ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
    12061 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
    12121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    12181 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
    12241 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
    12301 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
    12361 gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
    12421 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
    12481 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
    12541 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
    12601 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
    12661 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
    12721 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
    12781 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
    12841 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
    12901 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
    12961 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
    13021 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
    13081 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
    13141 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
    13201 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
    13261 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat
    13321 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
    13381 tgtttaactt taataaggag atataccatg gtgctttaac attagatgtc gttaggcgtt
    13441 gagcagagaa gtcatttaat aaggccactg ttaaacgtag atgtgcttta acattagatg
    13501 tcgttaggcg ttgagcagat ccgaatt
//
