LOCUS       sequence-330720-       12839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins (except TnsD). Two BsaI 
            sites for spacer cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 12839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS23: pHelper(PmcCAST)_entry_Delta-TnsD
FEATURES             Location/Qualifiers
     source          1..12839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    43..67
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(68..86)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    329..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(354..372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        418..495
                     /gene="lacI"
                     /label=lacI promoter
     CDS             496..1578
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(515..534)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        2639..2730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2731..3522
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(3643..3690)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4758..4782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4783..4801)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8747..8771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8772..8790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(8936..8983)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgtatatct ccttcttata cttaactaat atactaagat ggggaattgt tatccgctca
       61 caattcccct atagtgagtc gtattaattt cgattatgcg gccgtgtaca atacgattac
      121 tttctgttcg acttaagcat tatgcggccg caagcttgtc gacctgcagg cgcgccgagc
      181 tcgaattcgg atcttgagca acgcctaacg gcataaagcc acgactcact gagaccagag
      241 gtctctttga gcaacgccta acggcataaa gccacgactc accatggtat atctccttat
      301 taaagttaaa caaaattatt tctacagggg aattgttatc cgctcacaat tcccctatag
      361 tgagtcgtat taatttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac
      421 accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
      481 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      541 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      601 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      661 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      721 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
      781 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
      841 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
      901 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
      961 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
     1021 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
     1081 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
     1141 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
     1201 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
     1261 gccattaccg agtccgggct gcgcgttggt gcggacatct cggtagtggg atacgacgat
     1321 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
     1381 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     1441 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     1501 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1561 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc
     1621 gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     1681 gggcatgact aacatgagaa ttacaactta tatcgtatgg ggctgacttc aggtgctaca
     1741 tttgaagaga taaattgcac tgaaatctag agcggttcag tagaaaagat caaaggatct
