LOCUS       sequence-33070-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP318
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..56)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        68..288
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 gtcgactcta gaggatcccg ggataacttc gtatagcata cattatacga agttatgcac
       61 cacgcttttc aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
      121 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
      181 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
      241 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct
      301 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
      361 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
      421 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
      481 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
      541 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
      601 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
      661 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
      721 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
      781 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
      841 caaagagaca t
//
