LOCUS       sequence-33072-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP322
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccgggataa cttcgtatag catacattat acgaagttat taaccattat ttttttcctc
       61 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
      121 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
      181 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
      241 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
      301 ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca
      361 atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt
      421 tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc
      481 tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc
      541 tgctatcgaa gctacaggtg tcccacttcc agatgaggcg ctggaagcct ccaagaaggc
      601 tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtacaggta gtgttagacc
      661 tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg
      721 taactttgca tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg
      781 tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta agagaaag
//