     1801 tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg
     1861 ggaggtggtt tgatcgaagg ttaagcagtt ccccaactga cttaaccttc gaaaaaacca
     1921 cctggggagg tggtttgatc gaaggttaag tcagttgggg aactgcttaa ccgtggtaac
     1981 tggctttcgc agagcacagc aaccaaatct gtccttccag tgtagccgga ctttggcgca
     2041 cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc tgcgaactcc cagttaccaa
     2101 tggctgctgc cagtggcgtt ttaccgtgct tttccgggtt ggactcaagt gaacagttac
     2161 cggataaggc gcagcagtcg ggctgaacgg ggagttcttg cttacagccc agcttggagc
     2221 gaacgaccta caccgagccg agataccagt gtgtgagcta tgagaaagcg ccacacttcc
     2281 cgtaagggag aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc
     2341 aagagggagc gacccgccgg aaacggtggg gatctttaag tcctgtcggg tttcgcccgt
     2401 actgtcagat tcatggttga gcctcacggc tcccacagat gcaccggaaa agcgtctgtt
     2461 tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg cggccctgct
     2521 gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgtatgt
     2581 gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga gctagctatt
     2641 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2701 atgcttcaat aatattgaaa aaggaagagt atgagggaag cggtgatcgc cgaagtatcg
     2761 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     2821 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     2881 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     2941 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     3001 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     3061 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     3121 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     3181 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     3241 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     3301 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     3361 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     3421 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     3481 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca
     3541 agccgagggg ccgcaagatc cggccacgat gacccggtcg tcggttcagg gcactaccgg
     3601 aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc agcaaaaaac ccctcaagac
     3661 ccgtttagag gccccaaggg gttatgctag ttattgctca gcggtggcag cagcctaggt
     3721 taattaacta taactttaat tccagactaa aattcccgaa caatatcttg cccaattcct
     3781 taaaatctga cccaaacaac tggaatacac taatcttcgc cttctcgcac acaggaataa
     3841 tcaacttcac caaatgataa ggttgacttt gatttgttgt tgaggctatc ctcaacgatt
     3901 ccatgattcc tcctaaatct cccaataact tcacatcctt tccaattgct tgttcaaaca
     3961 ctaacaaaat caattgtaat tttcgggtta actcgatcgg gatgggttta taacctaatt
     4021 ggggagtaag tgcaattaat tcgctaattc cttgaatatt aactcgatat ttctctattt
     4081 cttcttcgcc tttaatttcc tgtcctaacc acccgtaaca acgagaacaa aatcccgcag
     4141 gtgaaagcct agccatcaca ggtaaacggg caccacaatg agaacattct tctattaatc
     4201 gctgtttgtg aatcaaacaa aattctacgt ccttaaatga ccatgataat ggttcataga
     4261 tagttttgtt cttctgcatc cactcttcac aacaacaagg acaccaagcg cgatagttcc
     4321 gaaataatcc cttgtcataa atcattcctt tccagcttaa aagggtcaaa aagcgtaaat
     4381 cctgacgcat tgttaactct tctaagacag tgactaactt ttctgtcatt tctcgcattc
     4441 cgttaatagc cggtttagcg tcgctatttc ccaataggtg actcagattt tttgacaata
     4501 gctcagattt atcctcatct ttgagaataa gtggcgcaat ttctcccata acgagcttct
     4561 cagaggttaa acaatgcgcc tgcgctaaac gatggagata gctactaaga ctttctgcat
     4621 agggagttcc cactgccaca ggttcaagag aaaataaccg actccgttgc gggatttcta
