LOCUS       sequence-33071-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP320
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        165..352
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 cccgggataa cttcgtatag catacattat acgaagttat cgttttaaga gcttggtgag
       61 cgctaggagt cactgccagg tatcgtttga acacggcatt agtcagggaa gtcataacac
      121 agtcctttcc cgcaattttc tttttctatt actcttggcc tcctctagta cactctatat
      181 ttttttatgc ctcggtaatg attttcattt ttttttttcc acctagcgga tgactctttt
      241 tttttcttag cgattggcat tatcacataa tgaattatac attatataaa gtaatgtgat
      301 ttcttcgaag aatatactaa aaaatgagca ggcaagataa acgaaggcaa agatgacaga
      361 gcagaaagcc ctagtaaagc gtattacaaa tgaaaccaag attcagattg cgatctcttt
      421 aaagggtggt cccctagcga tagagcactc gatcttccca gaaaaagagg cagaagcagt
      481 agcagaacag gccacacaat cgcaagtgat taacgtccac acaggtatag ggtttctgga
      541 ccatatgata catgctctgg ccaagcattc cggctggtcg ctaatcgttg agtgcattgg
      601 tgacttacac atagacgacc atcacaccac tgaagactgc gggattgctc tcggtcaagc
      661 ttttaaagag gccctagggg ccgtgcgtgg agtaaaaagg tttggatcag gatttgcgcc
      721 tttggatgag gcactttcca gagcggtggt agatctttcg aacaggccgt acgcag
//