     4681 gaggatttag gctccaatgt tctgagtaaa tagtcatgta tatctccttc ttatacttaa
     4741 ctaatatact aagatgggga attgttatcc gctcacaatt cccctatagt gagtcgtatt
     4801 aatttcgatt atgcggccgt gtacaatacg attactttct gttcgactta agctagctaa
     4861 catcttgctg cactccgatg ggatctcttt taggtttgcg tttgccaacc tttcctggcg
     4921 gacgagtagt ttttggggtt tcttcgggta gcacaattgg ttttgcgcca agtcctaatg
     4981 cagagcgtaa gttttgcaca tctgcttctg tttcagataa ttgtcgttca ccctcttgaa
     5041 tttccttgaa cattttctga cactgagcaa ccgacaaagc acgtttttgt aaatctttga
     5101 gagtgattgt tgttgcttct cgatccaaag catcactaag aacacgcttc agccaatctt
     5161 tcagtgtgcc aatacatcct aaagtacgtt cgtaaaaata ttcccaatga tcgactaaat
     5221 ttggagtttc tgctagtggc agatgctgtt gaaaagttaa caacacactc ttaaaagcct
     5281 gtacatcttc tgggctatca gcacaataac ggcggaaatg aatatcaaca ctacgacgtg
     5341 agagttgacc gctcaaatta cggaatgtaa gtagttcgta tgttcctaat agacagtgca
     5401 agatgcctgt catatttgct aaagatttga gacagtctaa ttgatcttgt agtttgtatc
     5461 cgcttgccac cttaccaaaa tgttgtgctt catctacaaa aaatacgtct ggatgacgat
     5521 ggatgagagc attttctaaa gctctgcgta gagcaggtgc aactactttc gactcaacat
     5581 taattttgcc aaaattatcg cgagagatac cacgcacacc gtaatcaaac ttgtgatcga
     5641 ttaatggttc ttccaaagta attaaggcgc gtgtgtagta ttctttccag ttgaagtatc
     5701 tcgattctgg cgctatagct tcaataccaa caacaggaac acgagcgcga tcgctttcca
     5761 atttaggtaa tgctagttct gttaatttct gctcaacgcg gagtcgcaaa gttgttttac
     5821 caacaccact agcaccgtaa acaaagataa atgaagctcc tgctggttcg gcaatcgttc
     5881 gcatgagaat ttcatagact tctttaagtc gaggatgcgc tacggtgtaa ttttcaaagt
     5941 aagcaagcct ttctgtagca ggccgtgtga ggagttcaag cggaaatcca gtagattttg
     6001 tcatagttaa aactcctcat acactacaag tgtttctggg acgatcgctt catcttcatc
     6061 cgcttgaaaa tcggtattgg caaaagtaac ttgaataagt tttggttctt cgttacggct
     6121 tgttgtcgct tttccaccct cgattaccct aaagacttcc ttaacttctg catcatagga
     6181 acgctgttct aatagagctt cttgagcttc taccgatgct aaaaattctg ctaaattctt
     6241 agccgaaact ttagattgtt gtgtatgatt tgaatagtgt ttgcgtaact cgatactagc
     6301 taacttcagt tctttttctg aacgaccttg gagttctgca taatattgag agatacactg
     6361 tacccactgt cctcgaacaa aggcgtaagc gatgccaata ttaaacgggt cgtatcttat
     6421 ctgcacagag gtattttcaa tttgaggatc gcggaaacta ttagaccagt agtatatgta
     6481 gttaattttt atgccgcgtc caacttgaac ttttgctttt ccctctgagg ttgtaggtag
     6541 ggtaagaatc tgaaagtttt catcgtaagg aatcatgcgg tgaacccgac tgcctccaat
     6601 cgccataccc gcagcaaacg catccctcgg actctggaaa agtgccggat gttcgtctgt
     6661 atcataaact tcgtaagccc aagcacaaag atattcataa agtaaaccaa gtgtccatac
     6721 agctagattt ttagggttaa ctgacttagt gacttggcgg acattacgag tgatttgtgt
     6781 attaccaagt aagttatgga caaattgagt attggaagtt ccaaataggc gttcgcaaac
     6841 cgaaccaaaa cgagctttgg ctgggggtct ttgctttttt gtacattcaa aagtagctag
     6901 tagtgtttca aagtaaacgc tgtgaaactc tgcaccgtta tctaccacta caatttgggg
     6961 aagcctgcca tgacgcttga cacaaattct tagcaccatt aagcaagaac gatatgaggg
     7021 tgagtcaaac gttaggtaaa ctgccagcaa tcgccgtgaa taagcatcta cgagaaatgt
     7081 tgcccaaggt ctgccaagat tgcgacctgt atcagagcaa actagctcta catctaactc
     7141 agtatgatct atatgcccaa tttcaaaagg gcgatcgccg tgtcttgggg tagttatagc
     7201 aagctcccaa tagaaggatt catgctgata agcggcacga cgaccttggc gttttttagt
     7261 ctgttcgtaa ccagtccggc gttgtacttc tttggtaaat gctttatagc tcggagcaat
     7321 tacaccagct tcttcgcaag ctcgaactaa agccgcgtga acctcccaca ttcttttttg
     7381 tttaagagtt tcatagtctt ctaaaataaa cttctccata attgctaagg tgtcttcggg
     7441 cagcttgcgc tgtcgattac ctttgacact gcgatgggac aaaagcccaa tgtagccgca
     7501 accgtatttt tgaattgcaa ttaaatattt agctttccag tgacgaatgg tacgggctgg
     7561 tattgtttcg ttttctggag gatgtccgtt taagtaaggt tcgatagttt tgtagcgttc
     7621 aagggcttct gcttggtctt caggactagc ttgatgaaat cgctgccaag attctgtgct
     7681 aatagctgtt ttttctggtg tttggagact tgtgattttt ccttggcgaa ccagattctc
     7741 aaatatagct tttttgagtt caattaactg attattttct gcgccaagta taacctctgt
     7801 ttctccaaca tggatgatat ttaaaccttt gccgtcccaa ctaacagttg ctccagtaac
     7861 aatattgact acaggagaga tagccggaag acttacactt ggttgctcaa ccattaatct
     7921 ataagcacta gcagtttgct cgtcacggaa gattagacat ctttctggtt cagctagtgg
     7981 tgtagcattt aaatcaatat agatttgctc ttgaataatt aaggtgtaga tatcgtcaga
     8041 tttagctcct gttgaccgat tgagtaattc tgctaaagtg attcctggtt gagatgacac
     8101 aattgcaagt aatgattgag cgatcgcttc ttccactact agagattctg agcgataata
     8161 atcttctaaa aattgcaaat tacgttgcag aacccaattg atttcgcgat ctgaatgaat
     8221 gcggtgataa agcccaaact ggttagcata aacttctcct ggcaaggaat gccattgatt
     8281 attttcttca gagtaaaagt aacgattggg gtttttctct gctagttttt tcaaatcctg
     8341 ctcagtttta cactcttccc aaccagccga attggttcgg atgacaaata aatctggggt
     8401 gtgtaaaacg cctaatctac gtccgttact tgcttgatag tcaagtttaa tttgcggtgg
     8461 ttgatcgtag aattctagaa catctttatc atgttctaac tgatagataa ttggcagttc
     8521 aacccgatga gactcaaatt gaatcgtgac tcccatcttg cgactagggt agcgtcccga
     8581 aacattcttg ctaccgccac caacgcgacg ggatggctca gatgagcgaa ctttctcaat
     8641 aattttttgc cctgcctcgg atatttgaag gcgagcaacc cattgttcaa attgctcttt
     8701 catggtatat ctccttatta aagttaaaca aaattatttc tacaggggaa ttgttatccg
     8761 ctcacaattc ccctatagtg agtcgtatta atttcctaat gcaggagtcg cataagggag
     8821 agcgtcgaga tcccgagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
     8881 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggt ttcagcaaaa
     8941 aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc tcagcggtgg
     9001 cagcagccta ggtcaaatcg gagattgaac cattgtccag gctaaatcag gaggtgataa
     9061 tctatccatt tcctcaatcc gatacctcaa aaatcgggta tttcttgacc ccacatggtc
     9121 aacccaatag ggtaaagtta acctgcctcg attatctcta atcgcccact gtctcactcc
     9181 ctctagataa tcttcccttg caagtttgat cgttttaatc aactggtcac tttctcccaa
     9241 aaataaacaa ccaaaccgtc ttactagctc aggatggtca aacgctaact gtatcctctc
     9301 ccttaaactt ggttgtatct tttcctcgtc agaacgaacc caaatcaaac acttcagatt
     9361 agacaaaatt tcttgataac agggaataac attctctgga tggctaaaat cagcattctt
     9421 aaaccgtctc atttgacgca aaattcgcga ctttttgggg ctagataaca ttgcaatggc
     9481 caattctacc ccacagtggc gatagacatt cgtttctcct accaaagaca acaacatccc
     9541 atacactgtc gctggaggag gaacaggata ggtttttccc atttcccttg catgggactc
     9601 ccgaaaagtc gtaaatggaa catccaaata gagaattaat ggtgcaatca ttttgattct
     9661 tccaagccca aatctctttt aatgactgtt ttcaaagctt caatcatttc atttcgatta
     9721 cgataaatat gcgccttgtt caaaggggaa ctttcaaaat tatccaattg ctgtaaatcc
     9781 ttaacaattg ttcccccaat ccacaattta ctagggtcaa tctgtccgca ttccacttca
     9841 tctataaact cttgtgaaag ttttgcttcc ttactcttag gatcgcaata ttcaaagcaa
     9901 taggaaatat atgaagcaaa atgatttgtc cattgaaata ccaaactatc gggacagaaa
     9961 ggataattaa aaatattact actccctcct accttgggca gattcataat cccatctatc
    10021 ataggtaaaa tccgcgaaaa atctttgaga tgagtggcat caattccaaa ataatattga
    10081 tatcgagttg catgatattc tgtaaaatgg agagatgtac tatccttctc tctgccactt
    10141 tttgccccta acttaactgc accatcataa ggcgttagcg aaacagccat attcatccct
    10201 aaagcaccca ttcgctgatt aggagtgctg gtctttgttt gtttcttttt tctctttgtt
    10261 gttgaagtgt cctcctctgt ttcagcagac tctaataaag caaacccaaa aatatcatca
    10321 tcataaaatt tttctggatc gaaatcttca cttgttaacc gattaatatg ttcttcctca
    10381 tcccagactc gattaactaa ataaccttgc ttttgaagat aaaaacgtaa cgcccaacga
    10441 attgcacttg aacctacaaa agaatgaatt ttaccattat tccaatagcc tttttgcaga
    10501 atagtcttat ttcctcgtct aatgtcatga tttaaggaag ctaaaccata acgagtgagg
    10561 actgttccgt aaagataata atttgggata ttactgtcat tcttcttctg agtcatcatt
    10621 cacttcttct agtttttgat tgtttgacaa tgaactatca tccctattag tgagtttatc
    10681 tttgggttta taactggcga tcgctaacaa tgaccaaatt cttatttctt gccaaggtga
    10741 ttttttaatt agtagtgcaa tctcttcctg atgctcatta aaatacttat ttaatccccc
    10801 tcttactaaa aagtcagaca aatattcctt aaacgacaat tcatcataac aatagtttag
    10861 ctctgccctt aatcgttcaa ctttcttctt aattggagga tctttacctt cctctgtttt
    10921 ggcataaatt ttagcaaagt tgcccttcat agcttgctga aataccttaa taaaaataag
    10981 atactgcttg tcttcttcac tattctctgc cattatgatg aatccttctc gattggaaaa
    11041 taactggtta aataaatatt cttttgaatc ctctatcacc aatttttccc aaaaattaga
    11101 ccaccaatgg attcctttca ctaaattatc agcaatcagg gagcgaatcg gattgacctt
    11161 aataaaaatt tgcttatatt taattatctg ataattagtt ttaaagtatt tttgcacctg
    11221 ttgataagtg attaaaatat tcttatcaat tttaatttct tctatactca tcaatgtccg
    11281 ttgacgagaa gctttatttg ttttttcata taaccaaact tgacaagctt gatagtaggc
    11341 cacttcaggc tgaatatcgt ctaagctgta ataaagtaat cctgcttccc caaggctact
    11401 aacatgaaac tgtttagttt ctaattgctg taatcgccat ctcctttgag aagcgtcttc
    11461 aaaatccttt atttctggaa tcaccaccaa gtagcgatgg ggtttccttt cttttaagtc
    11521 ttctgatggt atatgaagta agcaataatg acaaactact ggtacaaata ataatgctaa
    11581 agcgtattca ggtttctctt ctagttttgt agtattctgc gtccgagcat ggcgaacaat
    11641 tccccctaaa tacaaccaac tggttatttg aatgtaatca tgtctgagtt gttgggtatc
    11701 tgcctcgcat aacttctctg caaaggtttg atgtgcgtac caagttaggg atttatattg
    11761 atactcgact tgtttttctt caaaccttag ttcagtgtta attatctctc ctgcttgata
    11821 aaacttatta agacgtagaa aaacaccgca tattccctca tgaatatgga ttttctgcct
    11881 taaatctatt cgatcattgt ctaatcctac taaatgaatt aaacctgtat catctaattg
    11941 aaaagactca ttaattaacc aagataaggc gatgaaatca cttccttccc agaataattc
    12001 aatagtatca gcagtcaaaa accatgatat atgtcctccc cgttgacgag ataagggata
    12061 ttgcttttcc aatcgcttta aagtcatgta aagtccggtc attccgacgc ggtgcattat
    12121 tgtcgtatcc gcagcatgga gagataggga aattttgggt tgggtagaga ctatcatcag
    12181 gtttcctctt cttccttgct gcgagtcgct gggacaaata ttccacacat catctttcgt
    12241 ttcccgccaa ttccctgttc ttgcaaggtt aaggaatctt caggattgag ttcactgatt
    12301 tttacgccaa atcctctaac tttaaactgt ttcccttctt tattgatggt taattgtctt
    12361 cgttgaggtg ttccatcttg tcgggtaaga agttcaattt ttccttgtat tcctaaatta
    12421 tccatttgcc gttgcacagc ttcaataaag ttagtagagt cttggaaccc tttaattatc
    12481 actaatctgg aatagacact ttctgaagaa attaacggtt tgtagtcggg gatatctagt
    12541 tgataaaaat tttgacctat atgaaaagct tgacccgcta aataaggata aattaaagga
    12601 atttgctgat gataaatccg aatttttaga cgagattgag cagttagctc aagcaaattg
    12661 ttcctggtcg gtattccagc aatcggatga atcccaatag actttagttc atgtagtggg
    12721 ggacaaatac gggataaggc agcgtaggta agataaccgt tgtcaagagg aatcggtgcg
    12781 cctcttagct taaatgttaa gtctacatat tcctcacttt gaattaatat agctgtcat
//
